Biodegradation of pesticides using fungi species found in the aquatic environment
- 657 Downloads
Relatively limited attention has been given to the presence of fungi in the aquatic environment compared to their occurrence in other matrices. Taking advantage and recognizing the biodegradable capabilities of fungi is important, since these organisms may produce many potent enzymes capable of degrading toxic pollutants. Therefore, the aim of this study was to evaluate the potential ability of some species of filamentous fungi that occur in the aquatic environment to degrade pesticides in untreated surface water. Several laboratory-scale experiments were performed using the natural microbial population present in the aquatic environment as well as spiked fungi isolates that were found to occur in different water matrices, to test the ability of fungi to degrade several pesticides of current concern (atrazine, diuron, isoproturon and chlorfenvinphos). The results obtained in this study showed that, when spiked in sterile natural water, fungi were able to degrade chlorfenvinphos to levels below detection and unable to degrade atrazine, diuron and isoproturon. Penicillium citrinum, Aspergillus fumigatus, Aspergillus terreus and Trichoderma harzianum were found to be able to resist and degrade chlorfenvinphos. These fungi are therefore expected to play an important role in the degradation of this and other pollutants present in the aquatic environment.
KeywordsFungi Biodegradation Pesticides Surface water
Financial support from Fundação para a Ciência e a Tecnologia—through the project PTDC/AAC-AMB/108303/2008, the grant PEst-OE/EQB/LA0004/2011 and the fellowship BPD/26990/2006—is gratefully acknowledged.
We also thank EPAL as participant institution of the project PTDC/AAC-AMB/108303/2008 for supplying the untreated real water matrices used and for the analysis of the breakdown products.
Vanessa J. Pereira thanks the Department of Geography and Environmental Engineering at Johns Hopkins University for hosting her as a Visiting Scholar during the academic year 2012/2013.
- Bouchiat R, Veignie E, Grizard D, Soebert C, Vigier M, Rafin C (2015) Ability of filamentous fungi to degrade four emergent water priority pollutants. Desalin Water Treat. doi: 10.1080/19443994.2015.1013508
- Evans CS, Hedger JN (2001) Degradation of plant cell wall polymers. In: Gadd GM (ed) Fungi in bioremediation. Cambridge University Press, Cambridge, p 26Google Scholar
- Glass NL, Donaldson GC (1994) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61:1323–1330Google Scholar
- Madigan MT, Martinko JM, Dunlap PV, Clark DP (2012) Brock biology of microorganisms. Pearson Prentice Hall, Upper Saddle, p 992Google Scholar
- Mitchell R, Gu J-D (2010) Environmental microbiology. Wiley Blackwell, Hoboken, pp 410Google Scholar
- O’Donnell K, Sarver BAJ, Brandt M, Chang DC, Noble-Wang J, Park BJ, Sutton DA, Benjamin L, Lindsley M, Padhye A, Geiser DM, Ward TJ (2007) Phylogenetic diversity and microsphere array-based genotyping of human pathogenic Fusaria, including isolates from the multistate contact lens-associated U.S. keratitis outbreaks of 2005 and 2006. J Clin Microbiol 45:2235–2248CrossRefGoogle Scholar
- Samson RA, Seifert KA, Kuijpers AFA, Houbraken JAMP, Frisvad JC (2004) Phylogenetic analysis of Penicillium subgenus Penicillium using partial β-tubulin sequences. Stud Mycol 49:175–200Google Scholar
- Saravanan R, Sivakumar T (2013) Biodiversity and biodegradation potentials of fungi isolated from marine systems of East Coast of Tamil Nadu, India. Int J Curr Microbiol Appl Sci 2:192–201Google Scholar
- Silambarasan S, Abraham J (2012) Ecofriendly method for bioremediation of chlorpyrifos from agricultural soil by novel fungus Aspergillus terreus JAS1. Water Air Soil Pollut 224:1–11Google Scholar
- Uhnáková B, Ludwig R, Pěknicová J, Homolka L, Lisá L, Šulc M, Petříčková A, Elzeinová F, Pelantová H, Monti D, Křen V, Haltrich D, Martínková L (2011) Biodegradation of tetrabromobisphenol A by oxidases in basidiomycetous fungi and estrogenic activity of the biotransformation products. Bioresour Technol 102:9409–9415CrossRefGoogle Scholar