Advertisement

Environmental Science and Pollution Research

, Volume 24, Issue 1, pp 527–538 | Cite as

Biotreatment of industrial olive washing water by synergetic association of microalgal-bacterial consortia in a photobioreactor

  • P. Maza-Márquez
  • A. González-Martínez
  • M. V. Martínez-Toledo
  • M. Fenice
  • A. Lasserrot
  • J. González-López
Research Article

Abstract

This study presents an effective technology for the olive processing industry to remediate olive washing water. A 14.5-L enclosed tubular photobioreactor was inoculated with a stable microalgal-bacterial consortium obtained by screening strains well adapted to olive washing water. The capacity of an enclosed tubular photobioreactor to remove toxic compounds was evaluated under photosynthesis conditions and without any external supply of oxygen. The results showed that the dominant green microalgae Scenedesmus obliquus, Chlorella vulgaris and the cyanobacteria Anabaena sp. and bacteria present in olive washing water (i.e. Pantoea agglomerans and Raoultella terrigena) formed a synergistic association that was resistant to toxic pollutants present in the effluent and during the initial biodegradation process, which resulted in the breakdown of the pollutant. Total phenolic compounds, COD, BOD5, turbidity and colour removals of 90.3 ± 11.4, 80.7 ± 9.7, 97.8 ± 12.7, 82.9 ± 8.4 and 83.3 ± 10.4 %, respectively, were recorded in the photobioreactor at 3 days of hydraulic retention time.

Graphical abstract

Biotreatment of industrial olive washing water by synergetic association of microalgal-bacterial consortia in a photobioreactor

Keywords

Photobioreactor (PBR) Olive washing water (OWW) Microalgal-bacterial consortium Synergistic relationships Non-metric multidimensional scaling (MDS) 

Notes

Acknowledgments

This research was supported by European Algatec project (FP7) SME/2008/1/232331. The Instituto de Parasitologia y Biologia Molecular Lopez Neyra (CSIC), Granada, Spain is acknowledged for their DNA sequencing services.

References

  1. APHA (2005) Standard methods for the examination of water and wastewater, 21st edn. American Public Health Association, Washington DCGoogle Scholar
  2. Babuponnusami A, Muthukumar K (2013) Treatment of phenol-containing wastewater by photoelectro-Fenton method using supported nanoscale zero-valent iron. Environ Sci Pollut Res 20:1596–1605CrossRefGoogle Scholar
  3. Bastos AER, Moon DH, Rossi A, Trevors JT, Tsai SM (2000) Salt-tolerant phenol-degrading microorganisms isolated form Amazonian soil samples. Arch Microbiol 174:346–352CrossRefGoogle Scholar
  4. Belaid C, Khadraoui M, Mseddi S, Kallel M, Elleuch B, Fauvarque JF (2013) Electrochemical treatment of olive mill wastewater: treatment extent and effluent phenolic compounds monitoring using some uncommon analytical tools. J Environ Sci 25:220–230CrossRefGoogle Scholar
  5. Bester MC, Jacobson D, Bauer FF (2012) Many Saccharomyces cerevisiae cell wall protein encoding genes are coregulated by Mss 11, but cellular adhesion phenotypes appear only flo protein dependent. G3 Genes Genom Geneti 2:131–141Google Scholar
  6. Borde X, Guieysse B, Delgado O, Muñoz R, Hatti-Kaul R, Nugier-Chauvin C, Patin H, Mattiasson B (2003) Synergistic relationships in algal-bacterial microcosms for the treatment of aromatic pollutants. Bioresour Technol 86:293–300CrossRefGoogle Scholar
  7. Busca G, Berardinelli S, Resini C, Arrighi L (2008) Technologies for the removal of phenol from fluid streets: a short review of recent developments. J Hazard Mater 160:265–288CrossRefGoogle Scholar
  8. Cerrone F, Barghini P, Pesciaroli C, Fenice M (2011) Efficient removal of pollutants from olive washing wastewater in bubble-column bioreactor by Trametes versicolor. Chemosphere 84:254–259CrossRefGoogle Scholar
  9. Chakraborty S, Bhattacharya T, Patel TN, Tiwari KK (2010) Biodegradation of phenol by native microorganisms isolated from coke processing wastewater. J Environ Biol 31:293–296Google Scholar
  10. Chedeville O, Debacq M, Porte C (2009) Removal of phenolic compounds present in olive mill wastewaters by ozonation. Desalination 249:865–869CrossRefGoogle Scholar
  11. Clarke K, Warwick R (2001) Change in marine communities: an approach to statistical analysis and interpretation. Plymouth Marine Laboratory, PlymouthGoogle Scholar
  12. Codd GA, Morrison LF, Mefcalf JS (2005) Cyanobacterial toxins: risk management for health protection. Tox and Applied Phar 203:264–272CrossRefGoogle Scholar
  13. D’Annibale A, Quaratino D, Federici F, Fenice M (2006) Effect of agitation and aeration on the reduction of pollutant load of olive mill wastewater by the white-rot fungus Panus tigrinus. Biochem Eng J 29:243–249CrossRefGoogle Scholar
  14. de Godos I, Blanco S, Garcia-Encina PA, Becares E, Muñoz R (2009) Long term operation of high rate algae ponds for the bioremediation of piggery wastewaters at high loading rates. Bioresour Technol 100:4332–4339CrossRefGoogle Scholar
  15. Delrue F, Alvarez-Diaz PD, Fon-Sing S, Fleury G, Sassi JF (2016) The environmental biorefinery: using microalgae to remediate wastewater, a win-win paradigm. Energies 9:1–19CrossRefGoogle Scholar
  16. Dhillon JK, Shivaraman N (1999) Biodegradation of organic and alkali cyanide compounds in a trickling filter. Indian. J Environ Prot 19:805–810Google Scholar
  17. Díez B, Pedrós-Alió C, Marsh TL, Massana R (2001) Application of denaturing gradient gel electrophoresis (DGGE) to study the diversity of marine picoeukaryotic assemblages and comparison of DGGE with other molecular techniques. Appl Environ Microbiol 67:2942–2951CrossRefGoogle Scholar
  18. El-Gohary F, Tawfik A (2009) Decolorization and COD reduction of disperse and reactive dyes wastewater using chemical-coagulation followed by sequential batch reactor (SBR) process. Desalination 249:1159–1164CrossRefGoogle Scholar
  19. Essam T, El Rakaiby M, Agha A (2014) Remediation of the effect of adding cyanides on an algal/bacterial treatment of a mixture of organic pollutants in a continuous photobioreactor. Biotechnol Lett 36:1773–1781CrossRefGoogle Scholar
  20. Ganzenko O, Huguenot D, van-Hullebusch ED, Esposito G, Oturan MA (2014) Electrochemical advanced oxidation and biological processes for wastewater treatment: a review of the combined approaches. Environ Sci Pollut Res 21:8493–8524CrossRefGoogle Scholar
  21. Gao F, Yang ZH, Li C, Wang YJ, Jin WH, Deng YB (2014) Concentrated microalgae cultivation in treated sewage by membrane photobioreactor operated in batch flow mode. Bioresour Technol 167:441–446CrossRefGoogle Scholar
  22. Ghasemi Y, Rasoul-Amini S, Fotooh-Abadi E (2011) Review: the biotransformation, biodegradation and bioremediation of organic compounds by microalgae. J Phycol 47:969–980CrossRefGoogle Scholar
  23. Gnanapragasam G, Senthilkumar M, Arutchelvan V, Velayutham T, Nagarajan S (2011) Bio-kinetic analysis on treatment of textile dye wastewater using anaerobic batch reactor. Bioresour Technol 102:627–632CrossRefGoogle Scholar
  24. González López CV, Acién Fernández FG, Fernández Sevilla JM, Sánchez Fernández JF, Cerón García MC, Molina Grima E (2009) Utilization of the cyanobacteria Anabaena sp. ATCC 33047 in CO2 removal processes. Bioresour Technol 100:5904–5910CrossRefGoogle Scholar
  25. González G, Herrera MG, García MT, Peña MIM (2001) Biodegradation of phenol in a continuous process: comparative study of stirred tank and fluidized-bed bioreactors. Bioresour Technol 76:245–251CrossRefGoogle Scholar
  26. Gonzalez C, Marciniak J, Villaverde S, Garcia-Encina PA, Munoz R (2008) Microalgae-based processes for the biodegradation of pretreated piggery wastewaters. Appl Microbiol Biotechnol 80:891–898CrossRefGoogle Scholar
  27. Guo XJ, Lu ZY, Wang P, Li H, Huang ZZ, Lin KF, Liu YD (2015) Diversity and degradation mechanism of an anaerobic bacterial community treating phenolic wastewater with sulfate as an electron acceptor. Environ Sci Pollut Res 22:16121–16132CrossRefGoogle Scholar
  28. Hussain A, Dubey SK, Kumar V (2015) Kinetic study for aerobic treatment of phenolic wastewater. Water Resourc Ind 11:81–90CrossRefGoogle Scholar
  29. International Olive Oil Council (IOOC) (2015) Available online: http://www.internationaloliveoil.org/. Accessed 1 June 2015
  30. Justino CI, Pereira R, Freitas AC, Rocha-Santos TA, Panteleitchouk TS, Duarte AC (2012) Olive oil mill wastewater before and after treatment: a critical review from the ecotoxicological point of view. Ecotoxicology 21:615–629CrossRefGoogle Scholar
  31. Komnitsas K, Zaharaki D (2012) Pre-treatment of olive mill wastewaters at laboratory and mill scale and subsequent use in agriculture: legislative framework and proposed soil quality indicators. Resour Conserv Recy 69:82–89CrossRefGoogle Scholar
  32. Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: Molecular Evolutionary Genetics Analysis Software. Arizona State University, TempeGoogle Scholar
  33. Lee YK (2001) Microalgal mass culture systems and methods: their limitation and potential. J Appl Phycol 13:307–315CrossRefGoogle Scholar
  34. Libra JA, Borchert M, Vigelahn L, Storm T (2004) Two stage biological treatment of a diazo reactive textile dye and the fate of the dye metabolites. Chemosphere 56:167–180CrossRefGoogle Scholar
  35. Liotta LF, Gruttadauria M, Di Carlo G, Perrini G, Librando V (2009) Heterogeneous catalytic degradation of phenolic substrates: catalysts activity. J Hazard Mater 162:588–606CrossRefGoogle Scholar
  36. Mahdavi H, Prasad V, Liu Y, Ulrich AC (2015) In situ biodegradation of naphthenic acids in oil sands tailings pond water using indigenous algae-bacteria consortium. Bioresour Technol 187:97–105CrossRefGoogle Scholar
  37. Markou G, Georgakakis D (2011) Cultivation of filamentous cyanobacteria (blue-green algae) in agro-industrial wastes and wastewaters: a review. Appl Energ 88:3389–3401CrossRefGoogle Scholar
  38. Maza-Márquez P, Martínez-Toledo MV, González-López J, Rodelas R, Juárez-Jiménez B, Fenice M (2013) Biodegradation of olive washing wastewater pollutants by highly efficient phenol-degrading strains selected from adapted bacterial community. Int Biodeter Biodegr 82:192–198CrossRefGoogle Scholar
  39. Maza-Márquez P, Martínez-Toledo MV, Fenice M, Andrade L, Laserrot A, González-López J (2014) Biotreatment of olive washing wastewater by a selected microalgal-bacterial consortium. Int Biodeter Biodegr 88:69–76CrossRefGoogle Scholar
  40. Mitra A, Mukhopadhyay S (2016) Biofilm mediated decontamination of pollutants from the environment. Bioengineering 3:44–59CrossRefGoogle Scholar
  41. Muñoz R, Guieysse B (2006) Algal-bacterial processes for the treatment of hazardous contaminants: a review. Water Res 40:2799–2815CrossRefGoogle Scholar
  42. Muñoz R, Jacinto M, Guieysse B, Mattiasson B (2005) Combined carbon and nitrogen removal from acetonitrile using algal-bacterial reactors. Appl Microbiol Biotechnol 67:699–707CrossRefGoogle Scholar
  43. Ochando-Pulido JM, Martinez-Ferez A (2015) Review on the recent use of membrane technology for olive mill wastewater purification. Membranes 5:513–531CrossRefGoogle Scholar
  44. Olguín E, Galicia S, Camacho R, Mercado G, Pérez T (1997) Production of Spirulina sp. in sea water supplemented with anaerobic effluents in outdoor raceway under temperature climatic conditions. Appl Microbiol Biotechnol 48:242–247CrossRefGoogle Scholar
  45. Ong SA, Uchiyama K, Inadama D, Ishida Y, Yamagiwa K (2010) Treatment of azo dye acid Orange 7 containing wastewater using up-flow constructed wetland with and without supplementary aeration. Bioresour Technol 101:9049–9057CrossRefGoogle Scholar
  46. Park Y, Je KW, Lee K, Jung SE, Choi TJ (2008) Growth promotion of Chlorella ellipsoidea by co-inoculation with Brevundimonas sp. isolated from the microalga. Hydrobiologia 598:219–228CrossRefGoogle Scholar
  47. Park J, Jin HF, Lim BR, Park KY, Lee K (2010) Ammonia removal from anaerobic digestion effluent of livestock waste using green alga Scenedesmus sp. Bioresour Technol 101:8649–8657CrossRefGoogle Scholar
  48. Pozo C, Rodelas B, Martinez-Toledo MV, Vilchez R, Gonzalez-Lopez J (2007) Removal of organic load from olive washing water by an aerated submerged biofilter and profiling of the bacterial community involved in the process. J Microbiol Biotechnol 17:784–791Google Scholar
  49. Ryu BG, Kim W, Nam K, Kim S, Lee B, Park MS (2015) A comprehensive study on algal-bacterial communities shift during thiocyanate degradation in a microalga mediated process. Bioresour Technol 191:496–504CrossRefGoogle Scholar
  50. Shi J, Podola B, Melkonian M (2014) Application of a prototype-scale twin-layer photobioreactor for effective N and P removal from different process stages of municipal wastewater by immobilized microalgae. Bioresour Technol 154:260–266CrossRefGoogle Scholar
  51. Singh A, Kumar V, Srivastana JN (2013) Assessment of bioremediation of oil and phenol contents in refinery waste water via bacterial consortium. Pet Environ Biotechnol 4:1–4Google Scholar
  52. Smith B, O’Neal G, Boyter H, Pisczek J (2007) Decolorizing textile dye wastewater by anoxic/aerobic treatment. J Chem Technol Biotechnol 82:16–24CrossRefGoogle Scholar
  53. Stainer RY, Kunisawa R, Mandael M, Cohen-Bazire G (1971) Purification and properties of unicellular blue-green algae (order Chroococcales. Bacteriol Rev 35:171–205Google Scholar
  54. Tambekar DH, Tale SD, Borkar PR (2013) Bioremediation of phenol by haloalkaliphilic microorganisms isolated from Lonar Lake. International Journal of Science, Environmental and. Technology 2:434–441Google Scholar
  55. Tamer E, Amin MA, Ossama ET, Bo M, Benoit G (2006) Biological treatment of industrial wastes in a photobioreactor. Water Sci Technol 53:117–125CrossRefGoogle Scholar
  56. Tang X, He LY, Tao XQ, Dang Z, Guo CL, GN L, Xi XY (2010) Construction of an artificial microalgal-bacterial consortium that efficiently degrades crude oil. J Hazard Mater 181:1158–1162CrossRefGoogle Scholar
  57. Tarlan E, Dilek FB, Yetis U (2002) Effectiveness of algae in the treatment of a wood-based pulp and paper industry wastewater. Bioresour Technol 84:1–5CrossRefGoogle Scholar
  58. Tchobanoglous G, Burton FL, Stensel HD (2003) Wastewater engineering: treatment and reuse, 4 edn. Metcalf & Eddy, McGraw-Hill, New YorkGoogle Scholar
  59. Van Den Hende S, Beelen V, Bore G, Boon N, Vervaeren H (2014) Up-scaling aquaculture wastewater treatment by microalgal bacterial flocs: from lab reactors to an outdoor raceway pond. Bioresour Technol 159:342–354CrossRefGoogle Scholar
  60. Vasconcelos-Fernandes T, Shrestha R, Sui Y, Papini G, Zeeman G, Vet LE, Wijffels RH, Lamers P (2015) Closing domestic nutrient cycles using microalgae. Environ Sci Technol 49:12450–12456CrossRefGoogle Scholar
  61. Wetherell DF (1961) Culture of fresh water algae in enriched natural seawater. Plant Physiol (Copenh) 14–16Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • P. Maza-Márquez
    • 1
    • 2
  • A. González-Martínez
    • 3
  • M. V. Martínez-Toledo
    • 1
  • M. Fenice
    • 4
  • A. Lasserrot
    • 5
  • J. González-López
    • 1
  1. 1.Department of Microbiology and Institute of Water ResearchUniversity of GranadaGranadaSpain
  2. 2.Departamento de MicrobiologíaFacultad de FarmaciaGranadaSpain
  3. 3.Department of Built Environment, School of EngineeringAalto UniversityEspooFinland
  4. 4.Dipartimento di Scienze Ecologiche e Biologiche (DEB)University of TusciaViterboItaly
  5. 5.Biotmicrogen S.L., Parque Tecnológico de Ciencias de la SaludGranadaSpain

Personalised recommendations