Cytotoxicity and oxidative stress responses of silica-coated iron oxide nanoparticles in CHSE-214 cells
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The present study aimed at investigating cytotoxicity and oxidative stress induced by silica-coated iron oxide nanoparticles functionalized with dithiocarbamate (Fe3O4 NPs) in Chinook salmon cells (CHSE-214) derived from Oncorhynchus tshawytscha embryos. A significant reduction in cell viability was evident in response to Fe3O4 NPs as revealed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay after 24 h of exposure. Out of the tested concentrations (10, 20, and 30 μg/ml), the highest concentration has shown significant decrease in the viability of cells after 24 h of exposure. Alterations in the morphology of CHSE-214 cells was also evident at 10 μg/ml concentration of Fe3O4 NPs after 24 h. Fe3O4 NPs elicited a significant dose-dependent reduction in total glutathione content (TGSH), catalase (CAT), glutathione reductase (GR) with a concomitant increase in lipid peroxidation (LPO), and protein carbonyl (PC) at highest concentration (30 μg/ml) after 24 h of exposure. In conclusion, our data demonstrated that Fe3O4 NPs have potential to induce cytotoxicity in CHSE-214 cells, which is likely to be mediated through reactive oxygen species generation and oxidative stress.
KeywordsChinook salmon Iron oxide nanoparticles Cytotoxicity Oxidative stress Lipid peroxidation Protein carbonyl
The authors are grateful to the Portuguese Foundation for Science and Technology (FCT) for post-doctoral grants to KS (SFRH/BPD/79490/2011) and to the Aveiro University Research Institute/CESAM.
- Ahne W (1985) Use of fish cell cultures for toxicity determination in order to reduce and replace the fish tests. Zentralbl Bakteriol Mikrobiol Hyg B 180:480–504Google Scholar
- Beutler E (1984) Red cell metabolism: a manual of biochemical methods. Second edition, Ernest Beutler. Grune & Stratton, New York, 1975, XVI, pp 160Google Scholar
- Bird RP, Draper AH (1984) Comparative studies on different methods of malondialdehyde determination. Methods Enzymol 105:299–305Google Scholar
- Bols N, Dayeh V, Lee L, Schirmer K (2005) Use of fish cell lines in the toxicology and ecotoxicology of fish. Piscine cell lines in environmental toxicology. Biochemistry and molecular biology of fishes 6: 43–84Google Scholar
- Carlberg I, Mannervik B (1975) Purification and characterization of the flavoenzyme glutathione reductase from rat liver. J Biol Chem 250:5475–5480Google Scholar
- Claiborne A (1985) Catalase activity. In: Greenwald RA (ed) Handbook of methods for oxygen radical research. CRC Press, Boca Raton, pp 283–284Google Scholar
- Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferases the first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139Google Scholar
- Magdolenova Z, Drlickova M, Henjum K, Rundén-Pran E, Tulinska J, Bilanicova D, Pojana G, Kazimirova A, Barancokova M, Kuricova M, Liskova A, Staruchova M, Ciampor F, Vavra I, Lorenzo Y, Collins A, Rinna A, Fjellsbø L, Volkovova K, Marcomini A, Amiry-Moghaddam M, Dusinska M (2015) Coating-dependent induction of cytotoxicity and genotoxicity of iron oxide nanoparticles. Nanotoxicology 9:44–56CrossRefGoogle Scholar
- Mori M, Wakabayashi M, Kaneko Y, Hasobe M (1998) Application of a suspension-cultured salmonid cell line CHSE-sp to cytotoxicity test. Fish Sci: FS 64:991–992Google Scholar
- Radu M, Cristina Munteanu M, Petrache S, Iren Serban A, Dinu D, Hermenean A, Sima C, Dinischiotu A (2010) Depletion of intracellular glutathione and increased lipid peroxidation mediate cytotoxicity of hematite nanoparticles in MRC-5 cells. Acta Biochim Pol 57:355Google Scholar
- Taju G, Abdul Majeed S, Nambi K, Sahul Hameed A (2014) In vitro assay for the toxicity of silver nanoparticles using heart and gill cell lines of Catla catla and gill cell line of Labeo rohita. Comp Biochem Physiol Part C: Toxicol & Pharmacol 161:41–52Google Scholar