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Environmental Science and Pollution Research

, Volume 22, Issue 20, pp 15260–15272 | Cite as

Dynamics of bacterial assemblages and removal of polycyclic aromatic hydrocarbons in oil-contaminated coastal marine sediments subjected to contrasted oxygen regimes

  • Cécile MilitonEmail author
  • Ronan Jézéquel
  • Franck Gilbert
  • Yannick Corsellis
  • Léa Sylvi
  • Cristiana Cravo-Laureau
  • Robert Duran
  • Philippe Cuny
DECAPAGE Project: Hydrocarbon degradation in coastal sediments*

Abstract

To study the impact of oxygen regimes on the removal of polycylic aromatic hydrocarbons (PAHs) in oil-spill-affected coastal marine sediments, we used a thin-layer incubation method to ensure that the incubated sediment was fully oxic, anoxic, or was influenced by oxic-anoxic switches without sediment stirring. Hydrocarbon content and microbial assemblages were followed during 60 days to determine PAH degradation kinetics and microbial community dynamics according to the oxygenation regimes. The highest PAH removal, with 69 % reduction, was obtained at the end of the experiment under oxic conditions, whereas weaker removals were obtained under oscillating and anoxic conditions (18 and 12 %, respectively). Bacterial community structure during the experiment was determined using a dual 16S rRNA genes/16S rRNA transcripts approach, allowing the characterization of metabolically active bacteria responsible for the functioning of the bacterial community in the contaminated sediment. The shift of the metabolically active bacterial communities showed that the selection of first responders belonged to Pseudomonas spp. and Labrenzia sp. and included an unidentified Deltaproteobacteria—irrespective of the oxygen regime—followed by the selection of late responders adapted to the oxygen regime. A novel unaffiliated phylotype (B38) was highly active during the last stage of the experiment, at which time, the low-molecular-weight (LMW) PAH biodegradation rates were significant for permanent oxic- and oxygen-oscillating conditions, suggesting that this novel phylotype plays an active role during the restoration phase of the studied ecosystem.

Keywords

Oxic/anoxic oscillation Aerobic and anaerobic hydrocarbon degradation PAHs Marine sediment Bacterial communities DGGE Dual 16S rRNA/rRNA gene 

Notes

Acknowledgments

This research was funded by the French National Agency (ANR) under the DHYVA (ANR 2006 SEST 09) and DECAPAGE (ANR 2011 CESA 006 01) projects. We thank Patricia Bonin for many fruitful and interesting exchanges.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Cécile Militon
    • 1
    • 6
    Email author
  • Ronan Jézéquel
    • 2
  • Franck Gilbert
    • 3
    • 4
  • Yannick Corsellis
    • 1
  • Léa Sylvi
    • 1
  • Cristiana Cravo-Laureau
    • 5
  • Robert Duran
    • 5
  • Philippe Cuny
    • 1
  1. 1.Aix Marseille Université, CNRS/INSU, Université de Toulon, IRD, Mediterranean Institute of Oceanography (MIO) UM 110MarseilleFrance
  2. 2.Centre de Documentation, de Recherche et d’Expérimentations sur les pollutions accidentelles des EauxBrestFrance
  3. 3.Université de Toulouse; INP, UPS; EcoLab (Laboratoire écologie fonctionnelle et environnement)ToulouseFrance
  4. 4.CNRS; EcoLabToulouseFrance
  5. 5.Equipe Environnement et Microbiologie, MELODY groupUniversité de Pau et des Pays de l’Adour, IPREM UMR CNRS 5254Pau CedexFrance
  6. 6.Campus de LuminyMarseille Cedex 09France

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