Environmental Science and Pollution Research

, Volume 25, Issue 14, pp 13626–13632 | Cite as

Comparison of gold nanoparticles biosynthesized by cell-free extracts of Labrys, Trichosporon montevideense, and Aspergillus

  • Wenli Shen
  • Yuanyuan QuEmail author
  • Xuanying Li
  • Xiaofang Pei
  • Shengnan You
  • Qingxin Yin
  • Jingwei Wang
  • Qiao Ma
Research Article


Biosynthesis of gold nanoparticles (AuNPs) by microbes has received much attention as an efficient and eco-friendly process. However, the characteristics of AuNPs biosynthesized by different microbial cell-free extracts are rarely comparatively studied. In this study, three locally isolated strains, i.e., bacteria Labrys sp. WJW, yeast Trichosporon montevideense WIN, and filamentous fungus Aspergillus sp. WL-Au, were selected for AuNPs biosynthesis. UV-Vis absorption bands at 538, 539, and 543 nm confirmed the formation of AuNPs by these strains. Transmission electron microscopy and selected area electron diffraction analyses revealed that the as-synthesized AuNPs were crystalline with spherical or pseudo-spherical shapes. However, the average sizes of these AuNPs were diverse, which were 18.8, 22.2 and 9.5 nm, respectively. The biomolecules involved in nanoparticles stabilization were demonstrated by Fourier transform infrared spectroscopy analysis. Four common functional groups such as –N–H, –C=C, –N=O, and –S=O groups were detected in these AuNPs, while a distinct –C=O group was involved in WL-Au-AuNPs. The catalytic rate of WL-Au-AuNPs toward 4-nitrophenol reduction (0.37 min−1) was much higher than those of others (WJW-AuNPs 0.27 min−1 and WIN-AuNPs 0.23 min−1). This research would provide useful information for exploring efficient microbial candidates to synthesize AuNPs with excellent performances.


Gold nanoparticles Biosynthesis Bacterium Yeast Filamentous fungus 



This work was supported by the Program for New Century Excellent Talents in University (No. NCET-13-0077), the Fundamental Research Funds for the Central Universities (No. DUT14YQ107), and the Open Project of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (No. ESK201529).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Wenli Shen
    • 1
  • Yuanyuan Qu
    • 1
    Email author
  • Xuanying Li
    • 1
  • Xiaofang Pei
    • 1
  • Shengnan You
    • 1
  • Qingxin Yin
    • 1
  • Jingwei Wang
    • 1
  • Qiao Ma
    • 1
  1. 1.State Key Laboratory of Fine Chemicals, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and TechnologyDalian University of TechnologyDalianChina

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