Monitoring and modeling 4-chlorophenol biodegradation kinetics by phenol-acclimated activated sludge by using open respirometry
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The aim of this study was to analyze the mechanisms, stoichiometry, and stability of 4-chlorophenol (4CP) biodegradation kinetics by phenol-acclimated activated sludge using open respirometry. While the removal of 4CP was higher than 98%, the removal of chemical oxygen demand (COD) ranged between 69 and 79% due to the accumulation of an intermediate metabolite. The value obtained from respirometric profiles for the stoichiometric ratio of O2 to 4CP (YO2/4CP) was 1.95 ± 0.04 mol of oxygen consumed per mol of 4CP removed. This YO2/4CP value reflected the action of the oxygenases responsible for the first steps of the aerobic oxidation of 4CP. The 4CP degradation activity decreased noticeably when successive pulses of 4CP were added to the respirometer. A mathematical model was developed to represent the aerobic biodegradation of 4CP. The fitted model adequately predicted the oxygen consumption rate, total phenols, and soluble COD concentrations as a function of time. The results presented could help to predict the dynamic of biodegradation of chlorophenols in a biological wastewater treatment system.
KeywordsAcclimated activated sludge 4-Chlorophenol Respirometry Biodegradation Oxidation coefficient Mathematical model Kinetics
This work was supported by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), by Universidad Nacional de la Plata (UNLP), and by Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), Argentina.
- Beltrán FJ (2004) Ozone reaction kinetics for water and wastewater systems. Lewis Publishers. CRC Press Co., Boca Raton, FloridaGoogle Scholar
- Frey PA, Hegeman AD (2007) Enzymatic reaction mechanisms. Oxford University Press, Inc. new YorkGoogle Scholar
- Gao J, Ellis LBM, Wackett LP (2010) The University of Minnesota biocatalysis/biodegradation database: improving public access. Nucleic Acids Res 38(Database issue):D488–D491. doi: 10.1093/nar/gkp771
- Greenberg AE, Clesceri LS, Eaton AD (1989) Standard methods for the examination of water and wastewater, 17th edn. American Public Health Association (APHA), Washington DCGoogle Scholar
- Grén I, Hupert-Kocurek K, Osiecka M, Guzik U, Wojcieszyńska D (2012) Toxicity of 4-chlorophenol under cometabolic conditions depending on the bacterial cell wall structure? Architecture, civil engineering. Environment (ACEE) 3:101–108Google Scholar
- Mendes P (1993) GEPASI: a software package for modelling the dynamics, steady states and control of biochemical and other systems computer applications in the biosciences: CABIOS 9:563-571Google Scholar
- Paca J, Kosteckova A, Pacova L, Prell A, Halecky M, Paca J Jr, Stiborova M, Kozliak E, Soccol CR (2010) Respirometry kinetics of phenol oxidation by Comamonas testosteroni Pb50 under various conditions of nutritional stress. Braz Arch Biol Technol 53(6):1519–1528. doi: 10.1590/S1516-89132010000600030 CrossRefGoogle Scholar
- Ros M (1993) Respirometry of Activated Sludge. Technomic Publishing Co., Inc. Basilea, SwitzerlandGoogle Scholar
- Rueda-Márquez JJ, Pintado-Herrera MG, Martín-Díaz ML, Acevedo-Merino A, Manzano MA (2015) Combined AOPs for potential wastewater reuse or safe discharge based on multi-barrier treatment (microfiltration-H2O2/UV-catalytic wet peroxide oxidation). Chem Eng J 270:80–90. doi: 10.1016/j.cej.2015.02.011 CrossRefGoogle Scholar
- Verweij W. Equilibria and constants in CHEAQS: selection criteria, sources and assumptions. Version 8 (April 2009). From: http://home.tiscali.nl/cheaqs/db_v8.pdf