Environmental Science and Pollution Research

, Volume 25, Issue 1, pp 73–80 | Cite as

Effect of nanoparticle hydroxyapatite on the immobilization of Cu and Zn in polluted soil

  • Rui-Juan Sun
  • Jie-Hua Chen
  • Ting-Ting Fan
  • Dong-Mei Zhou
  • Yu-Jun WangEmail author
Research in soil pollution and remediation in China


Phosphate compounds and related materials are effective amendments for immobilization of heavy metals in contaminated soils. A greenhouse pot experiment with ryegrass (Lolium perenne) as the test plant was conducted to explore the impact of nanoparticle hydroxyapatite (HAP) on the immobilization and bioavailability of Cu and Zn in a heavy metal-polluted soil. The addition of nanoparticle HAP significantly decreased the uptake of Cu and Zn by ryegrass. As a result, the biomass of ryegrass increased as the rate of nanoparticle HAP increased. The toxicity characteristic leaching procedure (TCLP) and physiologically based extraction test (PBET) results of the treatments showed that the leachable and bioaccessible concentrations of Cu and Zn were significantly reduced after the soil stabilized with nanoparticle HAP. The XRD pattern of nanoparticle HAP was not changed by the presence of Cu and Zn, which suggests that Cu and Zn were immobilized by the formation of solid amorphous phosphate. Nanoparticle HAP was an effective material to immobilize heavy metals in contaminated soils.


Immobilization Bioavailability Heavy metals Nano-HAP TCLP PBET 



This research was supported by the National Natural Science Foundation of China (Grants No. 41422105) and the National Basic Research and Development Program (2013CB934303) and the Natural Science Foundation of Jiangsu Province (No. BK20130050).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Rui-Juan Sun
    • 1
  • Jie-Hua Chen
    • 1
  • Ting-Ting Fan
    • 1
  • Dong-Mei Zhou
    • 1
  • Yu-Jun Wang
    • 1
    Email author
  1. 1.Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil ScienceChinese Academy of SciencesNanjingChina

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