Microalgae have tremendous potential to grow rapidly, synthesize, and accumulate lipids, proteins, and carbohydrates. The effects of solvent extraction of lipids on other metabolites such as proteins and carbohydrates in lipid-extracted algal (LEA) biomass are crucial aspects of algal biorefinery approach. An effective and economically feasible algae-based oil industry will depend on the selection of suitable solvent/s for lipid extraction, which has minimal effect on metabolites in lipid-extracted algae. In current study, six solvent systems were employed to extract lipids from dry and wet biomass of Scenedesmus obliquus. To explore the biorefinery concept, dichloromethane/methanol (2:1 v/v) was a suitable solvent for dry biomass; it gave 18.75% lipids (dry cell weight) in whole algal biomass, 32.79% proteins, and 24.73% carbohydrates in LEA biomass. In the case of wet biomass, in order to exploit all three metabolites, isopropanol/hexane (2:1 v/v) is an appropriate solvent system which gave 7.8% lipids (dry cell weight) in whole algal biomass, 20.97% proteins, and 22.87% carbohydrates in LEA biomass.
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Ansari FA, Shriwastav A, Gupta SK, Rawat I, Guldhe A, Bux F (2015) Lipids extracted algae as a source for proteins and reduced sugar: a step closer to the biorefinery. Bioresour Technol 179:559–564
Araujo GS, Matos LJ, Fernandes JO, Cartaxo SJ, Goncalves LR, Fernandes FA, Farias WR (2013) Extraction of lipids from microalgae by ultrasound application: prospection of the optimal extraction method. Ultrason Sonochem 20(1):95–98
Balasubramanian S, Allen JD, Kanitkar A, Boldor D (2011) Oil extraction from Scenedesmus obliquus using a continuous microwave system—design, optimization, and quality characterization. Bioresour Technol 102:3396–3403
Balasubramanian RK, Yen Doan TT, Obbard JP (2013) Factors affecting cellular lipids extraction from marine microalgae. Chem Eng J 215-216:929–936
Becker EW (2007) Micro-algae as a source of proteins. Biotechnol Adv 25(2):207–210
Borowitzka MA (1997) Microalgae for aquaculture: opportunities and constraints. J Appl Phycol 9:393–401
Certik M, Shimizu S (1999) Biosíntesis and regulation of microbial polyunsaturated fatty acid production. J Biosci Bioeng 87:1–14
Chen IS, Shen SJ, Sheppard AJ (1981) Comparison of methylene chloride and chloroform for the extraction of fats from food products. Division of Nutrition and Drug Administration, Washington, DC 20204
Chen C-Y, Zhao X-Q, Yen H-W, Ho S-H, Cheng C-L, Lee D-J, Bai F-W, Chang J-S (2013) Microalgae-based carbohydrates for biofuel production. Biochem Eng J 78:1–10
Cheng J, Huang R, Li T, Zhou J, Cen K (2014) Biodiesel from wet microalgae: extraction with hexane after the microwave-assisted transesterification of lipids. Bioresour Technol 170:69–75
Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25(3):294–306
Choi C-H, Kim M-A, Hong S-W, Choi Y-S, Song Y-I, Kim S-H, Kim H-J (2010) Performance of a microbial fuel cell using a magnet attached cathode. Bull Kor Chem Soc 31(6):1729–1731
Christaki E, Florou-Paneri P, Bonos E (2011) Microalgae: a novel ingredient in nutrition. Int J Food Sci Nutr 62(8):794–799
Dai Y-M, Chen K-T, Chen C-C (2014) Study of the microwave lipid extraction from microalgae for biodiesel production. Chem Eng J 250:267–273
De Schamphelaire L, Verstraete W (2009) Revival of the biological sunlight-to-biogas energy conversion system. Biotechnol Bioeng 103(2):296–304
DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Analyt Chem 28:350–356
Guldhe A, Singh B, Rawat I, Ramluckan K, Bux F (2014) Efficacy of drying and cell disruption techniques on lipids recovery from microalgae for biodiesel production. Fuel 128:46–52
Halim R, Danquah MK, Webley PA (2012) Extraction of oil from microalgae for biodiesel production: a review. Biotechnol Adv 30(3):709–732
Ho SH, Huang SW, Chen CY, Hasunuma T, Kondo A, Chang JS (2013) Bioethanol production using carbohydrates-rich microalgae biomass as feedstock. Bioresour Technol 135:191–198
Ju ZY, Forster I, Dominy W (2009) Effects of supplementing two species of marine algae or their fractions to a formulated diet on growth, survival and composition of shrimp (Litopenaeus vannamei). Aquaculture 292:237–243
Ju ZY, Deng D-F, Dominy W (2012) A defatted microalgae (Haematococcus pluvialis) meal as a proteins ingredient to partially replace fishmeal in diets of Pacific white shrimp (Litopenaeus vannamei, Boone, 1931). Aquaculture 354-355:50–55
Kassim MA, Kirtania K, De La Cruz D, Cura N, Srivatsa SC, Bhattacharya S (2014) Thermogravimetric analysis and kinetic characterization of lipids-extracted Tetraselmis suecica and Chlorella sp. Algal Res 6:39–45
Kim Y-H, Park S, Kim MH, Choi Y-K, Yang Y-H, Kim HJ, Kim H, Kim H-S, Song K-G, Lee SH (2013) Ultrasound-assisted extraction of lipids from Chlorella vulgaris using [Bmim][MeSO4]. Biomass Bioenergy 56:99–103
Lam MK, Tan IS, Lee KT (2014) Utilizing lipids-extracted microalgae biomass residues for maltodextrin production. Chem Eng J235:224–230
Lardon L, Hélias A, Sialve B, Steyer J, Bernard O (2009) Life-cycle assessment of biodiesel production from microalgae. Environ Sci Technol 43(17):6475–6481
Lee JY, Yoo C, Jun SY, Ahn CY, Oh HM (2010) Comparison of several methods for effective lipids extraction from microalgae. Bioresour Technol 101:575–577
López CVG, García MDCC, Fernández FGA, Bustos CS, Chisti Y, Sevilla JMF (2010) Protein measurements of microalgal and cyanobacterial biomass. Bioresour Technol 101(19):7587–7591
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Proteins measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Miranda JR, Passarinho PC, Gouveia L (2012) Pre-treatment optimization of Scenedesmus obliquus microalga for bioethanol production. Bioresour Technol 104:342–348
Pragya N, Pandey KK, Sahoo PK (2013) A review on harvesting, oil extraction and biofuels production technologies from microalgae. Renew Sust Energy Rev 24:159–171
Prajapati SK, Malik A, Vijay VK (2014) Comparative evaluation of biomass production and bioenergy generation potential of Chlorella spp. through anaerobic digestion. Appl Energy 114:790–797
Ramluckan K, Moodley KG, Bux F (2014) An evaluation of the efficacy of using selected solvents for the extraction of lipids from algal biomass by the soxhlet extraction method. Fuel 116:103–108
Rashid N, Rehman MSU, Han J-I (2013) Recycling and reuse of spent microalgal biomass for sustainable biofuels. Biochem Eng J 75:101–107
Sathish A, Sims RC (2012) Biodiesel from mixed culture algae via a wet lipids extraction procedure. Bioresour Technol 118:643–647
Shin H-Y, Ryu J-H, Bae S-Y, Crofcheck C, Crocker M (2014) Lipid extraction from Scenedesmus sp. microalgae for biodiesel production using hot compressed hexane. Fuel 130:66–69
Taelman SE, De Meester S, Roef L, Michiels M, Dewulf J (2013) The environmental sustainability of microalgae as feed for aquaculture: a life cycle perspective. Bioresour Technol 150:513–522
Taher H, Al-Zuhair S, Al-Marzouqi AH, Haik Y, Farid M (2014) Effective extraction of microalgae lipids from wet biomass for biodiesel production. Biomass Bioenergy 66:159–167
Tanzi CD, Vian MA, Chemat F (2013) New procedure for extraction of algal lipids from wet biomass: a green clean and scalable process. Bioresour Technol 134:271–275
Wahidin S, Idris A, Shaleh SRM (2014) Rapid biodiesel production using wet microalgae via microwave irradiation. Energy Conver Manag 84:227–233
Wang X, Liu X, Wang G (2011) Two-stage hydrolysis of invasive algal feedstock for ethanol fermentation. J Integr Plant Biol 53(3):246–252
Yun Y, Cho S, Jung K, Kim M, Shin H, Kim D (2014) Inhibitory effect of chloroform on fermentative hydrogen and methane production from lipid-extracted microalgae. Int J Hydrog Energy 39:19256–19261
Yun Y-M, Sung S, Choi J-S, Kim D-H (2016) Two-stage co-fermentation of lipid-extracted microalgae waste with food waste leachate: a viable way to reduce the inhibitory effect of leftover organic solvent and recover additional energy. Int J Hydrog Energy 41:21721–21727
Zamalloa C, Boon N, Verstraete W (2012) Anaerobic digestibility of Scenedesmus obliquus and Phaeodactylum tricornutum under mesophilic and thermophilic conditions. Appl Energy 92:733–738
The authors hereby acknowledge the National Research Foundation and Durban University of Technology for providing financial assistance.
• Lipid yield in wet biomass was less than the dry biomass.
• Protein and carbohydrate yields were comparable in both dry and wet biomass.
• Solvents show varying efficiencies of extraction in wet and dry biomass.
• DCM/methanol (2:1 v/v) was suitable solvent for lipid extraction from dry biomass.
• Isopropanol/hexane (2:1 v/v) is appropriate solvent systems for wet biomass.
Responsible editor: Santiago V. Luis
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Ansari, F.A., Gupta, S.K., Shriwastav, A. et al. Evaluation of various solvent systems for lipid extraction from wet microalgal biomass and its effects on primary metabolites of lipid-extracted biomass. Environ Sci Pollut Res 24, 15299–15307 (2017). https://doi.org/10.1007/s11356-017-9040-3
- Primary metabolites
- Solvent extraction
- Wet biomass
- Scenedesmus obliquus