Environmental Science and Pollution Research

, Volume 25, Issue 3, pp 2211–2230 | Cite as

Environmentally benign nanometric neem-laced urea emulsion for controlling mosquito population in environment

  • Prabhakar Mishra
  • Merlyn Keziah Samuel
  • Ruchishya Reddy
  • Brij Kishore Tyagi
  • Amitava Mukherjee
  • Natarajan ChandrasekaranEmail author
Research Article


The increasing risk of vector-borne diseases and the environmental pollution in the day-to-day life due to the usage of the conventional pesticides makes the role of nanotechnology to come into the action. The current study deals with one of the applications of nanotechnology through the formulation of neem urea nanoemulsion (NUNE). NUNE was formulated using neem oil, Tween 20, and urea using the microfluidization method. Prior to the development of nanoemulsion, the ratio of oil/surfactant/urea was optimized using the response surface modeling method. The mean droplet size of the nanoemulsion was found to be 19.3 ± 1.34 nm. The nanoemulsion was found to be stable for the period of 4 days in the field conditions which aids to its mosquitocidal activity. The nanoemulsion exhibited a potent ovicidal and larvicidal activity against A. aegypti and C. tritaeniorhynchus vectors. This result was corroborated with the histopathological analysis of the NUNE-treated larvae. Further, the effect of NUNE on the biochemical profile of the target host was assessed and was found to be efficacious compared to the bulk counterpart. The nanoemulsion was then checked for its biosafety towards the non-target species like plant beneficial bacterium (E. ludwigii), and phytotoxicity was assessed towards the paddy plant (O. sativa). Nanometric emulsion at the concentration used for the mosquitocidal application was found to be potentially safe towards the environment. Therefore, the nanometric neem-laced urea emulsion tends to be an efficient mosquito control agent with an environmentally benign property.


Response surface modeling High-pressure homogenization Neem urea nanoemulsion Larvicidal Ovicidal Enterobacter ludwigii Oryza sativa Aedes aegypti Culex tritaeniorhynchus 



We acknowledge Vellore Institute of Technology for providing the laboratory space and facilities. We deeply acknowledge ICMR VSF (VIR/RCI/AES/58/2014/ECD-1) for the financial aid provided by them. We also acknowledge Dr. K. Gopalarathinam, Senior Entomologist, ZET, Vellore, for his immense help in the identification of the mosquito species.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11356_2017_591_MOESM1_ESM.doc (196 kb)
ESM 1 (DOC 196 kb)


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Prabhakar Mishra
    • 1
  • Merlyn Keziah Samuel
    • 1
  • Ruchishya Reddy
    • 1
  • Brij Kishore Tyagi
    • 2
  • Amitava Mukherjee
    • 1
  • Natarajan Chandrasekaran
    • 1
    Email author
  1. 1.Centre for NanobiotechnologyVIT UniversityVelloreIndia
  2. 2.Department of Zoology & Environment SciencePunjabi UniversityPatialaIndia

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