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Environmental Science and Pollution Research

, Volume 23, Issue 7, pp 6690–6699 | Cite as

Purification and identification of Bacillus subtilis SPB1 lipopeptide biosurfactant exhibiting antifungal activity against Rhizoctonia bataticola and Rhizoctonia solani

  • Inès MnifEmail author
  • Ariadna Grau-Campistany
  • Jonathan Coronel-León
  • Inès Hammami
  • Mohamed Ali Triki
  • Angeles Manresa
  • Dhouha Ghribi
Research Article

Abstract

This study reports the potential of a soil bacterium, Bacillus subtilis strain SPB1, to produce lipopeptide biosurfactants. Firstly, the crude lipopeptide mixture was tested for its inhibitory activity against phytopathogenic fungi. A minimal inhibitory concentration (MIC), an inhibitory concentration at 50 % (IC50 %), and an inhibitory concentration at 90 % (IC90 %) values were determined to be 0.04, 0.012, and 0.02 mg/ml, respectively, for Rhizoctonia bataticola with a fungistatic mode of action. For Rhizoctonia solani, a MIC, an IC50 %, and IC90 % values were determined to be 4, 0.25, and 3.3 mg/ml, respectively, with a fungicidal mode of action. For both of the fungi, a loss of sclerotial integrity, granulation and fragmentation of hyphal mycelia, followed by hyphal shriveling and cell lysis were observed with the treatment with SPB1 biosurfactant fraction. After extraction, separation, and purification, different lipopeptide compounds were identified in the culture filtrate of strain SPB1. Mass spectroscopic analysis confirmed the presence of different lipopeptide compounds consisting of surfactin isoforms with molecular weights of 1007, 1021, and 1035 Da; iturin isoforms with molecular weights of 1028, 1042, and 1056 Da; and fengycin isoforms with molecular weights of 1432 and 1446 Da. Two new clusters of lipopeptide isoforms with molecular weights of 1410 and 1424 Da and 973 and 987 Da, respectively, were also detected. This study reported the ability of a B. subtilis strain to co-produce lipopeptide isoforms with potential use as antifungal compounds.

Keywords

Lipopeptide Mass spectroscopy Surfactin, iturin, and fengycin Rhizoctonia sp. Antifungal 

Notes

Acknowledgments

This work has been supported by grants from “Tunisian Ministry of Higher Education, Scientific Research and Technology” and the “Tunisian Ministry of Agriculture.”

Compliance with ethical standards

Conflict of interest

No conflict of interest is declared.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Inès Mnif
    • 1
    • 2
    • 7
    Email author
  • Ariadna Grau-Campistany
    • 3
  • Jonathan Coronel-León
    • 4
  • Inès Hammami
    • 5
  • Mohamed Ali Triki
    • 6
  • Angeles Manresa
    • 4
  • Dhouha Ghribi
    • 1
    • 2
  1. 1.Unit “Enzymes and Bioconversion,” National School of Engineers of SfaxUniversity of SfaxSfaxTunisia
  2. 2.Higher Institute of Biotechnology of SfaxUniversity of SfaxSfaxTunisia
  3. 3.Faculty of Chemistry, Department of Organic ChemistryUniversity of BarcelonaBarcelonaSpain
  4. 4.Laboratory of Microbiology, Faculty of PharmacyUniversity of BarcelonaBarcelonaSpain
  5. 5.Higher School of Agriculture of KefKefTunisia
  6. 6.Laboratory “Amélioration et Protection des Ressources Génétiques de l’Olivier,” Institut de l’OlivierUniversity of SfaxSfaxTunisia
  7. 7.Inès Mnif, Unité “Enzyme et Bioconversion,” ENISSfaxTunisia

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