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Environmental Science and Pollution Research

, Volume 22, Issue 23, pp 18601–18606 | Cite as

Effects of As2O3 on DNA methylation, genomic instability, and LTR retrotransposon polymorphism in Zea mays

  • Filiz Aygun Erturk
  • Murat Aydin
  • Burcu SigmazEmail author
  • M. Sinan Taspinar
  • Esra Arslan
  • Guleray Agar
  • Semra Yagci
Research Article

Abstract

Arsenic is a well-known toxic substance on the living organisms. However, limited efforts have been made to study its DNA methylation, genomic instability, and long terminal repeat (LTR) retrotransposon polymorphism causing properties in different crops. In the present study, effects of As2O3 (arsenic trioxide) on LTR retrotransposon polymorphism and DNA methylation as well as DNA damage in Zea mays seedlings were investigated. The results showed that all of arsenic doses caused a decreasing genomic template stability (GTS) and an increasing Random Amplified Polymorphic DNAs (RAPDs) profile changes (DNA damage). In addition, increasing DNA methylation and LTR retrotransposon polymorphism characterized a model to explain the epigenetically changes in the gene expression were also found. The results of this experiment have clearly shown that arsenic has epigenetic effect as well as its genotoxic effect. Especially, the increasing of polymorphism of some LTR retrotransposon under arsenic stress may be a part of the defense system against the stress.

Keywords

As2O3 DNA methylation LTR retrotransposons polymorphism Genomic instability Zea mays 

Notes

Acknowledgments

The authors are thankful to the Ataturk University Research Project Foundation (contract no: 2013/146) for financial support of this study.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Filiz Aygun Erturk
    • 1
  • Murat Aydin
    • 2
  • Burcu Sigmaz
    • 1
    Email author
  • M. Sinan Taspinar
    • 3
  • Esra Arslan
    • 1
  • Guleray Agar
    • 1
  • Semra Yagci
    • 1
  1. 1.Department of Biology, Faculty of ScienceAtatürk UniversityErzurumTurkey
  2. 2.Department of Field Crops, Faculty of AgricultureAtaturk UniversityErzurumTurkey
  3. 3.Department of Agricultural Biotechnology, Faculty of AgricultureAtaturk UniversityErzurumTurkey

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