Genotoxicity in adult residents in mineral coal region—a cross-sectional study
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The present study assessed the DNA damage in environmentally exposed volunteers living in seven municipalities in an industrial coal region, through the use of the comet assay with blood cells and the micronucleus test with buccal cells. Blood and buccal smears were collected from 320 male volunteers living in seven cities inserted in a coal region. They were ages of 18 and 50 years and also completed a questionnaire intended to identify factors associated with DNA damage through a Poisson regression analysis. The comet assay detected significant differences in DNA damage in volunteers from different municipalities, and neighboring cities (Pedras Altas, Aceguá, and Hulha Negra) had a higher level of DNA damage in relation to control city. Some of the risk factors associated with identified DNA lesions included residence time and life habits. On the other hand, the micronucleus test did not identify differences between the cities studied, but the regression analysis identified risk factors such as age and life habits (consumption of mate tea and low carbohydrates diet). We conclude that there are differences in the DNA damage of volunteers from different cities of the carboniferous region, but the presence of micronuclei in the oral mucosa does not differ between the same cities. Furthermore, we alert that some related factors may increase the risk of genotoxicity, such as residence location and time, and living and food habits. Finally, we suggest the need for continuous biomonitoring of the population, as well as for investing in health promotion in these vulnerable populations.
KeywordsGenotoxicity Mutagenicity Environmental exposure Coal mining activities
Compliance of ethical standards
The study protocol was approved by the Research Ethics Committee in Health at the Universidade Federal do Rio Grande and approved with the opinion 036/2013 (CEPAS/FURG).
This study was funded by Companhia de Geração Térmica de Energia Elétrica CGTEE.
Conflict of interest
The authors declare that they have no conflict of interest.
Informed consent was obtained from all individual participants included in the study.
- Agência nacional de energia elétrica (2008) Atlas de Energia Elétrica no Brasil. http://www.aneel.gov.br. Acessed 26 May 2015
- Braga CF, Teixeira EC, Alves R (2004) Estudo de aerossóis atmosféricos e aplicação de modelos numéricos. Qumica Nov. 27(4):567–573Google Scholar
- Brasil (2012) Conselho Nacional de Saúde. Diretrizes e normas regulamentadoras sobre pesquisa envolvendo seres humanos. Resolução 466. CNS, BrasíliaGoogle Scholar
- Centro de ecologia (2000) Carvão e Meio Ambiente. UFGRS, Porto AlegreGoogle Scholar
- Da Silva-Júnior FMR, Baisch P, Vargas VMF, Muccillo-Baisch, AL (2010) Genetic damage caused by coal and its derivatives. In: James J. Stewart. (Org.). Coal Extraction. 1ed .Nova Publishers 2010 V: 29–48Google Scholar
- Dupont-Soares M, Muccillo-Baisch AL, Baisch PRM, Soares MC (2015) Intellectual capacity of children exposed to environmental pollution in the extreme south of Brazil. J Health Sci 3:183–195Google Scholar
- Düsman E, Berti AP, Soares LC, Vicentini VEP (2012) Principais Agentes Mutagênicos e Carcinógenos de Exposição Humana. SaBios: RevSaúde e Biol 7:66–81Google Scholar
- Ferigolo PC, Sagrillo MR (2013) Genotoxicidade relacionada ao consumo de chimarrão. Disciplinarum Scientia. Série: Ciências da Saúde 14(1):1–13Google Scholar
- International energy agency (2015) www.iea.org. Acessed 17 july 2015
- Kir T, Durmaz E, Ulutas OK, Cok I, Donbak L (2011) DNA hazard in furnace operating workers from a power plant. J Carcinogene Mutagene 2:4Google Scholar
- Lan Q, Mumford JL, Shen M, Demarini DM, Bonner MR, He X, Yeager M, Welch R, Chanock S, Tian L, Chapman RS, Zheng T, Keohavong P, Caporaso N, Rothman N (2004) Oxidative damage-related genes AKR1C3 and OGG1 modulate risks for lung cancer due to exposure to PAH-rich coal combustion emissions. Carcinogenesis 25:2177–2181CrossRefGoogle Scholar
- Li X, Yang Y, Xu X, Xu C, Hong J (2015) Air pollution from polycyclic aromatic hydrocarbons generated by human activities and their health effects in China. J Clean ProdGoogle Scholar
- Pereira AV, FDLM R, Oliveira AN, Tapety FI, Cavalcante AACM, Chaves TVS (2013b) Haematological and genotoxic profile study of workers exposed to medical waste. J Res Fundam Care Online 5(6):160–168Google Scholar
- Pires M, Querol X, Teixeira EC (2001) Caracterização do carvão de Candiota e de suas cinzas. Geochim Bras 15(2):113–130 in portuguese Google Scholar
- Rohr P, Kvitko K, Da Silva FR, Menezes APS, Porto C, Sarmento M, Decker N, Reyes JM, Allgayer MC, Furtado TC, Salvador M, Branco C, Da Silva J (2013b) Genetic and oxidative damage of peripheral blood lymphocytes in workers with occupational exposure to coal. Mutat Res 758:23–28CrossRefGoogle Scholar
- Tolbert PE, Shy CM, Allen JW (1992) Micronuclei and other nuclear anomalies in buccal smears: methods development. Mutat Res/Environ Mutagenesis Relat Sub 271(1):69–77Google Scholar