To enrich the understanding on interactions between carbon nanotubes (CNTs) and microbes, the responses of a biphenyl-degrading bacterium to single-walled carbon nanotubes (SWCNTs), multi-walled carbon nanotubes (MWCNTs) and carboxyl single-walled carbon nanotubes (SWCNT-COOHs) were investigated. Electron microscopy, viability test, cellular membrane integrity, and oxidative stress analyses indicated that CNT toxicity was mainly caused by physical piercing. Apart from antibacterial activities, the experimental results showed that CNTs enhanced cell growth and biphenyl degradation at certain concentrations (1.0–1.5 mg/L). The CNTs aggregated and adsorbed cells and biphenyl to form a CNTs-cells-biphenyl coexisting system, thus it created a suitable microenvironment for cell attachment and proliferation where the cells could utilize biphenyl easier for their growth. To the best of our knowledge, this is the first report about CNTs’ impact on biodegradation efficacy and growth of aromatic-degrading bacterium.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Brady-Estévez AS, Kang S, Elimelech M (2008) A single-walled-carbon-nanotube filter for removal of viral and bacterial pathogens. Small 4:481–484
Brar SK, Verma M, Tyagi R, Surampalli R (2010) Engineered nanoparticles in wastewater and wastewater sludge-evidence and impacts. Waste Manag 30:504–520
Chen X, Tam UC, Czlapinski JL, Lee GS, Rabuka D, Zettl A, Bertozzi CR (2006) Interfacing carbon nanotubes with living cells. J Am Chem Soc 128:6292–6293
Chen W, Duan L, Zhu D (2007) Adsorption of polar and nonpolar organic chemicals to carbon nanotubes. Environ Sci Technol 41:8295–8300
Choi O, Hu Z (2008) Size dependent and reactive oxygen species related nanosilver toxicity to nitrifying bacteria. Environ Sci Technol 42:4583–4588
Deng S, Upadhyayula VK, Smith GB, Mitchell MC (2008) Adsorption equilibrium and kinetics of microorganisms on single-wall carbon nanotubes. IEEE Sensors J 8:954–962
Dubey GP, Ben-Yehuda S (2011) Intercellular nanotubes mediate bacterial communication. Cell 144:590–600
Dumortier H, Lacotte S, Pastorin G, Marega R, Wu W, Bonifazi D, Briand J-P, Prato M, Muller S, Bianco A (2006) Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells. Nano Lett 6:1522–1528
Fan J, Cai H, Tan W-S (2007) Role of the plasma membrane ROS-generating NADPH oxidase in CD34+ progenitor cells preservation by hypoxia. J Biotechnol 130:455–462
Goyal D, Zhang X, Rooney-Varga J (2010) Impacts of single-walled carbon nanotubes on microbial community structure in activated sludge. Lett Appl Microbiol 51:428–435
Huang W, Wang Y, Luo G, Wei F (2003) 99.9 % purity multi-walled carbon nanotubes by vacuum high-temperature annealing. Carbon 41:2585–2590
Huang Z, Zheng X, Yan D, Yin G, Liao X, Kang Y, Yao Y, Huang D, Hao B (2008) Toxicological effect of ZnO nanoparticles based on bacteria. Langmuir 24:4140–4144
Iijima S (1991) Helical microtubules of graphitic carbon. Nature 354:56–58
Jones N, Ray B, Ranjit KT, Manna AC (2008) Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiol Lett 279:71–76
Kang S, Pinault M, Pfefferle LD, Elimelech M (2007) Single-walled carbon nanotubes exhibit strong antimicrobial activity. Langmuir 23:8670–8673
Kang S, Herzberg M, Rodrigues DF, Elimelech M (2008a) Antibacterial effects of carbon nanotubes: size does matter! Langmuir 24:6409–6413
Kang S, Mauter MS, Elimelech M (2008b) Physicochemical determinants of multiwalled carbon nanotube bacterial cytotoxicity. Environ Sci Technol 42:7528–7534
Kong C, Wang L, Li P, Qu Y, Tang H, Wang J, Zhou H, Ma Q, Zhou J, Xu P (2013) Genome sequence of Dyella ginsengisoli strain LA-4, an efficient degrader of aromatic compounds. Genome Announc 1:e00961–13
Li Q, Mahendra S, Lyon DY, Brunet L, Liga MV, Li D, Alvarez PJ (2008) Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications. Water Res 42:4591–4602
Li A, Qu Y, Zhou J, Gou M (2009) Isolation and characteristics of a novel biphenyl-degrading bacterial strain, Dyella ginsengisoli LA-4. J Environ Sci 21:211–217
Liu S, Wei L, Hao L, Fang N, Chang MW, Xu R, Yang Y, Chen Y (2009a) Sharper and faster “nano darts” kill more bacteria: a study of antibacterial activity of individually dispersed pristine single-walled carbon nanotube. ACS Nano 3:3891–3902
Liu Z, Tabakman S, Welsher K, Dai H (2009b) Carbon nanotubes in biology and medicine: in vitro and in vivo detection, imaging and drug delivery. Nano Res 2:85–120
Pasquini LM, Sekol RC, Taylor AD, Pfefferle LD, Zimmerman JB (2013) Realizing comparable oxidative and cytotoxic potential of single-and multiwalled carbon nanotubes through annealing. Environ Sci Technol 47:8775–8783
Qu Y, Ma Q, Deng J, Shen W, Zhang X, He Z, Nostrand J, Zhou J (2015) Responses of microbial communities to single-walled carbon nanotubes in phenol wastewater treatment systems. Environ Sci Technol 49:4627–4635
Rodrigues DF, Elimelech M (2010) Toxic effects of single-walled carbon nanotubes in the development of E. coli biofilm. Environ Sci Technol 44:4583–4589
Ruiz ON, Fernando KS, Wang B, Brown NA, Luo PG, McNamara ND, Vangsness M, Sun Y-P, Bunker CE (2011) Graphene oxide: a nonspecific enhancer of cellular growth. ACS Nano 5:8100–8107
Salvetat J-P, Briggs GAD, Bonard J-M, Bacsa RR, Kulik AJ, Stöckli T, Burnham NA, Forró L (1999) Elastic and shear moduli of single-walled carbon nanotube ropes. Phys Rev Lett 82:944
Smith MR, Bittner EW, Shi W, Johnson JK, Bockrath BC (2003) Chemical activation of single-walled carbon nanotubes for hydrogen adsorption. J Phys Chem B 107:3752–3760
Su H-L, Lin S-H, Wei J-C, Pao I-C, Chiao S-H, Huang C-C, Lin S-Z, Lin J-J (2011) Novel nanohybrids of silver particles on clay platelets for inhibiting silver-resistant bacteria. PLoS One 6, e21125
Suresh AK, Pelletier DA, Doktycz MJ (2013) Relating nanomaterial properties and microbial toxicity. Nanoscale 5:463–474
Tong Z, Bischoff M, Nies LF, Myer P, Applegate B, Turco RF (2012) Response of soil microorganisms to as-produced and functionalized single-wall carbon nanotubes (SWNTs). Environ Sci Technol 46:13471–13479
Yang K, Xing B (2010) Adsorption of organic compounds by carbon nanomaterials in aqueous phase: Polanyi theory and its application. Chem Rev 110:5989–6008
Yang K, Zhu L, Xing B (2006) Enhanced soil washing of phenanthrene by mixed solutions of TX100 and SDBS. Environ Sci Technol 40:4274–4280
Yang C, Mamouni J, Tang Y, Yang L (2010) Antimicrobial activity of single-walled carbon nanotubes: length effect. Langmuir 26:16013–16019
Zhang S, Shao T, Bekaroglu SSK, Karanfil T (2010) Adsorption of synthetic organic chemicals by carbon nanotubes: effects of background solution chemistry. Water Res 44:2067–2074
Zhang Y, Xu Y, Li Z, Chen T, Lantz SM, Howard PC, Paule MG, Slikker W Jr, Watanabe F, Mustafa T (2011) Mechanistic toxicity evaluation of uncoated and PEGylated single-walled carbon nanotubes in neuronal PC12 cells. ACS Nano 5:7020–7033
Zhu B, Xia X, Xia N, Zhang S, Guo X (2014) Modification of fatty acids in membranes of bacteria: implication for an adaptive mechanism to the toxicity of carbon nanotubes. Environ Sci Technol 48:4086–4095
This work was supported by the National Natural Science Foundation of China (No. 21176040), the Program for New Century Excellent Talents in University (No. NCET-13-0077), and the Fundamental Research Funds for the Central Universities (No. DUT14YQ107).
Responsible editor: Philippe Garrigues
Electronic supplementary material
Below is the link to the electronic supplementary material.
(DOCX 3033 kb)
About this article
Cite this article
Qu, Y., Wang, J., Zhou, H. et al. Concentration-dependent effects of carbon nanotubes on growth and biphenyl degradation of Dyella ginsengisoli LA-4. Environ Sci Pollut Res 23, 2864–2872 (2016). https://doi.org/10.1007/s11356-015-5532-1
- Carbon nanotubes
- Functional bacterium