Environmental Science and Pollution Research

, Volume 23, Issue 4, pp 3727–3735 | Cite as

Degradation of monocrotophos by Starkeya novella YW6 isolated from paddy soil

  • Lina Sun
  • Shijun Zhu
  • Zhengzhong Yang
  • Qing Chen
  • Hongming Liu
  • Jun Zhang
  • Gang Hu
  • Shunpeng Li
  • Qing Hong
Research Article
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Abstract

A bacteria strain, YW6, capable of utilizing monocrotophos (MCP) as the sole carbon and nitrogen sources for growth was isolated from paddy soil and identified as Starkeya novella. Strain YW6 completely degraded 0.2 mM MCP within 36 h without any lag period. Addition of carbon source resulted in slowing down of the initial rate of degradation of MCP, while the presence of a more favorable source of nitrogen enhanced the degradation of MCP. In addition to the degradation of MCP, strain YW6 was also able to degrade a wide range of organophosphorus pesticides (OPs) containing P–O–C bond, but not dimethoate, which has P–S–C bond. A MCP degradation pathway was proposed on the basis of metabolite production patterns and identification of the metabolites. MCP is hydrolyzed at the P–O–C bond to form N-methylacetoacetamide and dimethyl phosphate; N-methylacetoacetamide is transformed to N-methyl-4-oxo-pentanamide, which was subsequently converted to 5-(methylamino)-5-oxo-pentanoic acid, and 5-(methylamino)-5-oxo-pentanoic acid is cleaved to glutaric acid and methylamine. These findings provide new insights into the microbial metabolism of MCP. To the best of our knowledge, this is the first report on the degradation of MCP by Starkeya bacteria.

Keywords

Monocrotophos MCP Starkeya novella YW6 Degradation pathway Organophosphorus pesticide Metabolite 
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Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (31370155, J1210056), the Project for Science and Technology of Jiangsu province (BE2012749), the Jiangsu Postdoctoral Science Foundation (1102079C), and the Jiangsu Agriculture Science and Technology Innovation Fund (CX (15)1004).

Supplementary material

11356_2015_5606_MOESM3_ESM.docx (659 kb)
ESM 1 (DOCX 659 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Lina Sun
    • 1
  • Shijun Zhu
    • 1
  • Zhengzhong Yang
    • 1
  • Qing Chen
    • 1
  • Hongming Liu
    • 1
  • Jun Zhang
    • 2
  • Gang Hu
    • 1
  • Shunpeng Li
    • 1
  • Qing Hong
    • 1
  1. 1.Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life SciencesNanjing Agricultural UniversityNanjingChina
  2. 2.College of Resources and Environmental SciencesNanjing Agricultural UniversityNanjingChina

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