Joint toxicity of six common heavy metals to Chlorella pyrenoidosa
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Six common heavy metals (Ni, Fe, Zn, Pb, Cd, and Cr) in the water environment were selected to present five groups of binary mixture systems (Ni-Fe, Ni-Zn, Ni-Pb, Ni-Cd, and Ni-Cr) through a direct equipartition ray design. Microplate toxicity analysis based on Chlorella pyrenoidosa measured the 96-h joint toxicities of the binary mixtures. Toxicity interaction of the binary mixture was analyzed by comparing the observed toxicity data with the reference model (concentration addition). The results indicated that Ni-Fe, Ni-Pb, and Ni-Cr mixtures showed additive effects at concentration tested. It was indicated that Ni-Zn and Ni-Cd mixtures presented additive effects at low concentrations whereas synergistic effects were seen at high concentrations.
KeywordsHeavy metal Joint toxicity Chlorella pyrenoidosa Concentration addition
The authors are especially grateful for the financial support received from the National Natural Science Foundation of China (21667013 and 21407032), Science Research and Technology Development Project of Guangxi (Guikehe1599005-2-2), and Special Funding for Guangxi “BaGui Scholar” Construction Projects.
Compliance with ethical standards
The authors declare that they have no conflict of interest.
- Chen CY, Yeh JT (1996) Toxicity of binary mixtures of reactive toxicants. Environ Toxicol Water Qual 11(2):83–90. https://doi.org/10.1002/(SICI)1098-2256(1996)11:2<83::AID-TOX2>3.0.CO;2-4 CrossRefGoogle Scholar
- Chen Q, Zhang J, Li XM, Liu L (2015a) Time-dependent microplate toxicity analysis (T-MTA) of several antibiotics to chlorella pyrenoidosa. Asian J Ecotoxicology 10(2):190–197Google Scholar
- Chen Q, Zhang J, Li XM et al (2015b) Time-dependent microplate toxicity analysis (T-MTA) of several antibiotics to chlorella pyrenoidosa [J]. Asian Journal of Ecotoxicology 10(2):190–197 (in Chinese)Google Scholar
- Fernandes AR, Mortimer DN, Rose MD, Knowles TG, White S, Gem M (2009) Occurrence of dioxins (PCDDs, PCDFs) and polychlorinated biphenyls (PCBs) in wild, farmed and processed fish, and shellfish. Food Addit Contam B Surveillance 2(1):15–20. https://doi.org/10.1080/02652030802654479 CrossRefGoogle Scholar
- Liu BQ, Ge HL, Liu SS (2006) Microplate luminometry for toxicity bioassay of environmental pollutant on a new type of fresh water luminescent bacterium (Vibrio-qinghaiensissp-Q67). Asian J Ecotoxicol 1:186–191Google Scholar
- Rahman MS, Molla AH, Saha N, Rahman A (2012) Study on heavy metals levels and its risk assessment in some edible fishes from Bangshi River, Savar, Dhaka, Bangladesh. Food Chem 134(4):1847–1854, Study on heavy metals levels and its risk assessment in some edible fishes from Bangshi River, Savar, Dhaka, Bangladesh. https://doi.org/10.1016/j.foodchem.2012.03.099 CrossRefGoogle Scholar
- Wang MC, Liu SS, Chen F (2014) Predicting the time-dependent toxicities of three triazine herbicide mixtures to V. qinghaiensis sp Q67 using the extended concentration addition model. Acta Chim Sin 72(1):55–60Google Scholar
- Yang GY, Wang LL, Lei XQ, Wang YJ, Li AF, Zhang CW (2014) Effects of lead and chromium on the growth, photosynthetic performance, and antioxidant activity of Scenedesmus obliquus. Acta Sci Circumst 34:1606–1614Google Scholar
- Yu XD, Shi Y, Zhou B, Wang QX, Zhang XX, Tang XX, Wang Y (2012) Acute toxic effects of heavy metal ions on growth of marine Bloom-Forming Microalgae. Res Environ Sci 25:1047–1053Google Scholar
- Yuan J, Liu SS, Wang LJ, Shao YM (2011) Optimization of microplate toxicity analysis method based on Chlorella pyrenoidosa. Res Environ Sci 24:553–558Google Scholar
- Zhou QX, Cheng Y, Zhang QR, Liang JD (2003) Quantitative relationship analysis of ecotoxicological effects of compound pollution. Science in China (Series C) 33(6):566–573Google Scholar