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Influence of phosphorous fertilization on copper phytoextraction and antioxidant defenses in castor bean (Ricinus communis L.)

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Abstract

Application of fertilizers to supply appropriate nutrients has become an essential agricultural strategy for enhancing the efficiency of phytoremediation in heavy metal contaminated soils. The present study was conducted to investigate the beneficial effects of three types of phosphate fertilizers (i.e., oxalic acid-activated phosphate rock (APR), Ca(H2PO4)2, and NaH2PO4) in the range of 0–600 mg P kg−1 soil, on castor bean growth, antioxidants [antioxidative enzymes and glutathione (GSH)], and Cu uptake. Results showed that with the addition of phosphorus fertilizers, the dry weight of castor bean and the Cu concentration in roots increased significantly, resulting in increased Cu extraction. The phosphorus concentration in both shoots and roots was increased as compared with the control, and the Ca(H2PO4)2 treatment had the greatest effect. Application of APR, NaH2PO4, and Ca(H2PO4)2 reduced the malondialdehyde (MDA) content, and the activity of the two antioxidant enzymes superoxide dismustase (SOD, EC 1.15.1.1) and catalase (CAT, EC 1.11.1.6) in the leaves of castor bean. GSH concentration in leaves increased with the increasing levels of phosphorus applied to soil as well as the accumulation of phosphorus in shoots, compared to the control. These results demonstrated that the addition of phosphorus fertilizers can enhance the resistance of castor bean to Cu and increase the Cu extraction efficiency of the plant from contaminated soils.

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Acknowledgements

This study was financially supported by the National Natural Science Foundation of China (41371470) and the National Sci-Tech Support Plan (2015BAD05B02). The authors are grateful to acknowledge Professor Penny R. Hirsch, Department of Agroecology, Rothamsted Research (Harpenden, UK), for her helpful advices and careful modification.

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Correspondence to Hongqing Hu.

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Responsible editor: Roberto Terzano

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Huang, G., Rizwan, M.S., Ren, C. et al. Influence of phosphorous fertilization on copper phytoextraction and antioxidant defenses in castor bean (Ricinus communis L.). Environ Sci Pollut Res 25, 115–123 (2018). https://doi.org/10.1007/s11356-016-8132-9

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Keywords

  • Phosphorus fertilizer
  • Copper
  • Castor bean
  • Phytoextraction
  • Enhanced uptake
  • Antioxidant enzymes
  • Lipid peroxidation