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Environmental Science and Pollution Research

, Volume 23, Issue 6, pp 5232–5242 | Cite as

The toxic effect of cypermethrin, amitraz and combinations of cypermethrin-amitraz in rats

  • Murat Kanbur
  • Yavuz Siliğ
  • Gökhan Eraslan
  • Mürsel Karabacak
  • Zeynep Soyer Sarıca
  • Serap Şahin
Research Article

Abstract

In this study, the effects of cypermethrin (CYP), amitraz (AMT) and combined cypermethrin-amitraz (CYP-AMT) on some serum biochemical, oxidative stress and drug-metabolising parameters were investigated in male Wistar albino rats. CYP, AMT and combined CYP-AMT were administered at doses of 80 mg kg−1 bw−1 of CYP and 170 mg kg−1 bw−1 of AMT for 1 day (single dose), and at doses of 12 mg kg−1 bw−1 of CYP and 25 mg kg−1 bw−1 of AMT for 40 days by oral gavage. Oxidative stress (malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and glucose-6-phosphate dehydrogenase (G6PD)), serum biochemical (glucose, triglyceride, cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), blood urea nitrogen (BUN), creatinine, asparatate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total protein, albumin) in blood/tissues (liver, kidney, brain, spleen and testis) and hepatic drug-metabolising (cytochrome P450 2E1 (CYP2E1), NADH-cytochrome b5 reductase (CYPb5), NADPH-cytochrome c reductase/NADPH cytocrome P450 reductase (CYTC), glutathione S-transferase (GST), glutathione (GSH)) parameters were measured in liver samples taken on days 1 and 40. In result, it was determined that CYP, AMT and their combinations led to significant changes in the parameters investigated, and it was ascertained that long-term exposure to insecticides and the administration of insecticide combinations produced greater toxic effects in comparison with the administration of insecticides alone.

Keywords

Toxicity Cypermethrin Amitraz Combination Rat 

Notes

Acknowledgement

This research (project code: TSA-091132) was supported by the Research Fund of Erciyes University.

Compliance with ethical standards

The authors declare that there are no conflicts of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Murat Kanbur
    • 1
  • Yavuz Siliğ
    • 2
  • Gökhan Eraslan
    • 1
  • Mürsel Karabacak
    • 3
  • Zeynep Soyer Sarıca
    • 4
  • Serap Şahin
    • 5
  1. 1.Faculty of Veterinary Medicine, Department of Pharmacology and ToxicologyErciyes UniversityKayseriTurkey
  2. 2.Faculty of Medicine, Department of BiochemistryCumhuriyet UniversitySivasTurkey
  3. 3.Safiye Çıkrıkcıoğlu Vacational Collage, Animal Health DepartmentErciyes UniversityKayseriTurkey
  4. 4.Hakan Çetinsaya Experimantal Animal CenterErciyes UniversityKayseriTurkey
  5. 5.Faculty of Pharmacy, Department of BiochemistryCumhuriyet UniversitySivasTurkey

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