Advertisement

Environmental Science and Pollution Research

, Volume 23, Issue 20, pp 20915–20921 | Cite as

Stoichiometry evaluation of biohydrogen production from various carbohydrates

  • Mohammad Mehdi Amin
  • Bijan Bina
  • Ensiyeh Taheri
  • Ali Fatehizadeh
  • Mohammad GhasemianEmail author
Research Article

Abstract

In this paper, biochemical hydrogen potential (BHP) tests were conducted to investigate H2 production from different substrate with acid-treated anaerobic digested sludge at the mesophilic range. The sludge was collected from an anaerobic digester and was subjected to sulfuric acid pretreatments at pH 3 for 24 h. The effects of substrate type (glucose, fructose, and sucrose as carbon source) were investigated in batch experiments. Results showed that substrate degradation rate for all of the substrates was up 95 % and the electron equivalent balance showed good closure for glucose and sucrose. Batch experiments showed that the maximum molar hydrogen yield with glucose, fructose, and sucrose was 3.27, 3.16, and 6.46 mol H2/mol of substrate. The maximum cumulative biohydrogen production was 1552, 1487, and 1366 mL and maximum hydrogen production rate was 308, 279, and 275 mL/h for glucose, sucrose, and fructose, respectively. The experimental results suggest that the formation of hydrogen associates with the main aqueous products, i.e., acetate butyrate.

Keywords

BHP Gompertz model Stoichiometry Substrate type 

Notes

Acknowledgment

This study was supported by the Isfahan University of medical Sciences Grant No. 193043.

References

  1. Adorno MAT, Hirasawa JS, Varesche MBA (2014) Development and validation of two methods to quantify volatile acids (C2-C6) by GC/FID: headspace (automatic and manual) and liquid-liquid extraction (LLE). Am J Anal Chem 5:406–414CrossRefGoogle Scholar
  2. Akutsu Y, Li Y-Y, Tandukar M, Kubota K, Harada H (2008) Effects of seed sludge on fermentative characteristics and microbial community structures in thermophilic hydrogen fermentation of starch. Int J Hydrog Energy 33:6541–6548CrossRefGoogle Scholar
  3. Amin MM, Zilles J, Greiner J, Charbonneau S, Raskin L, Morgenroth E (2006) Influence of the antibiotic erythromycin on anaerobic treatment of a pharmaceutical wastewater. Environ Sci Technol 40:3971–3977CrossRefGoogle Scholar
  4. Azwar MY, Hussain MA, Abdul-Wahab AK (2014) Development of biohydrogen production by photobiological, fermentation and electrochemical processes: a review. Renew Sust Energ Rev 31:158–173CrossRefGoogle Scholar
  5. Beckers L, Masset J, Hamilton C, Delvigne F, Toye D, Crine M, Thonart P, Hiligsmann S (2015) Investigation of the links between mass transfer conditions, dissolved hydrogen concentration and biohydrogen production by the pure strain Clostridium butyricum CWBI1009. Biochem Eng J 98:18–28CrossRefGoogle Scholar
  6. Chang S, Li JZ, Liu F (2011) Evaluation of different pretreatment methods for preparing hydrogen-producing seed inocula from waste activated sludge. Renew Energy 36:1517–1522CrossRefGoogle Scholar
  7. Dhar BR, Elbeshbishy E, Hafez H, Lee H-S (2015) Hydrogen production from sugar beet juice using an integrated biohydrogen process of dark fermentation and microbial electrolysis cell. Bioresour Technol 198:223–230CrossRefGoogle Scholar
  8. Dubois M, Gilles KA, Hamilton JK, Rebers P, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356CrossRefGoogle Scholar
  9. Ghosh D, Hallenbeck PC (2009) Fermentative hydrogen yields from different sugars by batch cultures of metabolically engineered Escherichia Coli DJT135. Int J Hydrog Energy 34:7979–7982CrossRefGoogle Scholar
  10. Ginkel SWV, Sung S (2001) Biohydrogen production as a function of pH and substrate concentration. Environ Sci Technol 35:4726–4730CrossRefGoogle Scholar
  11. Jones DT, Woods DR (1986) Acetone-butanol fermentation revisited. Microbiol Rev 50:484–524Google Scholar
  12. Lee H-S, Salerno MB, Rittmann BE (2008) Thermodynamic evaluation on H2 production in glucose fermentation. Environ Sci Technol 42:2401–2407CrossRefGoogle Scholar
  13. Lee H-S, Krajmalinik-Brown R, Zhang H, Rittmann BE (2009) An electron-flow model can predict complex redox reactions in mixed-culture fermentative BioH2: microbial ecology evidence. Biotechnol Bioeng 104:687–697Google Scholar
  14. Lee H-S, Vermaas WFJ, Rittmann BE (2010) Biological hydrogen production: prospects and challenges. Trends Biotechnol 28:262–271CrossRefGoogle Scholar
  15. Lee HS, Rittmann BE (2009) Evaluation of metabolism using stoichiometry in fermentative biohydrogen. Biotechnol Bioeng 102:749–758CrossRefGoogle Scholar
  16. Levin DB, Pitt L, Love M (2004) Biohydrogen production: prospects and limitations to practical application. Int J Hydrog Energy 29:173–185CrossRefGoogle Scholar
  17. Li Y-H, Bai Y-X, Pan C-M, Li W-W, Zheng H-Q, Zhang J-N, Fan Y-T, Hou H-W (2015) Effective conversion of maize straw wastes into bio-hydrogen by two-stage process integrating H2 fermentation and MECs. Environ Sci Pollut Res 22:18394–18403CrossRefGoogle Scholar
  18. Lin P-Y, Whang L-M, Y-R W, Ren W-J, Hsiao C-J, Li S-L, Chang J-S (2007) Biological hydrogen production of the genus Clostridium: metabolic study and mathematical model simulation. Int J Hydrog Energy 32:1728–1735CrossRefGoogle Scholar
  19. Liu I-C, Whang L-M, Ren W-J, Lin P-Y (2011) The effect of pH on the production of biohydrogen by clostridia: thermodynamic and metabolic considerations. Int J Hydrog Energy 36:439–449CrossRefGoogle Scholar
  20. Nasr N, Velayutham P, Elbeshbishy E, Nakhla G, El Naggar MH, Khafipour E, Derakhshani H, Levin DB, Hafez H (2015) Effect of headspace carbon dioxide sequestration on microbial biohydrogen communities. Int J Hydrog Energy 40:9966–9976CrossRefGoogle Scholar
  21. O-Thong S, Prasertsan P, Birkeland N-K (2009) Evaluation of methods for preparing hydrogen-producing seed inocula under thermophilic condition by process performance and microbial community analysis. Bioresour Technol 100:909–918CrossRefGoogle Scholar
  22. Rice EW, Bridgewater L, Association APH (2012) Standard methods for the examination of water and wastewater. American Public Health Association, Washington, DCGoogle Scholar
  23. Saady NMC, Chaganti SR, Lalman JA, Heath D (2012) Impact of culture source and linoleic acid (C18: 2) on biohydrogen production from glucose under mesophilic conditions. Int J Hydrog Energy 37:4036–4045CrossRefGoogle Scholar
  24. Simon K-MR, Lee HS, Lim JK, Kim TW, Lee J-H, Kang SG (2015) One-carbon substrate-based biohydrogen production: microbes, mechanism, and productivity. Biotechnol Adv 33:165–177CrossRefGoogle Scholar
  25. Singhal Y, Singh R (2014) Effect of microwave pretreatment of mixed culture on biohydrogen production from waste of sweet produced from Benincasa hispida. Int J Hydrog Energy 39:7534–7540CrossRefGoogle Scholar
  26. Tao Y, Chen Y, Wu Y, He Y, Zhou Z (2007) High hydrogen yield from a two-step process of dark- and photo-fermentation of sucrose. Int J Hydrog Energy 32:200–206CrossRefGoogle Scholar
  27. Wu K-J, Chang C-F, Chang J-S (2007) Simultaneous production of biohydrogen and bioethanol with fluidized-bed and packed-bed bioreactors containing immobilized anaerobic sludge. Process Biochem 42:1165–1171CrossRefGoogle Scholar
  28. Zhang Y, Liu G, Shen J (2005) Hydrogen production in batch culture of mixed bacteria with sucrose under different iron concentrations. Int J Hydrog Energy 30:855–860CrossRefGoogle Scholar
  29. Zhu H, Beland M (2006) Evaluation of alternative methods of preparing hydrogen producing seeds from digested wastewater sludge. Int J Hydrog Energy 31:1980–1988CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Mohammad Mehdi Amin
    • 1
    • 2
  • Bijan Bina
    • 1
    • 2
  • Ensiyeh Taheri
    • 2
    • 3
  • Ali Fatehizadeh
    • 2
    • 3
  • Mohammad Ghasemian
    • 1
    • 2
    Email author
  1. 1.Environment Research Center, Research Institute for Primordial Prevention of Non-communicable DiseaseIsfahan University of Medical SciencesIsfahanIran
  2. 2.Department of Environmental Health Engineering, School of HealthIsfahan University of Medical SciencesIsfahanIran
  3. 3.Student Research Center, School of HealthIsfahan University of Medical SciencesIsfahanIran

Personalised recommendations