Environmental Science and Pollution Research

, Volume 25, Issue 23, pp 22429–22436 | Cite as

Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus

  • Qiongfang LiEmail author
  • Faqin Dong
  • Qunwei Dai
  • Cunkai Zhang
  • Lujia Yu
Interface Effect of Ultrafine Mineral Particles and Microorganisms


Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles. In the microscopic system, the microorganisms will affect the minerals through attachment, charge neutralization, and dissolution related to the cell surface structure and metabolite. To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain. Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties. High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS. The results revealed the EPS of B. mucitaginosus mainly consisted of protein and polysaccharides. Both the whole cell and its EPS could promote the dissolution of calcite particles into calcium ions. Due to the adhesion of organic groups on the calcite surface, the surface potential shifted significantly in the negative direction and the solution pH was clearly increased. The morphology of calcite surface was significantly changed after dissolution and re-crystallization. Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.


Bacteria EPS Calcite Fine particulate matter Surface properties Bacillus mucitaginosus 



This work was co-founded by the project of the National Natural Science Foundation of China (No. 41130746, 41472309) and a key project of the Sichuan Provincial Department of Education (No. 14ZA0093).


  1. Anbu P, Kang CH, Shin YJ, So JS (2016) Formations of calcium carbonate minerals by bacteria and its multiple applications. Spring 5(1):1–26CrossRefGoogle Scholar
  2. Basak BB, Biswas DR (2009) Influence of potassium solubilizing microorganism (Bacillus mucilaginosus) and waste mica on potassium uptake dynamics by sudan grass (Sorghum vulgare Pers.) grown under two Alfisols. Plant Soil 317:235–255CrossRefGoogle Scholar
  3. Borm PJ (2002) Particle toxicology: from coalmining to nanotechnology. Inhal Toxicol 14(3):311–324CrossRefGoogle Scholar
  4. Bennett PC, Melcer ME, Siegel DI, Hassett JP (1988) The dissolution of quartz in dilute aqueous solutions of organic acids at 25°C. Geochim Cosmochim Ac 52(6):1521–1530CrossRefGoogle Scholar
  5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefGoogle Scholar
  6. Chan YC, Simpson RW, Mctainsh GH, Vowles PD, Cohen DD, Bailey GM (1997) Characterization of chemical species in PM2.5 and PM10 aerosols in Brisbane Australia. Atmos Environ 31:2061–2080CrossRefGoogle Scholar
  7. Charlson RJ, Schwartz SE, Hales JM et al (1992) Climate forcing by anthropogenic aerosols. Science 255:423–430CrossRefGoogle Scholar
  8. Chen W (2014) Electric research about boundary membrane of minerals in northwest China fall dust and micro-organisms system. Southwest university of science and technology, China. (in Chinese)Google Scholar
  9. Comte S, Guibaud G, Baudu M (2008) Biosorption properties of extracellular polymeric substances (EPS) towards Cd, Cu and Pb for different pH values. J Hazard Mater 151:185–193CrossRefGoogle Scholar
  10. Dabek-Zlotorzynka E, Dann TF, Martinelango PK, Celo V, Brook JR, Mathieu D, Ding L, Austin CC (2011) Canadian National Air Pollution Surveillance (NAPS) PM2.5 speciation program: methodology and PM2.5 chemical composition for the years 2003-2008. Atmos Environ 45(3):673–686CrossRefGoogle Scholar
  11. Decho AW (1990) Microbial exopolymer secretions in ocean environments: their role(s) in food webs and marine processes. Oceanogr Mar Biol Ann Rev 28:73–153Google Scholar
  12. Deng H, Wang XM, Du C, Shen XC, Cui FZ (2012) Combined effect of ion concentration and functional groups on surface chemistry modulated CaCO3 crystallization. CrystEngComm 14(20):6647–6653CrossRefGoogle Scholar
  13. Devasia P, Natarajan KA, Sathyanarayana DN, Ramananda-Rao G (1993) Surface chemistry of thiobacillus ferrooxidans relevant to adhesion on mineral surfaces. Appl Environ Microb 59(12):4051–4055Google Scholar
  14. Dockery DW, Stone PH (2007) Cardiovascular risks from fine particulate air pollution. N Engl J Med 356:511–513CrossRefGoogle Scholar
  15. Domínguez L, Rodríguez M, Prats D (2010) Effect of different extraction methods on bound EPS from MBR sludges. Part I: Influence of extraction methods over three-dimensional EEM fluorescence spectroscopy fingerprint. Desalination 261(1-20):19–26Google Scholar
  16. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28(3):350–356CrossRefGoogle Scholar
  17. Dupraz C, Visscher PT, Baumgartner LK, Reid RP (2004) Microbe-mineral interactions: early carbonate precipitation in a hypersaline lake (Eleuthera Island, Bahamas). Sedimentology 51(4):745–765CrossRefGoogle Scholar
  18. Ehrlich HL (1996) How microbes influence mineral growth and dissolution. Chem Geol 132(1–4):5–9CrossRefGoogle Scholar
  19. Farahat M, Hirajima T, Sasaki K, Doi K (2009) Adhesion of Escherichia coli onto quartz, hematite and corundum: extended DLVO theory and flotation behavior. Colloids Surf B: Biointerfaces 74(1):140–149CrossRefGoogle Scholar
  20. Frølund B, Griebe T, Nielsen PH (1995) Enzymatic activity in the activated sludge floc matrix. Appl Microbiol Biotechnol 43(4):755–761CrossRefGoogle Scholar
  21. Ge LY, Deng HH, Gao HW, Wang HW (2010) Study on extraction of extracellular polymeric substances from activated. Environ Sci Manag(China) 35(9):47–50Google Scholar
  22. Guo PS, Xu J, LuoHW LWW, Li WH, Han-Qing Yu HQ, Xie Z, Wei SQ, Hu FC (2013) Thermodynamic analysis on the binding of heavy metals onto extracellular polymeric substances (EPS) of activated sludge. Water Res 47(2):607–614CrossRefGoogle Scholar
  23. Hayashi H, Tsuneda S, Hirata A, Sasaki H (2001) Soft particle analysis of bacterial cells and its interpretation of cell adhesion behaviors in terms of DLVO theory. Colloids Surf B: Biointerfaces 22(2):149–157CrossRefGoogle Scholar
  24. Ignatovaivanova T, Ivanov R, Iliev I, Ivanova I (2014) Study of anticorrosion effect of EPS from now strains Lactobacillus delbrueckii. Biotechnol Biotec Eq, 23(sup1):705-708Google Scholar
  25. Jia CY, Li PJ, Wei DZ, Zhang HR, Liu W (2010) Research advances on adsorption of bacteria to mineral surface. Microbiology China 37(4):607–613Google Scholar
  26. Kelebek H, Selli S, Canbas A, Cabaroglu T (2009) HPLC determination of organic acids, sugars, phenolic compositions and antioxidant capacity of orange juice and orange wine made from a Turkish cv. Kozan. Microchem J 91:187–192CrossRefGoogle Scholar
  27. Kinzlera K, Gehrkea T, Telegdib J, Sand W (2003) Bioleaching-a result of interfacial processes caused by extracellular polymeric substances(EPS). Hydrometallurgy 71:83–88CrossRefGoogle Scholar
  28. Lee BM, Shin HS, Hur J (2013) Comparison of the characteristics of extracellular polymeric substances for different extraction methods and sludge formation conditions. Chemosphere 90:237–244Google Scholar
  29. Leroy P, Devau N, Revil A, Bizi M (2013) Influence of surface conductivity on the apparent zeta potential of amorphous silica nanoparticles. J Colloid Interf Sci 410(22):81–93CrossRefGoogle Scholar
  30. Bin L, Ye C, Jing Z, Henry TH, Zhu L, Yuan S (2008) Microbial flocculation by silicate bacterium Bacillus mucilaginosus: applications and mechanisms. Bioresour Technol 99(11):4825–4831CrossRefGoogle Scholar
  31. López-Moreno A, Sepúlveda-Sánchez JD, Mercedes EM, Le BS (2014) Calcium carbonate precipitation by heterotrophic bacteria isolated from biofilms formed on deteriorated ignimbrite stones: influence of calcium on EPS production and biofilm formation by these isolates. Biofouling 30(5):547–560CrossRefGoogle Scholar
  32. Mo B, Lian B (2011) Interactions between Bacillus mucilaginosus and silicate minerals (weathered adamellite and feldspar): weathering rate, products, and reaction mechanisms. Chin J Geochem 30:187–192CrossRefGoogle Scholar
  33. Natarajan KA, Namita D (2001) Role of bacterial interaction and bioreagents in iron ore flotation. Int J Miner Process 62(1–4):143–157CrossRefGoogle Scholar
  34. Obst M, Dynes JJ, Lawrence JR, Swerhone GDW, Benzerara K (2009) Precipitation of amorphous CaCO3 (aragonite-like) by cyanobacteria: a STXM study of the influence of EPS on the nucleation process. Geochim Cosmochim Ac 73(14):4180–4198CrossRefGoogle Scholar
  35. Ou Y (2010) Study of effect of EPS on adhesion of A. ferrooxidans on chalcopyrite and pyrite mineral surface, central south university, China (in Chinese)Google Scholar
  36. Pogliani C, Donati E (1999) The role of exopolymers in the bioleaching of a non-ferrous metal Sulphide. J Ind Microbiol Biltech 22(2):88–92CrossRefGoogle Scholar
  37. Rudd T, Sterritt RM, Lester JN (1984) Complexation of heavy metals by extracellular polymers in the activated sludge process. J Water Pollut Control Fed 56(12):1260–1268Google Scholar
  38. Tourney J, Ngwenya BT (2009) Bacterial extracellular polymeric substances (EPS) mediate CaCO3 morphology and polymorphism. Chem Geol 262(3–4):138–146CrossRefGoogle Scholar
  39. Tsuneda S, Aikawa H, Hayashi H, Yuasa A, Hirata A, He JZ, Li CC (2003) Extracellular polymeric substances responsible for bacterial adhesion onto solid surface. FEMS Microbiol Lett 223(2):287–292CrossRefGoogle Scholar
  40. Vdovic N, Bigdan J (1998) Electrokinetics of natural and synthetic calcite suspensions. Colloids Surf A 137(1–3):7–14CrossRefGoogle Scholar
  41. Wang DJ, Zhou DM (2015) Biofilms and extracellular polymeric substances mediate the transport of graphene oxide nanoparticles in saturated porous media. J Hazard Mater 300:467–474CrossRefGoogle Scholar
  42. Welch SA, Barker WW, Banfield JF (1999) Microbial extracellular polysaccharides and plagioclase dissolution. Geochim Cosmochim Ac 63(9):1405–1419CrossRefGoogle Scholar
  43. Wexler AS, Ge Z (1998) Hydrophobic particles can activate at lower relative humidity than slightly hygroscopic ones: a Kohler theory incorporating surface fixed charge. J Geophys Res 103:6083–6088CrossRefGoogle Scholar
  44. Wright DT, Oren A (2005) Nonphotosynthetic bacteria and theformation of carbonates and evaporites through time. Geomicrobiol J 22(1):27–53CrossRefGoogle Scholar
  45. Wu T, Jun C, Bin L (2007) Advance in studies on the function of microbes to the weathering of silicate minerals. Bull Mineral Petrol Geochem 26:263–268 (in Chinese with English abstract)Google Scholar
  46. Xu P, SI S, Zhang YJ, Zhai YJ, Wei ZG (2016) Effect of extracellular polymeric substances(EPS)on anti-corrosion behavior of metals. Corrosion And Protection (China) 37(5):384–387Google Scholar
  47. Yang XX, Wei P, Feng LH (2013) Atmospheric particulate matter PM2.5 and its sources. Front Sci 7(26):12–19Google Scholar
  48. Zhang YM, Zhang XY, Sun JY, Hu GY, Shen XJ, Wang YQ, Wang TT, Wang DZ, Zhao Y (2014) Chemical composition and mass size distribution of PM1.0 at an elevated site in central east China. Atmos Chem Phys 14(10):15191–15218CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.College of Life Science and EngineeringSouthwest University of Science and TechnologyMianyangChina
  2. 2.Key Laboratory of Solid Waste Treatment and Resource RecycleMinistry of EducationMianyangChina
  3. 3.College of Environment and ResourcesSouthwest University of Science and TechnologyMianyangChina

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