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Environmental Science and Pollution Research

, Volume 24, Issue 2, pp 1380–1388 | Cite as

Fe deficiency induced changes in rice (Oryza sativa L.) thylakoids

  • Yuwen Wang
  • Chao Xu
  • Kang Li
  • Xiaojie Cai
  • Min Wu
  • Guoxiang ChenEmail author
Research Article

Abstract

Iron deficiency is an important abiotic stress that limits productivity of crops all over the world. We selected a hybrid rice (Oryza sativa L.), LYPJ, which is super high-yield and widely cultured in China, to investigate changes in the components and structure of thylakoid membranes and photosynthetic performance in response to iron deficiency. Our results demonstrated that photosystem I (PSI) is the primary target for iron deficiency, while the changes in photosystem II (PSII) are important for rebuilding a balance in disrupted energy utilization and dissipation caused by differential degradation of photosynthetic components. The result of immunoblot analysis suggested that the core subunit PsaA declined drastically, while PsbA remained relatively stable. Furthermore, several organizational changes of the photosynthetic apparatus were found by BN-PAGE, including a marked decrease in the PSI core complexes, the Cytb 6 /f complex, and the trimeric form of the LHCII antenna, consistent with the observed unstacking grana. The fluorescence induction analysis indicated a descending PSII activity with energy dissipation enhanced markedly. In addition, we proposed that the crippled CO2 assimilation could be compensated by the enhanced of phosphoenolpyruvate carboxylase (PEPC), which is suggested by the decreased ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and photosynthetic efficiency.

Keywords

Thylakoid complexes Photosynthetic apparatus Rice Fe deficiency 

Abbreviations

BN

Blue native

Chl

Chlorophyll

DM

Dodecyl-β-d-maltoside

LHC

Light harvesting complex

PAGE

Polyacrylamide gel electrophoresis

PEPC

Phosphoenolpyruvate carboxylase

PSI

Photosystem I

PSII

Photosystem II

SDS

Sodium dodecyl sulfate

RuBisCO

Ribulose-1,5-bisphosphate carboxylase/oxygenase

Notes

Acknowledgments

This research was supported by the National Natural Science Foundation of China (No. 31271621), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and NSFC for Talents Training in Basic Science (J1103507, J1210025).

Authors’ contribution

This work was designed by G. Chen. Data analysis was performed by Y. Wang and M. Wu. Experiments were performed by K. Li and X. Cai. Y. Wang and C. Xu wrote the paper.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Yuwen Wang
    • 1
  • Chao Xu
    • 1
  • Kang Li
    • 1
  • Xiaojie Cai
    • 1
  • Min Wu
    • 1
    • 2
  • Guoxiang Chen
    • 1
    Email author
  1. 1.Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences CollegeNanjing Normal UniversityNanjingChina
  2. 2.Zijin CollegeNanjing University of Science and TechnologyNanjingChina

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