Environmental Science and Pollution Research

, Volume 24, Issue 4, pp 3769–3779 | Cite as

Biosurfactant production by the crude oil degrading Stenotrophomonas sp. B-2: chemical characterization, biological activities and environmental applications

  • Boutheina Gargouri
  • María del Mar Contreras
  • Sonda Ammar
  • Antonio Segura-Carretero
  • Mohamed Bouaziz
Research Article
First Online:
Received:
Accepted:

Abstract

In this work, biosurfactant-producing microorganisms were isolated from hydrocarbon-contaminated water collected from Tunisian oilfield. After enrichment and isolation, different bacterial strains were preliminary studied for their biosurfactant/bioemulsifier properties when using crude oil as the unique carbon source. In particular, the isolate strain B-2, a Gram-negative, rod-shaped bacterium, efficiently emulsified crude oil. The extracellular biosurfactant product from this strain presented an emulsification activity above 70% and a hydrophobicity of 71%. In addition, a diameter of 6 cm was observed in the oil displacement test. The characterization of B-2 strain using 16S rDNA sequencing enables us to find a high degree of similarity with various members of the genus Stenotrophomonas (with a percentage of similarity of 99%). The emulsification activity of Stenotrophomonas biosurfactant B-2 was maintained in a wide range of pH (2 to 6), temperature (4 to 55 °C), and salinity (0 to 50 g L−1) conditions. It also enhanced the solubility of phenanthrene in water and could be used in the re-mobilization of hydrocarbon-contaminated environment. In addition, this biosurfactant exhibited antimicrobial and antioxidant properties. Infrared spectroscopy suggested potential lipidic and peptidic moieties, and mass spectrometry-based analyses showed that the biosurfactant contains mainly cyclic peptidic structures belonging to the class of diketopiperazines. Therefore, the B-2 strain is a promising biosurfactant-producing microorganism and its derived biosurfactant presents a wide range of industrial applications.

Keywords

Biosurfactant Bioremediation Diketopiperazine Emulsification Stenotrophomonas sp. 
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Notes

Acknowledgements

The authors would like to thank Dr. Sami Mnif from the Laboratory of Molecular and Cellular Screening Processes (Centre of Biotechnology of Sfax, University of Sfax, PO Box “1177”, 3018 Sfax, Tunisia) for the revision of the Biosurfactant section. The authors would like also to thank the “Ministère de l’Enseignement Supérieur et de la Recherche Scientifique” (Laboratory LR14ES08), Tunisia, for its financial support to this research work.

Supplementary material

11356_2016_8064_MOESM1_ESM.docx (531 kb)
ESM 1 (DOCX 530 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Boutheina Gargouri
    • 1
    • 2
  • María del Mar Contreras
    • 3
    • 4
    • 5
  • Sonda Ammar
    • 1
  • Antonio Segura-Carretero
    • 3
    • 4
  • Mohamed Bouaziz
    • 1
    • 2
  1. 1.Laboratoire d’Electrochimie et Environnement, Ecole Nationale d’Ingénieurs de SfaxUniversité de SfaxSfaxTunisia
  2. 2.Institut Supérieur de Biotechnologie de SfaxUniversité de SfaxSfaxTunisia
  3. 3.Research and Development Functional Food Centre (CIDAF)GranadaSpain
  4. 4.Department of Analytical Chemistry, Faculty of SciencesUniversity of GranadaGranadaSpain
  5. 5.Department of Analytical Chemistry, Campus of RabanalesUniversity of CórdobaCórdobaSpain

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