Detection of comammox bacteria in full-scale wastewater treatment bioreactors using tag-454-pyrosequencing
- 1.3k Downloads
The nitrogen cycle has been expanded with the recent discovery of Nitrospira strains that can conduct complete ammonium oxidation (commamox). Their importance in the nitrogen cycle within engineered ecosystems has not yet been analyzed. In this research, the community structure of the Bacteria domain of six full-scale activated sludge systems and three autotrophic nitrogen removal systems in the Netherlands and China has been investigated through tag-454-pyrosequencing. The phylogenetic analyses conducted in the present study showed that just a few of the Nitrospira sequences found in the bioreactors were comammox. Multivariate redundancy analysis of nitrifying genera showed an outcompetition of Nitrosomonas and non-comammox Nitrospira. Operational data from the bioreactors suggested that comammox could be favored at low temperature, low nitrogen substrate, and high dissolved oxygen. The non-ubiquity and low relative abundance of comammox in full-scale bioreactors suggested that this phylotype is not very relevant in the nitrogen cycle in wastewater treatment plants.
KeywordsActivated sludge Comammox Nitrogen Phylogenetics Pyrosequencing
The authors would like to acknowledge the support given by the Department of Civil and Environmental Engineering of the University of Aalto, Finland; the Institute of Water Research of the University of Granada, Spain; and the Department of Biotechnology of the Technical University of Delft, the Netherlands; as well, the authors would like to acknowledge the support provided by the personnel of the wastewater treatment plants of Amsterdam West, Breda, Dokhaven, Harnaschpolder, Kortenoord, Vianen, Apeldoorn, Meihua East, and Olburgen.
Compliance with ethical standards
Conflicts of interest
The authors declare that they have no conflict of interest.
- Gonzalez-Martinez A, Poyatos JM, Hontoria E, Osorio F (2011) Treatment of effluents polluted by nitrogen with new biological technologies based on autotrophic nitrification-denitrification processes. 74–84Google Scholar
- Jordaan K, Bezuidenhout CC (2015) Bacterial community composition of an urban river in the North West Province, South Africa, in relation to physico-chemical water quality. Environ Sci Pollut Res 1–13. doi: 10.1007/s11356-015-5786-7
- Santoro AE (2016) The do-it-all nitrifier. Science 351:342–343. doi: 10.1126/science.add9671
- Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Bio Evol 30(12):2725–2729. doi: 10.1093/molbev/mst197