Environmental Science and Pollution Research

, Volume 24, Issue 29, pp 23226–23235 | Cite as

Microbial community composition and electricity generation in cattle manure slurry treatment using microbial fuel cells: effects of inoculum addition

  • Binghan Xie
  • Weijia Gong
  • An Ding
  • Huarong Yu
  • Fangshu Qu
  • Xiaobin Tang
  • Zhongsen Yan
  • Guibai Li
  • Heng Liang
Research Article


Microbial fuel cell (MFC) is a sustainable technology to treat cattle manure slurry (CMS) for converting chemical energy to bioelectricity. In this work, two types of allochthonous inoculum including activated sludge (AS) and domestic sewage (DS) were added into the MFC systems to enhance anode biofilm formation and electricity generation. Results indicated that MFCs (AS + CMS) obtained the maximum electricity output with voltage approaching 577 ± 7 mV (~ 196 h), followed by MFCs (DS + CMS) (520 ± 21 mV, ~ 236 h) and then MFCs with autochthonous inoculum (429 ± 62 mV, ~ 263.5 h). Though the raw cattle manure slurry (RCMS) could facilitate electricity production in MFCs, the addition of allochthonous inoculum (AS/DS) significantly reduced the startup time and enhanced the output voltage. Moreover, the maximum power (1.259 ± 0.015 W/m2) and the highest COD removal (84.72 ± 0.48%) were obtained in MFCs (AS + CMS). With regard to microbial community, Illumina HiSeq of the 16S rRNA gene was employed in this work and the exoelectrogens (Geobacter and Shewanella) were identified as the dominant members on all anode biofilms in MFCs. For anode microbial diversity, the MFCs (AS + CMS) outperformed MFCs (DS + CMS) and MFCs (RCMS), allowing the occurrence of the fermentative (e.g., Bacteroides) and nitrogen fixation bacteria (e.g., Azoarcus and Sterolibacterium) which enabled the efficient degradation of the slurry. This study provided a feasible strategy to analyze the anode biofilm formation by adding allochthonous inoculum and some implications for quick startup of MFC reactors for CMS treatment.


Microbial fuel cell (MFC) Cattle manure slurry (CMS) Microbial community structure Inoculum Startup time 



The authors would like to specially thank the supports by the Scientific and Research Foundation for Returned Scholars of Heilongjiang Province (LC2016015), the Young Talents Project of Northeast Agricultural University (14QC35), the Specialized Fund Project for Harbin Innovative Talent in Science and Technology Research (2013RFQXJ094), and the HIT Environment and Ecology Innovation Special Funds (HSCJ201603)


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Binghan Xie
    • 1
  • Weijia Gong
    • 2
  • An Ding
    • 1
  • Huarong Yu
    • 1
  • Fangshu Qu
    • 1
  • Xiaobin Tang
    • 1
  • Zhongsen Yan
    • 1
  • Guibai Li
    • 1
  • Heng Liang
    • 1
  1. 1.State Key Laboratory of Urban Water Resource and EnvironmentHarbin Institute of TechnologyHarbinChina
  2. 2.School of EngineeringNortheast Agriculture UniversityHarbinChina

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