Environmental Science and Pollution Research

, Volume 24, Issue 10, pp 9702–9712 | Cite as

Protective effects of Eruca sativa (rocket) on abamectin insecticide toxicity in male albino rats

  • Noha M. MeligiEmail author
  • Hanaa Fawzy HassanEmail author
Research Article


The extensive use of insecticides has hazardous effects since they can damage human health as well as the environment. Abamectin has been widely used in veterinary medicine and as a pesticide in agriculture. It is considered as one of the most commonly used insecticides in Egypt. The focus of the present study is to examine the toxic effects of sublethal dose (1 mg/kg b.wt.) of abamectin (Crater 3.37% EC) in male albino rats and to evaluate the efficiency of Eruca sativa suspension to ameliorate the abamectin toxicity. The present study was achieved using 18 male albino rats. Rats were divided into three groups: normal control group, abamectin-treated group, and abamectin + E. sativa-treated group. Rats of the third group were orally administrated a mixture of sublethal dose of abamectin (1 mg/kg b.wt.) and E. sativa suspension (5 g/kg b.wt.) three times a week for 28 days. At the end of the study period, blood samples were collected from all groups to measure the various hematological and biochemical parameters. The results revealed that rats, after abamectin exposure, exhibited general signs of toxicity and disturbance in the hematological and biochemical parameters. In addition, administration of E. sativa suspension ameliorated the hematological and biochemical parameters. These findings suggested that the exposure to abamectin might be responsible for hypertension, liver and kidney dysfunction, lipid profile disturbance, and oxidative stress, reflected in hematological and biochemical parameters. It was also found that the administration of E. sativa suspension reduced the detrimental impact of abamectin on some hematological and biochemical parameters.


Eruca sativa Abamectin Albino rats Hematological parameters Biochemical parameters 


  1. Akhtar A, Deshmukh AA, Raut CG, Somkuwar AP, Bhagat SS (2012) Prallethrin induced serum biochemical changes in Wistar rats. Pestic Biochem Physiol 102:160–168CrossRefGoogle Scholar
  2. Alam MS, Kaur G, Jabbar Z, Javed K, Athar M (2006) Eruca sativa seed possess antioxidant activity and exert a protective effect induced on mercuric chloride induced renal toxicity. Food Chem Toxicol 45:910–920CrossRefGoogle Scholar
  3. Al-Okbi SY, Mohamed DA, Hamed TE, Esmail RSH, Donya SM (2014) Prevention of renal dysfunction by nutraceuticals prepared from oil rich plant foods. Asian Pac J Trop Biomed 4:618–627CrossRefGoogle Scholar
  4. Al-Qurainy F (2010) Application of inter simple sequence repeat (ISSR marker) to detect genotoxic effect of heavy metals on Eruca sativa (L.) Afr J Biotechnol 9:467–474Google Scholar
  5. Anac D, Martin-Prevel P (1999) Improved crop quality by nutrients management. Kluwer Academic Publisher, pp. 1–13Google Scholar
  6. Ansari MN, Ganaie MA (2014) Ameliorative effect of rocket leaves on fertility in streptozotocin-induced diabetic rats. Int Res J Biological Sci 3:89–97Google Scholar
  7. Badimon JJ, Badimon L, Fuester V (1990) Regression of atherosclerotic lesions by high-density lipoprotein plasma fraction in the cholesterol-fed rabbit. J Clin Invest 85:1234–1241CrossRefGoogle Scholar
  8. Bakhshi E, Maliki SA, Ahmed SS (2007) Estimation of nutritional value and trace elements content of Carthamus oxycantha, Eruca sativa and Plantago ovata. Pak J Bot 30:1181–1187Google Scholar
  9. Bergamini CM, Gambetti S, Dondi A, Cervellati C (2004) Oxygen, reactive oxygen species and tissue damage. Curr Pharm Des 10:1611–1626CrossRefGoogle Scholar
  10. Bergmeyer HU, Horder M, Rej R (1986) IFCC method for alanine aminotransferase (L-alanine: 2-oxoglutrate aminotransferase, EC J Clin Chem Clin Biochem 24:481–495Google Scholar
  11. Bhatti GK, Kiran R, Sandhir R (2010) Modulation of ethano-induced hepatotoxicity and oxidative stress by vitamin E supplementation in male Wistar rats. Pestic Biochem Physiol 98:26–32CrossRefGoogle Scholar
  12. Burtis CA, Ashwood ER (1994) Tietz textbook of clinical chemistry, 2nd edn. W. B. Saunders Company, PhiladelphiaGoogle Scholar
  13. Cartledge S, Lawson N (2000) Aldosterone and renin measurements. Ann Clin Biochem 37:262–278CrossRefGoogle Scholar
  14. Choudhari PD, Chakraharti CH (1984) Effect of acephate (Orthene), an organophosphorus insecticide, on lipid metabolism in albino rats. Ind J Exp Biol 22:45–49Google Scholar
  15. Choudhary N, Sharma M, Verma P, Joshi SC (2003) Hepato and nephrotoxicity in rat exposed to endosulfan. J Environ Biol 24:305–308Google Scholar
  16. Doumas BT, Bayse DD, Carter RJ, Peters T Jr, Schaffer R (1981) A candidate reference method for determination of total protein in serum. I. Development and validation. Clin Chem 27:1642–1650Google Scholar
  17. Eissa FI, Zidan NA (2010) Haematological, biochemical and histopathological alterations induced by abamectin and Bacillus thuringiensis in male albino rats. Acta Biol Hung 61:33–44CrossRefGoogle Scholar
  18. El-Gengaihi SE, Salem A, Bashandi SA, Ibrahim NA, Abd El Hamid SR (2004) Hypolipidemic effect of some vegetable oils in rats. Food Agric Environ 2:88–93Google Scholar
  19. El-Missiry MA, El Gindy AM (2000) Amelioration of alloxan induced diabetes mellitus and oxidative stress in rats by oil of Eruca sativa seeds. Ann Nutr Metab 44:97–100CrossRefGoogle Scholar
  20. El-Nattat WS, El-Kady RI (2007) Effect of different medicinal plant seeds residues on the nutritional and reproductive performance of adult male rabbits. Int J Agric Biol 9:479–485Google Scholar
  21. El-Shafey AAM, Seliem MME, El-Mahrouky F, Gabr WM, Kandil RA (2011) Some physiological and biochemical effects of oshar extract and abamectin biocide on male albino rats. J Am Sci 7:254–261Google Scholar
  22. El-Shenawy NS, El-Salmy F, Al-Eisa RA, El-Ahmary B (2010) Amelioratory effect of vitamin E on organophosphorus insecticide diazinon-induced oxidative stress in mice liver. Pes Biochem Physiol 96:101–107CrossRefGoogle Scholar
  23. Frankel EN, Kanner J, German JB, Parks E, Kinsella JE (1993) Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. Lancet 341:454–457CrossRefGoogle Scholar
  24. Gabr WM (2005) Potentiation effect of certain natural compounds to chlorophacinon toxicity against black rat, Rattus rattus. Egypt J Agric Res 84:1095–1102Google Scholar
  25. Gupta M, Mazumder UK, Kumar TS, Gomathi P, Kumar RS (2004) Antioxidant and hepatoprotective effects of Bauhinia racemosa against paracetamol and carbon tetrachloride induced liver damage in rats. Iranian J Pharmacol Ther 3:12–20Google Scholar
  26. Halliwell B, Gutteridge JMC (1999) Free radicals in biology and medicine, 3rd edn. Claredon Press, Oxford, pp 146–150Google Scholar
  27. Hassan GA, Salem MH, Abd-Allah GA, Shaker N, Abo-Elezz Z (1988) Effect of organophosphorous (dimethoate) and pyrethroid (decamethrin) pesticides on plasma levels of cortisol and thyroxine, and on some haematological characteristics in growing male rabbits. Indian J Anim Sci 58:1395–1401Google Scholar
  28. Hoe CM, Wilkinson JS (1973) Liver function: a review. Aust Vet J 49:163–169CrossRefGoogle Scholar
  29. Hsu DZ, Hsu CH, Huang BM, Liu MY (2001) Abamectin effects on aspartate aminotransferase and nitric oxide in rats. Toxicology 165:189–193CrossRefGoogle Scholar
  30. Hussein J, Salah A, Oraby F, El-Deen AN, El-Khayat Z (2010) Antihepatotoxic effect of Eruca sativa extracts on alcohol induced liver injury in rats. J Am Sci 6:381–389Google Scholar
  31. Ibrahim NA, El-Gamal BA (2003) Effect of diazinon, an organophosphate insecticide, on plasma lipid constituents in experimental animals. J Biochem Mol Biol 36:499–504Google Scholar
  32. Joshipura KJ, Hu FB, Manson JE, Stampfer MJ, Rimm EB, Speizer FE, Colditz G, Ascherio A, Rosner B, Spiegelman D, Willett WC (2001) The effect of fruit and vegetable intake on risk for coronary heart disease. Ann Intern Med 134:1106–1114CrossRefGoogle Scholar
  33. Kale M, Rathore N, John S, Bhatnagar D (1999) Lipid peroxidative damage on pyrethroid exposure and alterations in antioxidant status in rat erythrocytes: a possible involvement of reactive oxygen species. Toxicol Letters 102:197–205CrossRefGoogle Scholar
  34. Kaplan RM, Courtney CH, Kunkle WE, Zeng QY, Jernigan AD, Eagleson JS (1994) Efficacy of injectable abamectin against gastrointestinal tract nematodes and lungworms of cattle. Am J Vet Res 55(353):357Google Scholar
  35. Koller LD, Exon JH, Nixon JE (1979) Polycythemia produced in rats by environmental contaminants. Arch Environ Health 34:252–255CrossRefGoogle Scholar
  36. Lankas GR, Gordon LR (1989) Toxicology. P. 131–143. In: Campbell WC (ed) Ivermectin and abamectin. Springer-Verlag, New York, p 363Google Scholar
  37. Leonard PJ, Persaud J, Motwani R (1971) The estimation of plasma albumin by BCG RCG binding on the Technicon SMA 12/60 analyzer and a comparison with the HABA dye binding technique. Clin Chim Acta 35:409–412CrossRefGoogle Scholar
  38. Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469–474CrossRefGoogle Scholar
  39. McGill HC, McMahan CA, Kruski AW, Mott GE (1981) Relationship of lipoprotein cholesterol concentrations to experimental atherosclerosis in baboons. Arteriosclerosis 1:3–12CrossRefGoogle Scholar
  40. Michelangeli F, Robson MJ, East JM, Lee AG (1990) The conformation of pyrethroids bound to lipid bilayers. Biochim Biophys Acta 1028:49–57CrossRefGoogle Scholar
  41. Nussey G, Van Vuren JHJ, Du Preez HH (1995) Effect of copper on the differential white blood cell counts of the Mozambique tilapia (Oreochromis mossambicus). Comp Biochem Physiol 111C:381–388Google Scholar
  42. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358CrossRefGoogle Scholar
  43. Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidse. J Lab Clin Med 70:158–169Google Scholar
  44. Patton CJ, Crouch SR (1977) Spectrophotometric and kinetics investigation of the Berthelot reaction for the determination of ammonia. Anal Chem 49:464–469CrossRefGoogle Scholar
  45. Ponda MP, Hostetter TH (2006) Aldosterone antagonism in chronic kidney disease. Clin J Am Soc Nephrol 1:668–677CrossRefGoogle Scholar
  46. Ryhanen R, Herranen J, Karhonen K, Penttila I, Popvilanpi M, Puhakainen E (1984) Relationship between serum lipids, lipoproteins and pseudocholinesterase during organophosphate poisoning in rabbits. Int J Biochem 16:687–690CrossRefGoogle Scholar
  47. Saleh A (2010) Carbon tetrachloride-induced hepatotoxicity: protective effect of rocket Eruca sativa L. in rats. Am J Chin Med 38:7588Google Scholar
  48. Sarkar M, Sarkar K, Bhattacharjee R, Chatterjee M, Sil P (2005) Curative role of the aqueous extract of the herb, Phyllanthus niruri, against nimesulide induced oxidative stress in murine liver. Biomed Res 16:171–176Google Scholar
  49. Schaefer TJ, Wolford RW (2005) Disorders of potassium. Emerg Med Clin North America 23:723–747CrossRefGoogle Scholar
  50. Shakoori AR, Ali SS, Saleem MA (1988) Effects of six months feeding of cypermethrin on the blood and liver of albino rats. J Biochem Toxicol 3:59–72CrossRefGoogle Scholar
  51. Stein EA (1987) Lipids, lipoproteins and apolipoproteins. In: Tietz NW (ed) Fundamentals of clinical chemistry, 3rd edn. W. B. Saunders Company, Philadelphia, pp 478–479Google Scholar
  52. Thabrew MI, Joice PD, Rajatissa W (1987) Comparative study of the efficacy of Pavetta indica and Osbeckia octandra in the treatment of liver dysfunction. Planta Med 53:239–241CrossRefGoogle Scholar
  53. Walmsley RN, White GH (1994) A guide to diagnostic clinical chemistry, 3rd edn. Blackwell Scientific Publication, London, p 543Google Scholar
  54. Wattanapitayakul SK, Bauer JA (2001) Oxidative pathways in cardiovascular disease: roles, mechanisms, and therapeutic implications. Pharmacol Ther 89:187–206CrossRefGoogle Scholar
  55. Whealton A, Watson AJ, Rock RC (1994) Colorimetric determination of serum urea concentration. In: Burtis CA, Ashwood ER (eds) Tietz textbook of Clin. Chem. WB Saunders Company, London, pp 1528–1531Google Scholar
  56. You G, Smith CP, Kanai Y, Lee WA, Stelzner M, Hediger MA (1993) Cloning and characterization of the vasopressin-regulated urea transporter. Nature 365:844–847CrossRefGoogle Scholar
  57. Young DS, Friedman RB (2001) Effects of disease on clinical laboratory tests, vol 1 and 2, 4th edn. AACC Press, Washington, DCGoogle Scholar
  58. Zaahkouk SAM, Helal EGE, Abd-Rabo TEI, Rashed SZA (2000) Carbamate toxicity and protective effect of vit. A and vit. E. on some biochemical aspects of male albino rats. Egypt J Hosp Med 1:60–77Google Scholar
  59. Zilva JF, Pannall PR, Mayne PD (1988) Clinical chemistry in diagnosis and treatment. In: Arnold E (ed) Plasma enzymes in diagnosis, 5th edn. Hodder and Stoughton Limited, LondonGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Zoology Department, Faculty of ScienceMinia UniversityMiniaEgypt

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