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Environmental Science and Pollution Research

, Volume 22, Issue 19, pp 14882–14890 | Cite as

Simultaneous detection of imidacloprid and parathion by the dual-labeled time-resolved fluoroimmunoassay

  • Haiyan Shi
  • Enze Sheng
  • Lu Feng
  • Liangliang Zhou
  • Xiude Hua
  • Minghua Wang
Research Article

Abstract

A highly sensitive direct dual-labeled time-resolved fluoroimmunoassay (TRFIA) to detect parathion and imidacloprid simultaneously in food and environmental matrices was developed. Europium (Eu3+) and samarium (Sm3+) were used as fluorescent labels by coupling separately with L1-Ab and A1P1-Ab. Under optimal assay conditions, the half-maximal inhibition concentration (IC50) and limit of detection (LOD, IC10) were 10.87 and 0.025 μg/L for parathion and 7.08 and 0.028 μg/L for imidacloprid, respectively. The cross-reactivities (CR) were negligible except for methyl-parathion (42.4 %) and imidaclothiz (103.4 %). The average recoveries of imidacloprid ranged from 78.9 to 104.2 % in water, soil, rice, tomato, and Chinese cabbage with a relative standard deviation (RSD) of 2.4 to 11.6 %, and those of parathion were from 81.5 to 110.9 % with the RSD of 3.2 to 10.5 %. The results of TRFIA for the authentic samples were validated by comparison with gas chromatography (GC) analyses, and satisfactory correlations (parathion: R 2 = 0.9918; imidacloprid: R 2 = 0.9908) were obtained. The results indicate that the dual-labeled TRFIA is convenient and reliable to detect parathion and imidacloprid simultaneously in food and environmental matrices.

Keywords

Imidacloprid Parathion Europium Samarium Time-resolved fluoroimmunoassay 

Notes

Acknowledgments

This project was supported by the National Natural Science Foundation of China (31301692) and the Fundamental Research Funds for the Central Universities (KJQN201433 and KYZ201305).

Compliance with ethical standards

The manuscript has not been submitted to more than one journal for simultaneous consideration. The manuscript has not been published previously (partly or in full). A single study is not split up into several parts to increase the quantity of submissions and submitted to various journals or to one journal over time. No data have been fabricated or manipulated (including images) to support our conclusions. No data, text, or theories by others are presented as if they were the authors’ own (“plagiarism”).

All authors whose names appear on the submission have contributed sufficiently to the scientific work and therefore share collective responsibility and accountability for the results. They have reviewed this manuscript and approved to submit to your journal.

Supplementary material

11356_2015_4697_MOESM1_ESM.docx (212 kb)
Fig. S1 (DOCX 211 kb)
11356_2015_4697_MOESM2_ESM.docx (397 kb)
Fig. S2 (DOCX 397 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Haiyan Shi
    • 1
  • Enze Sheng
    • 1
  • Lu Feng
    • 1
  • Liangliang Zhou
    • 1
  • Xiude Hua
    • 1
  • Minghua Wang
    • 1
  1. 1.Department of Pesticide Science, College of Plant Protection, Jiangsu Key Laboratory of Pesticide Science, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of EducationNanjing Agricultural UniversityNanjingPeople’s Republic of China

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