Environmental Science and Pollution Research

, Volume 23, Issue 4, pp 3036–3041 | Cite as

Oxidoreductases provide a more generic response to metallic stressors (Cu and Cd) than hydrolases in soil fungi: new ecotoxicological insights

  • Jérémie D. LebrunEmail author
  • Nathalie Demont-Caulet
  • Nathalie Cheviron
  • Karine Laval
  • Isabelle Trinsoutrot-Gattin
  • Christian Mougin
ECOTOX, the INRA's network of ecotoxicologists


The present study investigates the effect of metals on the secretion of enzymes from12 fungal strains maintained in liquid cultures. Hydrolases (acid phosphatase, β-glucosidase, β-galactosidase, and N-acetyl-β-glucosaminidase) and ligninolytic oxidoreductases (laccase, Mn, and lignin peroxidases) activities, as well as biomass production, were measured in culture fluids from fungi exposed to Cu or Cd. Our results showed that all fungi secreted most of the selected hydrolases and that about 50 % of them produced a partial oxidative system in the absence of metals. Then, exposure of fungi to metals led to the decrease in biomass production. At the enzymatic level, Cu and Cd modified the secretion profiles of soil fungi. The response of hydrolases to metals was contrasted and complex and depended on metal, enzyme, and fungal strain considered. By contrast, the metals always stimulated the activity of ligninolytic oxidoreductases in fungal strains. In some of them, oxidoreductases were specifically produced following metal exposure. Fungal oxidoreductases provide a more generic response than hydrolases, constituting thus a physiological basis for their use as biomarkers of metal exposure in soils.


Hydrolases Ligninolytic oxidoreductases Metals Biomarkers Secretion profiles Ecotoxicology 



The present study was supported by INRA and the Conseil Régional de Haute Normandie. The authors thank Ph.D. C. Steinberg and C. Héraud (UMR 1347 Agroécologie, INRA, Dijon, France) for their valuable information about the MIAE collection and Ph.D. C. Novotny (Institute of Microbiology, Prague, Czech Republic) for providing the strain of I. lacteus.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Jérémie D. Lebrun
    • 1
    • 2
    • 3
    Email author
  • Nathalie Demont-Caulet
    • 3
    • 4
  • Nathalie Cheviron
    • 3
  • Karine Laval
    • 2
  • Isabelle Trinsoutrot-Gattin
    • 2
  • Christian Mougin
    • 3
    • 5
  1. 1.Irstea, UR HBAN—EcotoxicologyAntony CedexFrance
  2. 2.Esitpa-Ecole d’Ingénieurs en AgricultureMont-Saint-AignanFrance
  3. 3.INRA, UMR 1402 ECOSYSVersailles CedexFrance
  4. 4.Sorbonne Paris CitéUniversité Paris DiderotParisFrance
  5. 5.AgroParisTech, UMR1402 ECOSYSVersailles CedexFrance

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