Environmental Science and Pollution Research

, Volume 22, Issue 17, pp 13477–13485 | Cite as

Microbial community structure in a dual chamber microbial fuel cell fed with brewery waste for azo dye degradation and electricity generation

  • Waheed Miran
  • Mohsin Nawaz
  • Avinash Kadam
  • Seolhye Shin
  • Jun Heo
  • Jiseon Jang
  • Dae Sung Lee
Research Article
First Online:
Received:
Accepted:

Abstract

The expansion in knowledge of the microbial community structure can play a vital role in the electrochemical features and operation of microbial fuel cells (MFCs). In this study, bacterial community composition in a dual chamber MFC fed with brewery waste was investigated for simultaneous electricity generation and azo dye degradation. A stable voltage was generated with a maximum power density of 305 and 269 mW m−2 for brewery waste alone (2000 mg L−1) and after the azo dye (200 mg L−1) addition, respectively. Azo dye degradation was confirmed by Fourier transform infrared spectroscopy (FT-IR) as peak corresponding to –N=N– (azo) bond disappeared in the dye metabolites. Microbial communities attached to the anode were analyzed by high-throughput 454 pyrosequencing of the 16S rRNA gene. Microbial community composition analysis revealed that Proteobacteria (67.3 %), Betaproteobacteria (30.8 %), and Desulfovibrio (18.3 %) were the most dominant communities at phylum, class, and genus level, respectively. Among the classified genera, Desulfovibrio most likely plays a major role in electron transfer to the anode since its outer membrane contains c-type cytochromes. At the genus level, 62.3 % of all sequences belonged to the unclassified category indicating a high level of diversity of microbial groups in MFCs fed with brewery waste and azo dye.

Highlights

• Azo dye degradation and stable bioelectricity generation was achieved in the MFC.

• Anodic biofilm was analyzed by high-throughput pyrosequencing of the 16S rRNA gene.

• Desulfovibrio (18.3 %) was the dominant genus in the classified genera.

• Of the genus, 62.3 % were unclassified, thereby indicating highly diverse microbes.

Graphical Abstract

A schematic diagram of a dual chamber microbial fuel cell for azo dye degradation and current generation (with microbial communities at anode electrode)

Keywords

Microbial fuel cell Brewery wastewater Dye degradation Bioelectricity Microbial community analysis Pyrosequencing 
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Notes

Acknowledgments

This work was supported by the Human Resource Training Program for Regional Innovation and Creativity through the Ministry of Education and National Research Foundation (NRF) of Korea (NRF-2014H1C1A1066929). This study was also supported by grants (NRF-2013R1A1A4A01008000 and NRF-2009-0093819) through the ME and NRF of Korea. This research was also supported by the NRF grant by the Korea government (MSIP) (NRF-2015M2A7A1000194).

Supplementary material

11356_2015_4582_MOESM1_ESM.docx (198 kb)
ESM 1 (DOCX 197 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Waheed Miran
    • 1
  • Mohsin Nawaz
    • 1
  • Avinash Kadam
    • 1
  • Seolhye Shin
    • 1
  • Jun Heo
    • 1
  • Jiseon Jang
    • 1
  • Dae Sung Lee
    • 1
  1. 1.Department of Environmental EngineeringKyungpook National UniversityDaeguRepublic of Korea

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