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Environmental Science and Pollution Research

, Volume 24, Issue 4, pp 3664–3674 | Cite as

Biotransformation of herbicides by aquatic microbial communities associated to submerged leaves

  • Louis CarlesEmail author
  • Florent Rossi
  • Muriel Joly
  • Pascale Besse-Hoggan
  • Isabelle Batisson
  • Joan Artigas
Research Article

Abstract

Leaf microbial communities possess a large panel of enzymes permitting the breakdown of leaf polymers as well as the transformation of organic xenobiotic compounds present in stream waters. This study aims to assess the potential of leaf microbial communities, exhibiting different exposure histories to pesticides (upstream versus downstream), to biotransform three maize herbicides (mesotrione, S-metolachlor, and nicosulfuron) in single and cocktail molecule exposures. The results showed a high dissipation of nicosulfuron (sulfonylurea herbicide) (from 29.1 ± 10.8% to 66 ± 16.2%, day 40) in both single and cocktail exposures, respectively, but not of mesotrione and S-metolachlor. The formation of nicosulfuron metabolites such as ASDM (2-(aminosulfonyl)-N,N-dimethyl-3-pyridinecarboxamide) and ADMP (2-amino-4,6-dimethoxypyrimidine) and the weak sorption (<0.4%) on the leaf matrix confirmed the transformation of this molecule by leaf microorganisms. In addition, the downstream communities showed a greater ability to transform nicosulfuron than the upstream communities suggesting that the exposure history to pesticides is an important parameter and can enhance the biotransformation potential of leaf microorganisms. After 40-day single exposure to nicosulfuron, the downstream communities were also those experiencing the greatest shifts in fungal and bacterial community diversity suggesting a potential adaptation of microorganisms to this herbicide. Our study emphasizes the importance of leaf microbial communities for herbicide biotransformation in polluted stream ecosystems where fungi could play a crucial role.

Keywords

Leaf-associated microorganisms Biodegradation Mesotrione Nicosulfuron S-metolachlor Herbicide mixture 

Notes

Acknowledgements

This project has been funded by the project EcoBioPest (Agence de l’Eau Loire-Bretagne), the Région Auvergne, the French Ministry for Higher Education and Research and the European Regional Development Fund.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11356_2016_8035_MOESM1_ESM.docx (2.6 mb)
Figure S1 (DOCX 2678 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Louis Carles
    • 1
    • 2
    • 3
    • 4
    Email author
  • Florent Rossi
    • 1
    • 2
  • Muriel Joly
    • 1
    • 2
  • Pascale Besse-Hoggan
    • 3
    • 4
  • Isabelle Batisson
    • 1
    • 2
  • Joan Artigas
    • 1
    • 2
  1. 1.CNRS, UMR 6023Laboratoire Microorganismes: Génome et Environnement (LMGE)AubièreFrance
  2. 2.Clermont Université, LMGEUniversité Blaise Pascal-Université d’AuvergneClermont-FerrandFrance
  3. 3.CNRS, UMR 6296Institut de Chimie de Clermont-Ferrand (ICCF)AubièreFrance
  4. 4.Clermont Université, Université Blaise PascalICCFClermont-FerrandFrance

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