Geosmin induces genomic instability in the mammalian cell microplate-based comet assay
- 132 Downloads
Geosmin (GEO) (trans-1,10-dimethyl-trans-9-decalol) is a metabolite that renders earthy and musty taste and odor to water. Data of GEO genotoxicity on mammalian cells are scarce in the literature. Thus, the present study assessed the genotoxicity of GEO on Chinese hamster ovary (CHO) cells in the microplate-based comet assay. The percent of tail DNA (tail intensity (TI)), tail moment (TM), and tail length (TL) were used as parameters for DNA damage assessment. The results demonstrated that concentrations of GEO of 30 and 60 μg/mL were genotoxic to CHO cells after 4- and 24-h exposure periods, in all parameters evaluated, such as TI, TM, and TL. Additionally, GEO 15 μg/mL was genotoxic in the three parameters only in the 24-h exposure time. The same was observed for GEO 7.5 μg/mL, which induced significant DNA damage observed as TI in the 24-h treatment. The results present evidence that exposure to GEO may be associated with genomic instability in mammalian cells.
KeywordsComet assay Geosmin Tail intensity Tail moment Tail length CHO cells
This work was supported by Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Conflict of interest
The authors declare that they have no conflict of interest.
- Belsito D, Bickers D, Bruze M, Calow P, Greim H, Hanifin JM, Rogers AE et al (2008) A toxicologic and dermatologic assessment of cyclic and non-cyclic terpene alcohols when used as fragrance ingredients. Food Chem Toxicol 46:s1–s71Google Scholar
- De Freitas AM, Sirtori C, Peralta-Zamora PG (2008) Avaliação do potencial de processos oxidativos avançados para remediação de águas contaminadas com geosmina e 2-MIB. Quim Nov. 31:75–78Google Scholar
- Di Bernardo L (1995) Algas e suas influências na qualidade das águas e nas tecnologias de tratamento. ABES, Rio de JaneiroGoogle Scholar
- FUNASA Fundação Nacional de Saúde (2003) Cianobactérias Tóxicas na Água para Consumo Humano na Saúde Pública e Processo de Remoção em Água para Consumo Humano. Ministério da Saúde, BrasíliaGoogle Scholar
- OECD (2014) In vitro mammalian cell micronucleus test, test guideline no. 487, OECD Guidelines for Testing of Chemicals, Paris. Available from: http://www.oecd.org/env/testguidelines
- Rittmann BE, Gantzer CJ, Montiell A (1995) Biological treatment to control taste-and-odor compounds in drinking water treatment. In: Suffet IH, Mallevialle J, Kawczynski E (eds) Advances in taste-and-odor treatment and control. American Water Works Association Research Foundation, Denver, pp 209–246Google Scholar