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Dynamic of bacterial communities attached to lightened phytodetritus

  • Microbial Ecology of the Continental and Coastal Environments
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Abstract

The effects of singlet oxygen (1O2) transfer to bacteria attached on phytodetritus were investigated under laboratory-controlled conditions. For this purpose, a nonaxenic culture of Emiliania huxleyi in late stationary phase was studied for bacterial viability. Our results indicated that only 9 ± 3 % of attached bacteria were alive compared to 46 ± 23 % for free bacteria in the E. huxleyi culture. Apparently, under conditions of low irradiance (36 W m−2), during the culture, the cumulative dose received (22,000 kJ m−2) was sufficiently important to induce an efficient 1O2 transfer to attached bacteria during the senescence of E. huxleyi cells. At this stage, attached bacteria appeared to be dominated by pigmented bacteria (Maribacter, Roseobacter, Roseovarius), which should resist to 1O2 stress probably due to their high contents of carotenoids. After subsequent irradiation of the culture until fully photodegradation of chlorophyll, DGGE analyses showed that the diversity of bacteria attached to E. huxleyi cells is modified by light. Photooxidative alterations of bacteria were confirmed by the increasing amounts of cis-vaccenic photoproducts (bacterial marker) per bacteria observed during irradiation time. Interestingly, preliminary chemotaxis experiments showed that Shewanella oneidensis considered here as a model of motile bacteria was attracted by phytodetritus producing or not 1O2. This lack of repulsive effects could explain the high mortality rate of bacteria measured on E. huxleyi cells.

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Acknowledgments

This work was performed in the framework of the transverse axis DEBAT of the MIO and funded by research grants from the PHOTOMED (MERMEX/MISTRALS WP4) and TRANSPHYTOBAC (EC2CO-Microbien) research projects. The PhD scholarship for MP was provided by Aix Marseille University (EDSV 251) and the Ministry of Research and Technology. Thanks are due to two anonymous reviewers for their useful and constructive comments.

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  1. Aix-Marseille Université, Mediterranean Institute of Oceanography (MIO), Université du Sud Toulon-Var, 83957, CNRS-INSU/IRD UM 110, 13288, Marseille, Cedex 9, France

    Morgan Petit, Patricia Bonin, Rémi Amiraux, Valérie Michotey, Sophie Guasco, Frédéric Vaultier & Jean-François Rontani

  2. Aix-Marseille Université, CNRS, Laboratoire de Bioénergétique et Ingénierie des Protéines, UMR7281, Institut de Microbiologie de la Méditerranée, 13402, Marseille, France

    Joshua Armitano, Cécile Jourlin-Castelli & Vincent Méjean

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  1. Morgan Petit
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  2. Patricia Bonin
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  3. Rémi Amiraux
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  4. Valérie Michotey
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Correspondence to Patricia Bonin.

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Petit, M., Bonin, P., Amiraux, R. et al. Dynamic of bacterial communities attached to lightened phytodetritus. Environ Sci Pollut Res 22, 13681–13692 (2015). https://doi.org/10.1007/s11356-015-4209-0

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