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Environmental Science and Pollution Research

, Volume 25, Issue 9, pp 8836–8842 | Cite as

Supplementing dietary rosemary (Rosmarinus officinalis L.) powder and vitamin E in broiler chickens: evaluation of humoral immune response, lymphoid organs, and blood proteins

  • Hossein Rostami
  • Alireza Seidavi
  • Mohammad Dadashbeiki
  • Yadollah Asadpour
  • João Simões
  • Assar Ali Shah
  • Vito Laudadio
  • Caterina Losacco
  • Antonella Perillo
  • Vincenzo TufarelliEmail author
Research Article

Abstract

The aim of the present study was to evaluate the effect rosemary (Rosmarinus officinalis L.) powder (RP) and vitamin E (VE) at different levels on humoral immunity of broilers during a 42-day production cycle. A total of 270 1-day-old male chicks were assigned to nine groups with three replicates of ten birds each, and diets were supplemented with 0, 0.5, or 1.0% RP and 0, 100, or 200 mg/kg VE, respectively. Commercial-inactivated vaccines against avian influenza (AI) and Newcastle disease (ND) viruses, and living infectious bronchitis virus (IBV) vaccine were administered by spray method. Sheep red blood cells (SRBC) were administered subcutaneously. Blood samples were collected from birds 1 week after each vaccination to determine antibody titers. At the 42nd day, blood samples were also assessed for globulin level, and lymphoid tissues (thymus, spleen, and bursa) were weighed. Neither antibody titers against viruses nor lymphoid tissues weight were affected by RP and/or VE (P > 0.05) treatments. However, broilers supplemented with 0 mg/kg of VE had lower antibody titers against SRBC than those fed 100 mg/kg of VE (P < 0.05) at the 24th day. A significant RP × VE interaction effect (P < 0.05) on plasma globulin level was observed. The findings of our study suggest that dietary RP and VE additives can interact and modulate the humoral immunity of broilers, but not sufficiently to improve antibody titers against specific virus during a 42-day production cycle.

Keywords

Chicken Immunity Rosemary Vitamin E Diet 

Notes

Acknowledgements

Financial support by Rasht Branch, Islamic Azad University, grant number 4.5830, is gratefully acknowledged.

Compliance with Ethical Standards

The study was approved by the ethics committees of the authors’ institutions.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hossein Rostami
    • 1
  • Alireza Seidavi
    • 1
  • Mohammad Dadashbeiki
    • 2
  • Yadollah Asadpour
    • 3
  • João Simões
    • 4
  • Assar Ali Shah
    • 5
  • Vito Laudadio
    • 6
  • Caterina Losacco
    • 7
  • Antonella Perillo
    • 7
  • Vincenzo Tufarelli
    • 6
    Email author
  1. 1.Department of Animal Science, Rasht BranchIslamic Azad UniversityRashtIran
  2. 2.Department of Veterinary Science, Rasht BranchIslamic Azad UniversityRashtIran
  3. 3.Agricultural and Natural Resources Research Center of GuilanRashtIran
  4. 4.Department of Veterinary ScienceUniversity of Trás-os-Montes e Alto DouroVila RealPortugal
  5. 5.College of Animal Science and TechnologyNanjing Agricultural UniversityWeigangChina
  6. 6.Section of Veterinary Science and Animal Production, Department of DETOUniversity of Bari Aldo MoroValenzanoItaly
  7. 7.Department of Veterinary MedicineUniversity of Bari Aldo MoroValenzanoItaly

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