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Environmental Science and Pollution Research

, Volume 24, Issue 1, pp 752–764 | Cite as

Analysis of the use of microcystin-contaminated water in the growth and nutritional quality of the root-vegetable, Daucus carota

  • J. Machado
  • J. Azevedo
  • M. Freitas
  • E. Pinto
  • A. Almeida
  • V. Vasconcelos
  • A. CamposEmail author
Research Article

Abstract

Toxic cyanobacterial blooms are often observed in freshwaters and may reflect the increased eutrophication of these environments and alterations in climate. Cyanotoxins, such as microcystins (MCs), are an effective threat to many life forms, ranging from plants to humans. Despite the research conducted to date on cyanotoxins, the risks associated to the use of contaminated water in agriculture require further elucidation. To tackle this aim, a research was conducted with the root-vegetable Daucus carota. The specific aims of this work were the following: (i) to evaluate the effects of MC-LR on the plant growth and photosynthesis; (ii) to evaluate the nutritional quality of carrot roots; and (iii) to measure bioaccumulation. To this purpose, young carrots were grown in soil during 1 month in natural conditions and exposed to Mycrocystis aeruginosa aqueous extracts containing environmentally realistic concentrations of MC-LR (10 and 50 MC-LR μg/L). The results showed that MC-LR may decrease root growth after 28 days of exposure to 50 μg/L and increase photosynthetic efficiency. We also observed changes in mineral and vitamin content in carrots as a result of the exposure to contaminated water. Moreover, MC-LR was detected in carrot roots by ELISA at very low concentration 5.23 ± 0.47 ng MC eq./g FW. The soil retained 52.7 % of the toxin potentially available for plants. This result could be attributed to MC-LR adsorption by soil particles or due to microbial degradation of the toxin. We conclude that the prolonged use of MC-LR-contaminated water may affect crop growth, alter the nutritional value of vegetable products, and potentiate contamination.

Keywords

Cyanobacteria Irrigation water Microcystin-LR Daucus carota Growth Vitamins Minerals 

Notes

Acknowledgments

This research was partially supported by the European Regional Development Fund (ERDF) through the COMPETE-Operational Competitiveness Programme and national funds through FCT-Foundation for Science and Technology under the project PEst-C/MAR/LA0015/2013 and by Porto University under the framework of the project IJUP2011_3. A. Campos work is supported by a post-doctoral grant (SFRH/BPD/103683/2014) from FCT.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

11356_2016_7822_MOESM1_ESM.pdf (150 kb)
ESM 1 (PDF 149 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • J. Machado
    • 1
  • J. Azevedo
    • 1
  • M. Freitas
    • 1
    • 3
  • E. Pinto
    • 2
    • 3
  • A. Almeida
    • 2
  • V. Vasconcelos
    • 1
    • 4
  • A. Campos
    • 1
    Email author
  1. 1.Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR)University of PortoPortoPortugal
  2. 2.REQUIMTE, Department of Chemical Sciences, Laboratory of Applied Chemistry, Faculty of PharmacyUniversity of PortoPortoPortugal
  3. 3.Department of Environmental Health of School of Allied Health Technologies, and CISA/Research Center in Environment and HealthPolytechnic Institute of PortoGaiaPortugal
  4. 4.Department of Biology, Faculty of SciencesUniversity of PortoPortoPortugal

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