Hematological, biochemical, and toxicopathic effects of subchronic acetamiprid toxicity in Wistar rats
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Acetamiprid is one of the most widely used neonicotinoids. This study investigates toxic effects of repeated oral administration of three doses of acetamiprid (1/20, 1/10, and 1/5 of LD50) during 60 days. For this, male Wistar rats were divided into four different groups. Hematological, biochemical, and toxicopathic effects of acetamiprid were evaluated. According to the results, a significant decrease in the body weight gain at the highest dose 1/5 of LD50 of acetamiprid was noticed. An increase in the relative liver weight was also observed at this dose level. The hematological constituents were affected. A significant decrease in RBC, HGB, and HCT in rats treated with higher doses of acetamiprid (1/10 and 1/5 of LD50) was noted. However, a significant increase in WBC and PLT were observed at the same doses. Furthermore, acetamiprid induced liver toxicity measured by the increased activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphates (ALPs), and lactate dehydrogenase (LDH) which may be due to the loss of hepatic membrane architecture and hepatocellular damage. In addition, exposure to acetamiprid resulted in a significant decrease in the levels of superoxide dismutase and catalase activities (p ≤ 0.01) with concomitant increase in lipid peroxidation in rat liver. These findings highlight the subchronic hepatotoxicity of acetamiprid.
KeywordsAcetamiprid Hematological and biochemical parameters Hepatotoxicity Oxidative stress
This work was supported by funds allocated to the Research Unit of Histology and Genetic UR12ES10 by the “Ministère Tunisien de l’Enseignement Supérieur et de la Recherche Scientifique”. The authors thank the personnel of Laboratories of Biochemistry and Hematology, University Hospital of Monastir, for their help in determination of biochemical and hematological parameters.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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