Chlorella vulgaris was cultivated under limitation and starvation and under controlled conditions using different concentrations of nitrate (NaNO3) and phosphate (K2HPO4 and KH2PO4) chemicals in modified Bold basal medium (BBM). The biomass and lipid production responses to different media were examined in terms of optical density, cell density, dry biomass, and lipid productivity. In the 12-day batch culture period, the highest biomass productivity obtained was 72.083 mg L−1 day−1 under BBM - NcontrolPlimited condition. The highest lipid content, lipid concentration, and lipid productivity obtained were 53.202 %, 287.291 mg/L, and 23.449 mg L−1 day−1 under BBM - NControlPDeprivation condition, respectively. Nitrogen had a major effect in the biomass concentration of C. vulgaris, while no significant effect was found for phosphorus. Nitrogen and phosphorus starvation was found to be the strategy affecting the lipid accumulation and affected the lipid composition of C. vulgaris cultures.
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The work described in this paper was fully supported by a grant from the Research Grants Council of the Hong Kong Special Administrative Region, China (UGC/IDS16/14). Special thanks to Ms. Caroline Cottet for improving the manuscript.
Responsible editor: Philippe Garrigues
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Wong, Y., Ho, Y., Ho, K. et al. Maximization of cell growth and lipid production of freshwater microalga Chlorella vulgaris by enrichment technique for biodiesel production. Environ Sci Pollut Res 24, 9089–9101 (2017). https://doi.org/10.1007/s11356-016-7792-9
- Chlorella vulgaris
- Culture medium
- Algal cultivation
- Lipid production