Environmental Science and Pollution Research

, Volume 23, Issue 2, pp 1224–1233 | Cite as

Modulatory role of mineral nutrients on cadmium accumulation and stress tolerance in Oryza sativa L. seedlings

  • Abin Sebastian
  • M. N. V. PrasadEmail author
Research Article


Cadmium (Cd)-contaminated rice is a serious health concern. In the present study, Cd accumulation and stress responses in Oryza sativa L. cv MTU 7029 seedlings were characterized under varying concentrations of plant nutrients in Hoagland media. It has been found that nutrient supplement modulates Cd accumulation and related stress tolerance while efficacy of each nutrient varies. Supplementation of Fe, Mn, N, Ca, and S were found to reduce Cd accumulation in leaf whereas Mn and Fe supply effect was also observed in roots. Analysis of maximum quantum efficiency of photosynthesis indicated that Fe and S supplements confer highest Cd stress tolerance. The present study highlighted the potential of plant nutrients for minimizing Cd accumulation and its toxicity in rice seedlings.


Cadmium Mineral nutrients Rice Chlorophyll fluorescence Photosynthetic pigments Cadmium toxicity 



Abin Sebastian gratefully acknowledges fellowship received through CSIR-UGC NET and assistance of Jeremy Koelmel, full bright scholar from USA, in our lab for atomic absorption spectroscopy analysis.

Supplementary material

11356_2015_5346_MOESM1_ESM.ppt (5.1 mb)
ESM 1 (PPT 5266 kb)


  1. Arvind P, Prasad MNV (2005) Cadmium–zinc interactions in a hydroponic system using Ceratophyllum demersum L.: adaptive ecophysiology, biochemistry and molecular toxicology. Braz J Plant Physiol 17(1):3–20Google Scholar
  2. Ashley MK, Grant M, Grabov A (2005) Plant responses to potassium deficiencies: a role for potassium transport proteins. J Exp Bot 57(2):425–436CrossRefGoogle Scholar
  3. Chou TS, Chao YY, Huang WD, Hong CY, Kao CH (2010) Effect of magnesium deficiency on antioxidant status and cadmium toxicity in rice seedlings. J Plant Physiol 168:1021–1030CrossRefGoogle Scholar
  4. Clemens S, Antosiewez DM, Ward JM, Schatman DP, Schroeder JI (1998) The plant cDNA LCT1 mediates the uptake of calcium and cadmium in yeast. Proc Natl Acad Sci U S A 95:12043–12044CrossRefGoogle Scholar
  5. Conn SJ, Hocking B, Dayod M, Xu B, Athman A, Henderson S, Aukett L, Conn V, Shearer MK, Fuentes S, Tyerman SD, Gilliham M (2013) Optimizing hydroponic growth systems for nutritional and physiological analysis of Arabidopsis thaliana and other plants. Plant Methods 9:4CrossRefGoogle Scholar
  6. Eriksson JE (1990) A field study on factors influencing Cd levels in soils and in grain of oats and winter wheat. Water Air Soil Pollut 49:355CrossRefGoogle Scholar
  7. Gomes MP, Marques TCLLSM, Soares AM (2013) Cadmium effects on mineral nutrition of the Cd-hyperaccumulator Pfaffia glomerata. Biologia 68(2):223–230CrossRefGoogle Scholar
  8. Grant CA, Bailey LD, Therrien MC (1996) Effect of N, P, and KCl fertilizers on grain yield and Cd concentration of malting barley. Fertil Res 45:153–161CrossRefGoogle Scholar
  9. Grill E, Winnacker EL, Zenk MH (1987) Phytochelatins, a class of heavy-metal binding peptides from plants, are functionally analogous to metallothioneins. Proc Natl Acad Sci U S A 84:439–443CrossRefGoogle Scholar
  10. Gussarsson M, Adalsteinsson S, Jensén P, Asp H (1995) Cadmium and copper interactions on the accumulation and distribution of Cd and Cu in birch (Betula pendula Roth) seedlings. Plant-Soil Interactions Low pH Princ Manag Dev Plant Soil Sci 64:375–377Google Scholar
  11. Hassan MJ, Wang F, Ali S, Zhang G (2005) Toxic effects of cadmium on rice as affected by nitrogen fertilizer form. Plant Soil 277:359–365CrossRefGoogle Scholar
  12. Hoagland D, Arnon DI (1950) The water culture method for growing plants without soils. California Agricultural Experiment Station, Circular, Berkeley, p 347Google Scholar
  13. Jalloh MA, Chen J, Zhen F, Zhang G (2009) Effect of different N fertilizer forms on anti-oxidant capacity and grain yield of rice growing under Cd stress. J Hazard Mater 162:1081–1085CrossRefGoogle Scholar
  14. Jing N, Yanhong C, Jian GM (2009) Effect of boron on cadmium uptake and distribution in two rape varieties. Proceedings of Soil Science Society of China Eleventh National Congress and the Seventh Cross-Strait academic exchanges and Fertilizer Symposium. S 565–4Google Scholar
  15. Kaiser B, Gridley KL, Brady JN, Phillips T, Tyerman S (2005) The role of molybdenum in agricultural plant production. Ann Bot 96:745–754CrossRefGoogle Scholar
  16. Kannan S, Ramani S (1978) Studies of molybdenum absorption and transport in bean and rice. Plant Physiol 62:179–181CrossRefGoogle Scholar
  17. Kashem MDA, Kawai S (2007) Alleviation of cadmium phytotoxicity by magnesium in Japanese mustard spinach. Soil Sci Plant Nutr 53:246–251CrossRefGoogle Scholar
  18. Kumar A, Sebastian A, Prasad MNV, Malec P, Strzalka K (2014) Functional tuning of photosynthetic pigments in response to trace elements. In: Golovko TK, Gruszecki WI, Prasad MNV, Strzalka K (eds) Photosynthetic pigments - chemical, biological and ecological functions. Komi Sci. Ctr, Russia, Syktyvkar, pp 356–381Google Scholar
  19. Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11:591–592CrossRefGoogle Scholar
  20. Mattie MD, McElwee MK, Freedman JH (2008) Mechanism of copper activated transcription: activation of AP-1, and the JNK/SAPK and p38 signal transduction pathways. J Mol Biol 383(5):1008–1018CrossRefGoogle Scholar
  21. McLaughlin MJ, Whatmuff M, Warne M, Heemsbergen D, Barry G, Bell M et al (2006) A field investigation o f solubility and food chain accumulation of biosolid—cadmium across diverse soil types. Environ Chem 3:428–432CrossRefGoogle Scholar
  22. Naza R, Iqbal N, Masood A, Khan MIR, Syeed S, Khan NA (2012) Cadmium toxicity in plants and role of mineral nutrients in its alleviation. Am J Plant Sci 3:1476–1489CrossRefGoogle Scholar
  23. Nevzat E, Okkes A (2013) Nitric oxide alleviates boron toxicity by reducing oxidative damage and growth inhibition in maize seedlings (Zea mays L.). Aust J Crop Sci 7(8):1085–1092Google Scholar
  24. Ololade IA, Ologundudu A (2007) Concentration and bioavailability of cadmium by some plants. Afr J Biotechnol 6(16):1916–1921Google Scholar
  25. Paľove-Balang P, Kisová A, Pavlovkin J, Mistrík I (2006) Effect of manganese on cadmium toxicity in maize seedlings. Plant Soil Environ 52:143–149Google Scholar
  26. Prasad MNV (1995) Cadmium toxicity and tolerance in vascular plants. Environ Exp Bot 35(4):525–545CrossRefGoogle Scholar
  27. Prasad MNV, Nakbanpote W, Sebastian A, Panitlertumpai N, Phadermrod C (2014) Phytomanagement of Padaeng zinc mine waste, Mae Sot district, Tak Province, Thailand. In: Hakeem K, Sabir M, Ozturk M, Mermut A (eds) Soil remediation and plants. Academic Press, p 653–679Google Scholar
  28. Sarwar N, Saifullah, Malhi SS, Zia MH, Naeem A, Bibi S, Farid G (2010) Role of mineral nutrition in minimizing cadmium accumulation by plants. J Sci Food Agric 90:925–937Google Scholar
  29. Sebastian A, Prasad MNV (2014a) Cadmium minimization in rice. A review. Agron Sustain Dev 34:155–173CrossRefGoogle Scholar
  30. Sebastian A, Prasad MNV (2014b) Red and blue lights induced oxidative stress tolerance promote cadmium rhizocomplexation in Oryza sativa L. J Photochem Photobiol B Biol 137:135–143CrossRefGoogle Scholar
  31. Stirbet A, Govindjee (2011) On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and Photosystem II: basics and applications. J Photochem Photobiol B Biol 104:236–257CrossRefGoogle Scholar
  32. Suzuki N (2005) Alleviation by calcium of cadmium induced root growth inhibition in Arabidopsis seedlings. Plant Biotechnol 22:19–25CrossRefGoogle Scholar
  33. Takahara K, Kasajima I, Takahashi H, Hashida S, Itami T, Onoder H, Toki S, Yanagisawa S, Kawai-Yamada M, Uchimiya H (2010) Metabolome and photochemical analysis of rice plants overexpressing Arabidopsis NAD kinase gene. Plant Physiol 152:1863–1873CrossRefGoogle Scholar
  34. Tlustos P, Szakova J, Korinek KP, Avlikova DH, Anc A, Alik J (2006) The effect of liming on cadmium, lead and zinc uptake reduction by spring wheat grown in contaminated soil. Plant Soil Environ 52:16–24Google Scholar
  35. Wang QC, Song H (2009) Calcium protects Trifolium repens L. Seedlings against cadmium stress. Plant Cell Rep 28:1341–1349CrossRefGoogle Scholar
  36. Wang H, Zhao SC, Liu RL, Zhou W, Jin JY (2009) Changes of photosynthetic activities of maize (Zea mays L.) seedlings in response to cadmium stress. Photosynthetica 47:277–283CrossRefGoogle Scholar
  37. Xie HL, Jiang RF, Zhang FS, McGrath SP, Zhao FJ (2009) Effect of nitrogen form on the rhizosphere dynamics and uptake of cadmium and zinc by the hyper accumulator Thlaspi caerulescens. Plant Soil 318:205–215CrossRefGoogle Scholar
  38. Xue Q, Harrison HC (1991) Effect of soil zinc, pH and cultivar uptake in leaf lettuce (Lactuca sativa L. var. crispa). Commun Soil Sci Plant Anal 22:975–991CrossRefGoogle Scholar
  39. Zhou ZS, Song JB, Yang ZM (2012) Genome-wide identification of Brassica napus microRNAs and their targets in response to cadmium. J Exp Bot 63(12):4597–4613CrossRefGoogle Scholar
  40. Zhu ZJ, Sun GW, Fang XZ, Qian QQ, Yang XE (2004) Genotypic differences in effects of cadmium exposure on plant growth and contents of cadmium and elements in 14 cultivars of bai cai. J Environ Sci Health B 39(4):675–687CrossRefGoogle Scholar
  41. Zornoza P, Sánchez-Pardo B, Carpena RRO (2010) Interaction and accumulation of manganese and cadmium in the manganese accumulator Lupinus albus. J Plant Physiol 167:1027–1032CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Department of Plant SciencesUniversity of HyderabadHyderabadIndia

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