Interaction of TiO2 nanoparticles with proteins from aquatic organisms: the case of gill mucus from blue mussel
- 418 Downloads
To better understand the mechanisms of TiO2 nanoparticle (NP) uptake and toxicity in aquatic organisms, we investigated the interaction of NPs with the proteins found in gill mucus from blue mussels. Mucus is secreted by many aquatic organisms and is often their first line of defense against pathogens, xenobiotics, and other sources of environmental stress. Here, five TiO2 NPs and one SiO2 NP were incubated with gill mucus and run out on a one-dimensional polyacrylamide gel for a comparative qualitative analysis of the free proteins in the mucosal solution and the proteins bound to NPs. We then used nanoscale liquid chromatography coupled with tandem mass spectrometry to identify proteins of interest. Our data demonstrated dissimilar protein profiles between the crude mucosal solution and proteins adsorbed on NPs. In particular, extrapallial protein (EP), one of the most abundant mucus proteins, was absent from the adsorbed proteins. After thermal denaturation experiments, this absence was attributed to the EP content in aromatic amino acids that prevents protein unfolding and thus adsorption on the NP. Moreover, although the majority of the protein corona was qualitatively similar across the NPs tested here (SiO2 and TiO2), a few proteins in the corona showed a specific recruitment pattern according to the NP oxide (TiO2 vs SiO2) or crystal structure (anatase TiO2 vs rutile TiO2). Therefore, protein adsorption may vary with the type of NP.
KeywordsMussel Gill mucus Protein Adsorption TiO2 nanoparticles Interaction nanoLC-MS/MS 1D PAGE
The present study was supported by the Programme Transversal de Toxicologie at the CEA. We would like to thank Drs. Jean Labarre and Géraldine Klein for their assistance with the 1D gels.
- Allouni ZE, Gjerdet NR, Cimpan MR, Høl PJ (2015) The effect of blood protein adsorption on cellular uptake of anatase TiO2 nanoparticles. Int J Nanomedicine 10:687–695Google Scholar
- Canesi L, Ciacci C, Fabbri R, Balbi T, Salis A, Damonte G, Cortese K, Caratto V, Monopoli MP, Dawson K, Bergami E, Corsi I (2016) Interactions of cationic polystyrene nanoparticles with marine bivalve hemocytes in a physiological environment: role of soluble hemolymph proteins. Environ Res 150:73–81CrossRefGoogle Scholar
- Cassar L, Pepe C (2002) Hydraulic binder and cement compositions containing photocatalyst particles. Google PatentsGoogle Scholar
- Devineau S 2013: Protein adsorption on nanomaterials. Biochemistry and physical-chemistry of a new stress. Université Paris Sud - Paris XIGoogle Scholar
- Hu M, Lin D, Shang Y, Hu Y, Lu W, Huang X, Ning K, Chen Y, Wang Y (2017). CO2-induced pH reduction increases physiological toxicity of nano-TiO2 in the mussel Mytilus coruscus. Scientific Reports 7Google Scholar
- Klein G, Mathé C, Biola-Clier M, Devineau S, Drouineau E, Hatem E, Marichal L, Alonso B, Gaillard J-C, Lagniel G, Armengaud J, Carrière M, Chédin S, Boulard Y, Pin S, Renault J-P, Aude J-C, Labarre J (2016) RNA-binding proteins are a major target of silica nanoparticles in cell extracts. Nanotoxicology 10:1555–1564CrossRefGoogle Scholar
- Marucco A, Fenoglio I, Turci F, Fubini B (2013) Interaction of fibrinogen and albumin with titanium dioxide nanoparticles of different crystalline phases. J Phys Conf Ser 429Google Scholar
- OECD 2016: Nanomaterials in waste streams: current knowledge on risks and impacts. http://dx.doi.org/10.1787/9789264249752-en., OECD Publishing, Paris
- Tenzer S, Docter D, Rosfa S, Wlodarski A, Kuharev J, Rekik A, Knauer SK, Bantz C, Nawroth T, Bier C, Sirirattanapan J, Mann W, Treuel L, Zellner R, Maskos M, Schild H, Stauber RH (2011) Nanoparticle size is a critical physicochemical determinant of the human blood plasma corona: a comprehensive quantitative proteomic analysis. ACS Nano 5:7155–7167CrossRefGoogle Scholar
- Tenzer S, Docter D, Kuharev J, Musyanovych A, Fetz V, Hecht R, Schlenk F, Fischer D, Kiouptsi K, Reinhardt C, Landfester K, Schild H, Maskos M, Knauer SK, Stauber RH (2013) Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology. Nat Nanotechnol 8:772–781CrossRefGoogle Scholar