Raw sugarcane bagasse as carbon source for xylanase production by Paenibacillus species: a potential degrader of agricultural wastes
- 205 Downloads
Paenibacillus species isolated from a variety of natural sources have shown to be important glycoside hydrolases producers. These enzymes play a key role in bio-refining applications, as they are central biocatalysts for the processing of different types of polymers from vegetal biomass. Xylanase production by three native isolates belonging to the genus Paenibacillus was approached by utilizing mineral-based medium and agricultural by-products as a convenient source to produce biocatalysts suitable for their degradation. While varieties of alkali pretreated sugarcane bagasse were useful substrates for the strains from Paenibacillus genus evaluated, raw sugarcane bagasse was the most effective substrate for endoxylanase production by Paenibacillus sp. AR247. This strain was then selected to further improvement of its enzyme production by means of a two-step statistical approach. It was determined that the carbon source, provided as an inexpensive agro-waste, as well as phosphate and magnesium were the culture media components that most influenced the enzyme production, which was improved three times compared to the screening results.
KeywordsPaenibacillus spp. Xylanase production Sugarcane bagasse Biorefinery
This work was supported by the National Scientific and Technical Research Council, Argentina, project ref. PIP-284 and by the National University of Tucumán, Argentina. E. DM. and P.M.S. thank CONICET for doctoral scholarships.
- Carvalho AF, Oliva-Neto P, Zaghetto de Almeida P, Bueno da Silva J, Escaramboni B, Pastore GM (2015) Screening of xylanolytic Aspergillus fumigatus for prebiotic xylooligosaccharide production using bagasse. Food Technol Biotechnol 53(4):428–435Google Scholar
- Padilha IQ, Valenzuela SV, Grisi TC, Díaz P, de Araújo DA, Pastor FI (2014) A glucuronoxylan-specific xylanase from a new Paenibacillus favisporus strain isolated from tropical soil of Brazil. Int Microbiol 17(3):175–184Google Scholar
- Teather RM, Wood PJ (1982) Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43(4):777–780Google Scholar
- Zheng HC, Sun MZ, Meng LC, Pei HS, Zhang XQ, Yan Z, Zeng WH, Zhang JS, Hu JR, Lu FP, Sun JS (2014) Purification and characterization of a thermostable xylanase from Paenibacillus sp. NF1 and its application in xylooligosaccharides production. J Microbiol Biotechnol 24(4):489–496CrossRefGoogle Scholar