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Environmental Science and Pollution Research

, Volume 24, Issue 34, pp 26763–26777 | Cite as

Optimisation of critical medium components and culture conditions for enhanced biomass and lipid production in the oleaginous diatom Navicula phyllepta: a statistical approach

  • Sanyo Sabu
  • Isaac Sarojini Bright Singh
  • Valsamma Joseph
Research Article

Abstract

Diatoms hold great promise as potential sources of biofuel production. In the present study, the biomass and lipid production in the marine diatom Navicula phyllepta, isolated from Cochin estuary, India and identified as a potential biodiesel feedstock, were optimized using Plackett-Burman (PB) statistical experimental design followed by central composite design (CCD) and response surface methodology (RSM). The growth analyses of the isolate in different nitrogen sources, salinities and five different enriched sea water media showed the best growth in the cheapest medium with minimum components using urea as nitrogen source at salinity between 25 and 40 g kg−1. Plackett-Burman experimental analyses for screening urea, sodium metasilicate, sodium dihydrogen phosphate, ferric chloride, salinity, temperature, pH and agitation influencing lipid and biomass production showed that silicate and temperature had a positive coefficient on biomass production, and temperature had a significant positive coefficient, while urea and phosphate showed a negative coefficient on lipid content. A 24 factorial central composite design (FCCD) was used to optimize the concentration of the factors selected. The optimized media resulted in 1.62-fold increase (64%) in biomass (1.2 ± 0.08 g L−1) and 1.2-fold increase (22%) in estimated total lipid production (0.11 ± 0.003 g L−1) compared to original media within 12 days of culturing. A significantly higher biomass and lipid production in the optimized medium demands further development of a two-stage strategy of biomass production followed by induction of high lipid production under nutrient limitation or varying culture conditions for large-scale production of biodiesel from the marine diatom.

Keywords

Diatom Navicula phyllepta Biodiesel Growth medium Plackett-Burman design Response surface methodology 

Notes

Acknowledgements

The authors acknowledge Dr. Sunitha Poulose and Dr. Sareen Sarah John for supporting analyses using Design Expert Software.

Funding information

The authors acknowledge the University Grants Commission, Government of India for the financial support under the major research grant (File No. 41-568/2012 (SR)),

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Sanyo Sabu
    • 1
  • Isaac Sarojini Bright Singh
    • 1
  • Valsamma Joseph
    • 1
  1. 1.National Centre for Aquatic Animal HealthCochin University of Science and TechnologyKochiIndia

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