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Environmental Science and Pollution Research

, Volume 24, Issue 23, pp 19057–19067 | Cite as

Raw sugarcane bagasse as carbon source for xylanase production by Paenibacillus species: a potential degrader of agricultural wastes

  • Enzo Di Marco
  • Pablo M. Soraire
  • Cintia M. Romero
  • Liliana B. Villegas
  • María Alejandra Martínez
Research Article

Abstract

Paenibacillus species isolated from a variety of natural sources have shown to be important glycoside hydrolases producers. These enzymes play a key role in bio-refining applications, as they are central biocatalysts for the processing of different types of polymers from vegetal biomass. Xylanase production by three native isolates belonging to the genus Paenibacillus was approached by utilizing mineral-based medium and agricultural by-products as a convenient source to produce biocatalysts suitable for their degradation. While varieties of alkali pretreated sugarcane bagasse were useful substrates for the strains from Paenibacillus genus evaluated, raw sugarcane bagasse was the most effective substrate for endoxylanase production by Paenibacillus sp. AR247. This strain was then selected to further improvement of its enzyme production by means of a two-step statistical approach. It was determined that the carbon source, provided as an inexpensive agro-waste, as well as phosphate and magnesium were the culture media components that most influenced the enzyme production, which was improved three times compared to the screening results.

Keywords

Paenibacillus spp. Xylanase production Sugarcane bagasse Biorefinery 

Notes

Acknowledgments

This work was supported by the National Scientific and Technical Research Council, Argentina, project ref. PIP-284 and by the National University of Tucumán, Argentina. E. DM. and P.M.S. thank CONICET for doctoral scholarships.

Supplementary material

11356_2017_9494_MOESM1_ESM.docx (16 kb)
ESM 1 (DOCX 15 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Enzo Di Marco
    • 1
  • Pablo M. Soraire
    • 1
  • Cintia M. Romero
    • 1
    • 2
  • Liliana B. Villegas
    • 3
  • María Alejandra Martínez
    • 1
    • 4
  1. 1.PROIMI (Planta Piloto de Procesos Industriales Microbiológicos), CONICETTucumánArgentina
  2. 2.Facultad de Bioquímica, Química y FarmaciaUniversidad Nacional de TucumánTucumánArgentina
  3. 3.INQUISAL (Instituto de Química San Luis), CONICET - Facultad de Química, Bioquímica y FarmaciaUniversidad Nacional de San LuisSan LuisArgentina
  4. 4.Facultad de Ciencias Exactas y TecnologíaUniversidad Nacional de TucumánTucumánArgentina

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