Environmental Science and Pollution Research

, Volume 22, Issue 15, pp 11349–11359 | Cite as

24-Epibrassinolide mitigates the adverse effects of manganese induced toxicity through improved antioxidant system and photosynthetic attributes in Brassica juncea

  • Qazi Fariduddin
  • Mumtaz Ahmed
  • Bilal A. Mir
  • Mohammad Yusuf
  • Tanveer A. Khan
Research Article


The objective of this study was to establish relationship between manganese-induced toxicity and antioxidant system response in Brassica juncea plants and also to investigate whether brassinosteroids activate antioxidant system to confer tolerance to the plants affected with manganese induced oxidative stress. Brassica juncea plants were administered with 3, 6, or 9 mM manganese at 10-day stage for 3 days. At 31-day stage, the seedlings were sprayed with deionized water (control) or 10−8 M of 24-epibrassinolide, and plants were harvested at 45-day stage to assess growth, leaf gas-exchange traits, and biochemical parameters. The manganese treatments diminished growth along with photosynthetic attributes and carbonic anhydrase activity in the concentration-dependent manner, whereas it enhanced lipid peroxidation, electrolyte leakage, accumulation of H2O2 as well as proline, and various antioxidant enzymes in the leaves of Brassica juncea which were more pronounced at higher concentrations of manganese. However, the follow-up application of 24-epibrassinolide to the manganese stressed plants improved growth, water relations, and photosynthesis and further enhanced the various antioxidant enzymes viz. catalase, peroxidase, and superoxide dismutase and content of proline. The elevated level of antioxidant enzymes as well as proline could have conferred tolerance to the manganese-stressed plants resulting in improved growth and photosynthetic attributes.


Manganese toxicity Brassinosteroids Photosynthesis Lipid peroxidation Antioxidant enzymes 



Financial assistance rendered by Council of Science and Technology, U.P. Lucknow, India in the form of Research Project [Project No. CST/D-615] is gratefully acknowledged by Qazi Fariduddin.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Qazi Fariduddin
    • 1
  • Mumtaz Ahmed
    • 1
  • Bilal A. Mir
    • 1
  • Mohammad Yusuf
    • 1
  • Tanveer A. Khan
    • 1
  1. 1.Plant Physiology and Biochemistry Section, Department of BotanyAligarh Muslim UniversityAligarhIndia

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