Environmental Science and Pollution Research

, Volume 23, Issue 13, pp 13255–13267 | Cite as

Diversity of endophytic Pseudomonas in Halimione portulacoides from metal(loid)-polluted salt marshes

  • Jaqueline Rocha
  • Marta Tacão
  • Cátia Fidalgo
  • Artur Alves
  • Isabel Henriques
Research Article

Abstract

Phytoremediation assisted by bacteria is seen as a promising alternative to reduce metal contamination in the environment. The main goal of this study was to characterize endophytic Pseudomonas isolated from Halimione portulacoides, a metal-accumulator plant, in salt marshes contaminated with metal(loid)s. Phylogenetic analysis based on 16S rRNA and gyrB genes showed that isolates affiliated with P. sabulinigri (n = 16), P. koreensis (n = 10), P. simiae (n = 5), P. seleniipraecipitans (n = 2), P. guineae (n = 2), P. migulae (n = 1), P. fragi (n = 1), P. xanthomarina (n = 1), and Pseudomonas sp. (n = 1). Most of these species have never been described as endophytic. The majority of the isolates were resistant to three or more metal(loid)s. Antibiotic resistance was frequent among the isolates but most likely related to species-intrinsic features. Common acquired antibiotic resistance genes and integrons were not detected. Plasmids were detected in 43.6 % of the isolates. Isolates that affiliated with different species shared the same plasmid profile but attempts to transfer metal resistance to receptor strains were not successful. Phosphate solubilization and IAA production were the most prevalent plant growth promoting traits, and 20 % of the isolates showed activity against phytopathogenic bacteria. Most isolates produced four or more extracellular enzymes. Preliminary results showed that two selected isolates promote Arabidopsis thaliana root elongation. Results highlight the diversity of endophytic Pseudomonas in H. portulacoides from contaminated sites and their potential to assist phytoremediation by acting as plant growth promoters and as environmental detoxifiers.

Keywords

Pseudomonas Halimione portulacoides Endophytic Phytoremediation Plant growth promoters Metals 

Notes

Acknowledgments

This work was supported by European Funds (FEDER) through COMPETE and by National Funds through the Portuguese Foundation for Science and Technology (FCT) within project PhytoMarsh (PTDC/AAC-651 AMB/118873/2010-FCOMP-01-0124-FEDER-019328). Authors also acknowledge FCT financing to CESAM (UID/AMB/50017/2013) and iBiMED (UID/BIM/04501/2013), Artur Alves (FCT Investigator Programme–IF/00835/2013), Isabel Henriques (FCT Investigator Programme–IF/00492/2013) and Cátia Fidalgo (PhD grant SFRH/BD/85423/2012). The authors wish to thank Sofia Pereira and Paula Castro (Universidade Católica Portuguesa, Portugal) for providing positive controls for plant-growth promotion traits screening, Kornelia Smalla (Julius Kuhn Institut, Germany) for the phytopathogenic strains used in antimicrobial activity assays.

Supplementary material

11356_2016_6483_MOESM1_ESM.pdf (223 kb)
ESM 1(PDF 222 kb)

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© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Biology Department and CESAMUniversity of AveiroAveiroPortugal
  2. 2.Biology Department, CESAM and iBiMEDUniversity of AveiroAveiroPortugal

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