Variation of preserving organic matter bound in interlayer of montmorillonite induced by microbial metabolic process
- 141 Downloads
This paper aimed to investigate the variation of preserving organic matter bound in the interlayer space of montmorillonite (Mt) induced by a microbe metabolic process. We selected Bacillus pumilus as the common soil native bacteria. The alteration of d 001 value, functional group, and C,N organic matter contents caused by bacteria were analyzed by XRD, FTIR, and elementary analyzer, respectively. XRD results showed that the d 001 value of montmorillonite increased with the concentration decreasing and decreased with the culture time increasing after interacting with bacteria indicating the interlayer space of montmorillonite was connected with the organic matter. The findings of long-term interaction by resetting culture conditions implied that the montmorillonite buffered the organic matter when the nutrition was enough and released again when the nutrition was lacking. The results of the elementary analyzer declared the content of organic matter was according to the d 001 value of montmorillonite and N organic matter which played a major impact. FTIR results confirmed that the Si-O stretching vibrations of Mt were affected by the functional group of organic matter. Our results showed that the montmorillonite under the influence of soil bacteria has a strong buffering capacity for preserving organic matter into the interlayer space in a short-term. It might provide critical implications for understanding the evolution process and the preservation of fertilization which was in the over-fertilization or less-fertilization conditions on farmland.
KeywordsMontmorillonite Bacillus pumilus Interlayer spacing Organic matter preservation Metabolic process
This work is supported by the National Natural Science Foundation of China (No. 41130746), the Natural Science Foundation of Southwest University of Science and Technology (No. 12ZX7121), and the Applied Basic Research Project of Science and Technology Department of Sichuan Province (No. 2016JY0213). We also are grateful for the help of the Analytical and Testing Center of Southwest University of Science and Technology.
- Hatta T, Echigo T, Nemoto S, Tamura K, Yamada H (2011) Intercalation of sugar alcohols into the interlayer of montmorillonite by wet and dry processes. Clay Sci 15:61–66Google Scholar
- Jiang W, Yang K, Vachet RW, Xing B (2010) Interaction between oxide nanoparticles and biomolecules of the bacterial cell envelope as examined by infrared spectroscopy. Langmuir 26(23):18071–18077Google Scholar
- Swann GEA, Patwardhan SV (2010) Application of Fourier Transform Infrared Spectroscopy (FTIR) for assessing biogenic silica sample purity in geochemical analyses and palaeoenvironmental research. Clim Past Discuss 6(5):1629–1653Google Scholar
- Yu BS, Dong HL, Han PY (2012) Experimental research on microbial degradation of organic matter adsorbed in smectite internal surface area in the interlayer of the structure. Acta Petrol Sin 28(3):949–960Google Scholar
- Zhang G, Kim J, Dong HL, Sommer AJ (2016) Microbial effects in promoting the smectite to illite reaction: role of organic matter intercalated in the interlayer. Am Mineral 92(8–9):1401–1410Google Scholar