Environmental Science and Pollution Research

, Volume 25, Issue 22, pp 21296–21307 | Cite as

Organophosphorus pesticide mixture removal from environmental matrices by a soil Streptomyces mixed culture

  • Gabriela Briceño
  • Karen Vergara
  • Heidi Schalchli
  • Graciela Palma
  • Gonzalo Tortella
  • María Soledad Fuentes
  • María Cristina Diez
Advances in Environmental Biotechnology and Engineering 2016


The current study aimed to evaluate the removal of a pesticide mixture composed of the insecticides chlorpyrifos (CP) and diazinon (DZ) from liquid medium, soil and a biobed biomixture by a Streptomyces mixed culture. Liquid medium contaminated with 100 mg L−1 CP plus DZ was inoculated with the Streptomyces mixed culture. Results indicated that microorganisms increased their biomass and that the inoculum was viable. The inoculum was able to remove the pesticide mixture with a removal rate of 0.036 and 0.015 h−1 and a half-life of 19 and 46 h−1 for CP and DZ, respectively. The sterilized soil and biobed biomixture inoculated with the mixed culture showed that Streptomyces was able to colonize the substrates, exhibiting an increase in population determined by quantitative polymerase chain reaction (q-PCR), enzymatic activity dehydrogenase (DHA) and acid phosphatase (APP). In both the soil and biomixture, limited CP removal was observed (6–14%), while DZ exhibited a removal rate of 0.024 and 0.060 day−1 and a half-life of 29 and 11 days, respectively. Removal of the organophosphorus pesticide (OP) mixture composed of CP and DZ from different environmental matrices by Streptomyces spp. is reported here for the first time. The decontamination strategy using a Streptomyces mixed culture could represent a promising alternative to eliminate CP and DZ residues from liquids as well as to eliminate DZ from soil and biobed biomixtures.


Chlorpyrifos Diazinon Degradation Enzymatic activity Soil Biobed biomixture 



The authors acknowledge the financial support from the National Fund for Scientific and Technological Development, FONDECYT Project N° 11130716 and FONDECYT Project N° 1161481; and the National Commission for Scientific and Technological Research (CONICYT)—Ministry of Science, Technology and Productive Innovation (MINCYT), Programme of Scientific International Cooperation (Chile-Argentina) PCCI 140056.


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Gabriela Briceño
    • 1
    • 2
  • Karen Vergara
    • 1
  • Heidi Schalchli
    • 1
    • 3
  • Graciela Palma
    • 4
  • Gonzalo Tortella
    • 1
    • 2
  • María Soledad Fuentes
    • 5
  • María Cristina Diez
    • 1
    • 3
  1. 1.Centro de Excelencia en Investigación Biotecnológica Aplicada al Medio Ambiente, Facultad de Ingeniería y CienciasUniversidad de La FronteraTemucoChile
  2. 2.Scientific and Technological Bioresource Nucleous (BIOREN-UFRO)Universidad de La FronteraTemucoChile
  3. 3.Departmento de Ingeniería QuímicaUniversidad de La FronteraTemucoChile
  4. 4.Departamento de Ciencias Químicas y Recursos NaturalesUniversidad de La FronteraTemucoChile
  5. 5.Planta Piloto de Procesos Industriales Microbiológicos (PROIMI-CONICET)TucumánArgentina

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