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Neuroprotective effects of curcumin against acetamiprid-induced neurotoxicity and oxidative stress in the developing male rat cerebellum: biochemical, histological, and behavioral changes

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Abstract

Curcumin is a molecule found in turmeric root that has anti-inflammatory, antioxidant, and anti-tumor properties and has been widely used as both an herbal drug and a food additive to treat or prevent neurodegenerative diseases. This study aimed to investigate the effect of curcumin on neurobehavioral and neuropathological alterations induced by acetamiprid on male rats. Three groups of ten male Wistar rats each were used for the study: the first was a control group (CTR) that did not consume acetamiprid (ACE); the second was an experimental group (ACE) that consumed 40 mg/kg body weight/day of acetamiprid; and the third group (CUR) received curcumin (100 mg/kg) and acetamiprid (40 mg/kg) in combination. Neurobehavioral evaluations including inclined plane performance and forepaw grip time were studied. Treatment with CUR significantly prevented ACE-treated rats from impairments in the performance of neurobehavioral tests, indicating the presence of deficits on sensorimotor and neuromuscular responses. In addition, Curcumin administration protects rats against acetamiprid-induced cerebellum toxicity such as increase in AChE and BChE activities, decrease on cells viability, oxidative stress, and an increase of intracellular calcium. Taken together, these results demonstrate for the first time that ACE treatment substantially impairs the survival of primary neuronal cells through the induction of necrosis concomitantly with the generation of an oxidative stress. Additionally, curcumin reduced histopathological changes caused by ACE.

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Authors and Affiliations

  1. Laboratory of Venoms and Therapeutic Biomolecules, LR11IPT08, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis, Tunisia

    Ines Bini Dhouib

  2. Department of Biology, Faculty of Sciences of Tunis, Laboratory of Aggression Physiology and Endocrine Metabolic Studies, University of Tunis El Manar, Tunis, Tunisia

    Ines Bini Dhouib, Alya Annabi, Ines Rejeb, Yosra Dallagi, Yassin Bdiri, Mohamed Montassar Lasram & Saloua Fazaa

  3. Laboratory of Anatomo-Pathology, Institut Salah Azaiez, Tunis, Tunisia

    Raoudha Doghri

  4. Department of Biology, Faculty of Sciences of Tunis, Laboratory of Genetics, Immunology and Human Pathology, University of Tunis El Manar, Tunis, Tunisia

    Amel Elgaaied, Raja Marrakchi & Asma Gati

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  1. Ines Bini Dhouib
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  2. Alya Annabi
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  3. Raoudha Doghri
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  4. Ines Rejeb
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  7. Mohamed Montassar Lasram
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  8. Amel Elgaaied
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  9. Raja Marrakchi
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Correspondence to Ines Bini Dhouib.

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Responsible editor: Philippe Garrigues

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Dhouib, I.B., Annabi, A., Doghri, R. et al. Neuroprotective effects of curcumin against acetamiprid-induced neurotoxicity and oxidative stress in the developing male rat cerebellum: biochemical, histological, and behavioral changes. Environ Sci Pollut Res 24, 27515–27524 (2017). https://doi.org/10.1007/s11356-017-0331-5

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  • DOI: https://doi.org/10.1007/s11356-017-0331-5

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