Environmental Science and Pollution Research

, Volume 22, Issue 22, pp 18137–18147 | Cite as

Antifungal efficiency of a lipopeptide biosurfactant derived from Bacillus subtilis SPB1 versus the phytopathogenic fungus, Fusarium solani

  • Ines MnifEmail author
  • Ines Hammami
  • Mohamed Ali Triki
  • Manel Cheffi Azabou
  • Semia Ellouze-Chaabouni
  • Dhouha Ghribi
Research Article


Bacillus subtilis SPB1 lipopeptides were evaluated as a natural antifungal agent against Fusarium solani infestation. In vitro antifungal assay showed a minimal inhibitory concentration of about 3 mg/ml with a fungicidal mode of action. In fact, treatment of F. solani by SPB1 lipopeptides generated excessive lyses of the mycelium and caused polynucleation and destruction of the related spores together with a total inhibition of spore production. Furthermore, an inhibition of germination potency accompanied with a high spore blowing was observed. Moreover, in order to be applied in agricultural field, in vivo antifungal activity was proved against the dry rot potato tubers caused by F. solani. Preventive treatment appeared as the most promising as after 20 days of fungi inoculation, rot invasion was reduced by almost 78 %, in comparison to that of non-treated one. When treating infected tomato plants, disease symptoms were reduced by almost 100 % when applying the curative method. Results of this study are very promising as it enables the use of the crude lipopeptide preparation of B. subtilis SPB1 as a potent natural fungicide that could effectively control the infection of F. solani in tomato and potato tubers at a concentration similar to the commercial fungicide hymexazol and therefore prevent the damage of olive tree.


F. solani SPB1 lipopeptide Antifungal activity Bio-control Potato tuber rot Tomato root rot 



This work has been supported by grants from the Tunisian Ministry of Higher Education, Scientific Research and Technology and the Tunisian Ministry of Agriculture.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Ines Mnif
    • 1
    • 2
    Email author
  • Ines Hammami
    • 3
  • Mohamed Ali Triki
    • 4
  • Manel Cheffi Azabou
    • 4
  • Semia Ellouze-Chaabouni
    • 1
  • Dhouha Ghribi
    • 1
    • 2
  1. 1.Unit “Enzymes and Bioconversion”, National School of Engineers of SfaxUniversity of SfaxSfaxTunisia
  2. 2.Higher Institute of Biotechnology of SfaxUniversity of SfaxSfaxTunisia
  3. 3.Higher School of Agriculture of KefKefTunisia
  4. 4.Laboratory “Amélioration et Protection des Ressources Génétiques de l’Olivier”, Sfax BP1087Institut of Olivier, IRESA - University of SfaxSfaxTunisia

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