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Effects of glyphosate on hepatic tissue evaluating melanomacrophages and erythrocytes responses in neotropical anuran Leptodactylus latinasus

Abstract

Glyphosate (GLY) is the most used herbicide worldwide and its effects on anurans are well known. Pollutants can cause physiological and morphological effects. Therefore, this study evaluated the effects of GLY on hepatic melanomacrophages as a response to environmental stressors. Three treatments were exposed to different concentrations of pure GLY (100, 1000, and 10,000 μg g−1, respectively), and there was also a control group. After the experimental time, liver and blood were analyzed. Melanomacrophages (MMCs) were located between the hepatocyte cordons, close to sinusoids. GLY increased the melanin area in MMCs of Leptodactylus latinasus exposed since lowest concentration until highest concentration. GLY also changed the occurrence of hepatic catabolism pigments into melanomacrophages and erythrocyte nuclear abnormalities; therefore, it can interfere with the hepatic metabolism. In conclusion, GLY promotes alterations in the hepatic tissue and erythrocyte nuclear abnormalities. Furthermore, MMCs may be useful as morphological responses of GLY effects.

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Acknowledgments

We are grateful to Gabriela Baroni Leite for helping with the histological procedures, Dr. Fernando Ferrari for help in statistical analysis, and GLEBA S.A. for providing pure glyphosate. This study was supported by CONICET (PIP 0723), FAPESP (Process 2013/02067-5), and CNPq (Grant 306123/2012-6). JMPI was supported by Universidad Nacional de San Luis (Res. No. 109/11 CS), while LFB was supported by a post-doctoral fellowship (FAPESP 2014/00946-4).

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Correspondence to Classius de Oliveira.

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Histopathologic lesion in liver of L. latinasus after exposure of glyphosate. (XLSX 35 kb)

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Pérez-Iglesias, J.M., Franco-Belussi, L., Moreno, L. et al. Effects of glyphosate on hepatic tissue evaluating melanomacrophages and erythrocytes responses in neotropical anuran Leptodactylus latinasus . Environ Sci Pollut Res 23, 9852–9861 (2016). https://doi.org/10.1007/s11356-016-6153-z

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Keywords

  • Glyphosate
  • Melanomacrophages
  • Melanin
  • Hemosiderin
  • Morphological responses
  • Genotoxic effects