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Environmental Science and Pollution Research

, Volume 25, Issue 22, pp 21272–21285 | Cite as

Monitoring and modeling 4-chlorophenol biodegradation kinetics by phenol-acclimated activated sludge by using open respirometry

  • Cintia C. Lobo
  • Nora C. Bertola
  • Edgardo M. Contreras
  • Noemí E. Zaritzky
Advances in Environmental Biotechnology and Engineering 2016

Abstract

The aim of this study was to analyze the mechanisms, stoichiometry, and stability of 4-chlorophenol (4CP) biodegradation kinetics by phenol-acclimated activated sludge using open respirometry. While the removal of 4CP was higher than 98%, the removal of chemical oxygen demand (COD) ranged between 69 and 79% due to the accumulation of an intermediate metabolite. The value obtained from respirometric profiles for the stoichiometric ratio of O2 to 4CP (YO2/4CP) was 1.95 ± 0.04 mol of oxygen consumed per mol of 4CP removed. This YO2/4CP value reflected the action of the oxygenases responsible for the first steps of the aerobic oxidation of 4CP. The 4CP degradation activity decreased noticeably when successive pulses of 4CP were added to the respirometer. A mathematical model was developed to represent the aerobic biodegradation of 4CP. The fitted model adequately predicted the oxygen consumption rate, total phenols, and soluble COD concentrations as a function of time. The results presented could help to predict the dynamic of biodegradation of chlorophenols in a biological wastewater treatment system.

Keywords

Acclimated activated sludge 4-Chlorophenol Respirometry Biodegradation Oxidation coefficient Mathematical model Kinetics 

Notes

Acknowledgements

This work was supported by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), by Universidad Nacional de la Plata (UNLP), and by Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), Argentina.

Supplementary material

11356_2017_9735_MOESM1_ESM.docx (1 mb)
ESM 1 (DOCX 1064 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Cintia C. Lobo
    • 1
  • Nora C. Bertola
    • 1
  • Edgardo M. Contreras
    • 2
  • Noemí E. Zaritzky
    • 1
    • 3
  1. 1.Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), CONICET, Facultad de Ciencias, ExactasUNLPLa PlataArgentina
  2. 2.Instituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA), CONICET, Facultad de IngenieríaMar del PlataArgentina
  3. 3.Facultad de IngenieríaUNLPLa PlataArgentina

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