Advertisement

Environmental Science and Pollution Research

, Volume 24, Issue 24, pp 20005–20014 | Cite as

Phosphorus uptake in four tree species under nitrogen addition in subtropical China

  • Juxiu LiuEmail author
  • Yiyong Li
  • Yue Xu
  • Shuange Liu
  • Wenjuan Huang
  • Xiong Fang
  • Guangcai Yin
Research Article
  • 262 Downloads

Abstract

Atmospheric N deposition is a serious problem in subtropical China where N is present in large amounts but P is deficient. Several studies hypothesized that N2 fixers can overcome phosphorus limitation by trading fixed N2 for soil phosphorus. In order to know whether N2 fixers could invest fixed N2 in extracellular phosphatase production and could stimulate arbuscular mycorrhizal fungi (AMF) to acquire soil P in N-rich subtropical China, an open-air greenhouse experiment was carried out. Two N2 fixers (Acacia mangium and Ormosia pinnata) and two non-N2 fixers (Schima superba and Pinus massoniana) were exposed to three levels of N addition: 5.6 kg ha−1 a−1 (ambient N), 15.6 kg ha−1 a−1 (middle N), and 20.6 kg ha−1 a−1 (high N). We found that the capacity of plants to acquire soil P in N-rich subtropical China is species specific. The higher P uptake rates were found for N2 fixers than non-N2 fixers under N addition, which were related to the greater soil APA and higher AMF (p < 0.01) in the soil of N2 fixers. However, with time, high N addition decreased more significant quantities of soil microbial phospholipid fatty acids (PLFAs) in the soil of N2 fixers than that of non-N2 fixers (p < 0.05). We conclude that N2 fixers have higher P uptake capacity than non-N2 fixers under ambient N deposition in subtropical China. However, continuing N deposition in the future might affect P uptake ability of N2 fixers as high N addition would decrease soil microbial PLFAs of N2 fixers.

Keywords

N addition P uptake N2 fixers Subtropical China 

Notes

Acknowledgments

This study was jointly funded by the National Natural Science Foundation of China (Grant Nos. 31570482, 31370530, and 31670487).

References

  1. Allison SD, Nielsen C, Hughes RF (2006) Elevated enzyme activities in soils under the invasive nitrogen-fixing tree Falcataria moluccana. Soil Biol Biochem 38:1537–1544CrossRefGoogle Scholar
  2. Anderson JM, Ingram J (1994) Tropical soil biology and fertility: a handbook of methods. Soil Sci 157:265CrossRefGoogle Scholar
  3. Balser T, Treseder K, Ekenler M (2005) Using lipid analysis and hyphal length to quantify AM and saprotrophic fungal abundance along a soil chronosequence. Soil Biol Biochem 37:601–604CrossRefGoogle Scholar
  4. Batterman SA, Wurzburger N, Hedin LO (2013) Nitrogen and phosphorus interact to control tropical symbiotic N2 fixation: a test in Inga punctata. J Ecol 101:1400–1408CrossRefGoogle Scholar
  5. Bossio D, Scow K, Gunapala N, Graham K (1998) Determinants of soil microbial communities: effects of agricultural management, season, and soil type on phospholipid fatty acid profiles. Microb Ecol 36:1–12CrossRefGoogle Scholar
  6. Chung H, Zak DR, Reich PB, Ellsworth DS (2007) Plant species richness, elevated CO2, and atmospheric nitrogen deposition alter soil microbial community composition and function. Glob Change Biol 13:980–989CrossRefGoogle Scholar
  7. Dieter D, Elsenbeer H, Turner BL (2010) Phosphorus fractionation in lowland tropical rainforest soils in central Panama. Catena 82:118–125CrossRefGoogle Scholar
  8. Dolling P (1995) Effect of lupins and location on soil acidification rates. Anim Prod Sci 35:753–763CrossRefGoogle Scholar
  9. Elser J, Fagan W, Kerkhoff A, Swenson N, Enquist B (2010) Biological stoichiometry of plant production: metabolism, scaling and ecological response to global change. New Phytol 186:593–608CrossRefGoogle Scholar
  10. Frossard E, Condron LM, Oberson A, Sinaj S, Fardeau J (2000) Processes governing phosphorus availability in temperate soils. J Environ Qual 29:15–23CrossRefGoogle Scholar
  11. Frostegård Å, Bååth E (1996) The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil. Biol Fertil Soils 22:59–65CrossRefGoogle Scholar
  12. Güsewell S (2004) N:P ratios in terrestrial plants: variation and functional significance. New Phytol 164:243–266CrossRefGoogle Scholar
  13. Hedin LO, Brookshire ENJ, Menge DNL, Barron AR (2009) Brookshire EJ, Menge DN, Barron AR. The nitrogen paradox in tropical forest ecosystems. Annu Rev Ecol Syst 40:613–635CrossRefGoogle Scholar
  14. Hedin LO (2004) Global organization of terrestrial plant–nutrient interactions. Proc Natl Acad Sci U S A 101:10849–10850CrossRefGoogle Scholar
  15. Houlton BZ, Wang YP, Vitousek PM, Field CB (2008) A unifying framework for dinitrogen fixation in the terrestrial biosphere. Nature 454:327–330CrossRefGoogle Scholar
  16. Huang W, Zhou G, Deng X, Liu J, Duan H, Zhang D (2015) Nitrogen and phosphorus productivities of five subtropical tree species in response to elevated CO2 and N addition. Eur J For Res 134:845–856CrossRefGoogle Scholar
  17. Huang W, Zhou G, Liu J (2012) Nitrogen and phosphorus status and their influence on aboveground production under increasing nitrogen deposition in three successional forests. Acta Oecol 44:20–27CrossRefGoogle Scholar
  18. Jia Y, Yu G, He N, Zhan X, Fang H, Sheng W (2014) Spatial and decadal variations in inorganic nitrogen wet deposition in China induced by human activity. Sci Rep-UK 4:3763. doi: 10.1038/srep03763 CrossRefGoogle Scholar
  19. Juma N, Tabatabai M (1988) Comparison of kinetic and thermodynamic parameters of phosphomonoesterases of soils and of corn and soybean roots. Soil Biol Biochem 20:533–539CrossRefGoogle Scholar
  20. Khaliq A, Sanders F (2000) Effects of vesicular–arbuscular mycorrhizal inoculation on the yield and phosphorus uptake of field-grown barley. Soil Biol Biochem 32:1691–1696CrossRefGoogle Scholar
  21. Kritzler UH, Johnson D (2010) Mineralisation of carbon and plant uptake of phosphorus from microbially-derived organic matter in response to 19 years simulated nitrogen deposition. Plant Soil 326:311–319CrossRefGoogle Scholar
  22. Liu G, Jiang N, Zhang L, Liu Z (1996) Soil physical and chemical analysis and description of soil profiles, vol 24. Chin Stand Meth Press, Beijing, p 266Google Scholar
  23. Liu JX, Zhou GY, Zhang DQ (2007) Simulated effects of acidic solutions on element dynamics in monsoon evergreen broad-leaved forest at Dinghushan, China. Part 1: dynamics of K, Na, Ca, Mg and P. Environ Sci Pollut Res 14:123–129CrossRefGoogle Scholar
  24. Liu J, Huang W, Zhou G, Zhang D, Liu S, Li Y (2013) Nitrogen to phosphorus ratios of tree species in response to elevated carbon dioxide and nitrogen addition in subtropical forests. Glob Chang Biol 19:208–216CrossRefGoogle Scholar
  25. Liu J, Zhang D, Zhou G, Faivre-Vuillin B, Deng Q, Wang C (2008) CO2 enrichment increases nutrient leaching from model forest ecosystems in subtropical China. Biogeosciences 5:1783–1795CrossRefGoogle Scholar
  26. Liu S, Li Y, Fang X, Huang W, Long F, Liu J (2015) Effects of the level and regime of nitrogen addition on seedling growth of four major tree species in subtropical China. Chin J Plant Ecol 39:950–961CrossRefGoogle Scholar
  27. Lu X, Mo J, Gilliam FS, Zhou G, Fang Y (2010) Effects of experimental nitrogen additions on plant diversity in an old-growth tropical forest. Glob Chang Biol 16:2688–2700CrossRefGoogle Scholar
  28. Marklein AR, Houlton BZ (2012) Nitrogen inputs accelerate phosphorus cycling rates across a wide variety of terrestrial ecosystems. New Phytol 193:696–704CrossRefGoogle Scholar
  29. Meason DF, Idol TW, Friday J, Scowcroft PG (2009) Effects of fertilisation on phosphorus pools in the volcanic soil of a managed tropical forest. For Ecol Manag 258:2199–2206CrossRefGoogle Scholar
  30. Mo J, Brown S, Xue J, Fang Y, Li Z (2006) Response of litter decomposition to simulated N deposition in disturbed, rehabilitated and mature forests in subtropical China. Plant Soil 282:135–151CrossRefGoogle Scholar
  31. Nasto MK, Alvarez-Clare S, Lekberg Y, Sullivan BW, Townsend AR, Cleveland CC (2014) Interactions among nitrogen fixation and soil phosphorus acquisition strategies in lowland tropical rain forests. Ecol Lett 17:1282–1289CrossRefGoogle Scholar
  32. Ortas I, Harris P, Rowell D (1996) Enhanced uptake of phosphorus by mycorrhizal sorghum plants as influenced by forms of nitrogen. Plant Soil 184:255–264CrossRefGoogle Scholar
  33. Quesada CA, Lloyd J, Schwarz M, Patino S, Baker TR, Czimczik C, Fyllas NM, Martinelli L, Nardoto GB, Schmerler J, Santos AJB, Hodnett MG, Herrera R, Luizao FJ, Arneth A, Lloyd G, Dezzeo N, Hilke I, Kuhlmann I, Raessler M, Brand WA, Geilmann H, Moraes JO, Carvalho FP, Araujo RN, Chaves JE, Cruz OF, Pimentel TP, Paiva R (2010) Variations in chemical and physical properties of Amazon forest soils in relation to their genesis. Biogeosciences 7:1515–1541CrossRefGoogle Scholar
  34. Reich PB, Oleksyn J (2004) Global patterns of plant leaf N and P in relation to temperature and latitude. Proc Natl Acad Sci U S A 101:11001–11006CrossRefGoogle Scholar
  35. Schneider K, Turrión MB, Gallardo JF (2000) Modified method for measuring acid phosphatase activities in forest soils with high organic matter content. Commun Soil Sci Plant Anal 31:3077–3088CrossRefGoogle Scholar
  36. Tabatabai M, Bremner J (1969) Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:301–307CrossRefGoogle Scholar
  37. Tang C, Unkovich MJ, Bowden JW (2010) Factors affecting soil acidification under legumes. III. Acid production by N-2-fixing legumes as influenced by nitrate supply. New Phytol 143:513–521CrossRefGoogle Scholar
  38. Townsend AR, Cleveland CC, Asner GP, Bustamante MM (2007) Controls over foliar N:P ratios in tropical rain forests. Ecology 88:107–118CrossRefGoogle Scholar
  39. Treseder KK (2008) Nitrogen additions and microbial biomass: a meta-analysis of ecosystem studies. Ecol Lett 11:1111–1120CrossRefGoogle Scholar
  40. Treseder KK, Vitousek PM (2001) Effects of soil nutrient availability on investment in acquisition of N and P in Hawaiian rain forests. Ecology 82:946–954CrossRefGoogle Scholar
  41. Turner BL, Engelbrecht BM (2011) Soil organic phosphorus in lowland tropical rain forests. Biogeochemistry 103:297–315CrossRefGoogle Scholar
  42. Vance CP, Uhde-Stone C, Allan DL (2003) Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. New Phytol 157:423–447CrossRefGoogle Scholar
  43. Vitousek PM, Howarth RW (1991) Nitrogen limitation on land and in the sea: how can it occur? Biogeochemistry 13:87–115CrossRefGoogle Scholar
  44. Waldrop MP, Zak DR, Sinsabaugh RL (2004) Microbial community response to nitrogen deposition in northern forest ecosystems. Soil Biol Biochem 36:1443–1451CrossRefGoogle Scholar
  45. Wang Q, Wang S, Liu Y (2008) Responses to N and P fertilization in a young Eucalyptus dunnii plantation: microbial properties, enzyme activities and dissolved organic matter. Appl Soil Ecol 40:484–490CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Juxiu Liu
    • 1
    Email author
  • Yiyong Li
    • 1
    • 2
  • Yue Xu
    • 1
  • Shuange Liu
    • 1
  • Wenjuan Huang
    • 1
  • Xiong Fang
    • 1
  • Guangcai Yin
    • 3
  1. 1.Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical GardenChinese Academy of SciencesGuangzhouChina
  2. 2.College of Forestry and Landscape ArchitectureAnhui Agricultural UniversityHefeiChina
  3. 3.Guangdong University of TechnologyGuangzhouChina

Personalised recommendations