Environmental Science and Pollution Research

, Volume 22, Issue 11, pp 8471–8479 | Cite as

Effects of Fructus ligustri lucidi on the growth, cell integrity, and metabolic activity of the Microcystis aeruginosa

Research Article

Abstract

Agricultural waste has been used in the treatment of cyanobacterial bloom because of its environmental friendly and cost-efficient characteristics. In this work, the effects of Fructus ligustri lucidi (FLL) on the growth inhibition, physiological properties, algicidal property, and cell ultrastructure of Microcystis aeruginosa were investigated for the first time. The alga was efficiently inhibited by FLL at the dosages from 0.25 to 4.0 g L−1, and the Chl-a fluorescence and metabolic activity of cells also declined gradually. During 25 days incubation time, the inhibition ratio of 0.25 g L−1 dosage increased from 8 to 68 %, the percentage of intact cells decreased from 94.4 to 59.8 %, the inhibition ratio of 2.0 and 4.0 g L−1 dosages was nearly 100 %, and the cell membranes were completely broken. The results of Chl-a, propidium iodide (PI) staining, fluorescein diacetate (FDA) staining, and transmission electron microscopy (TEM) assays were consistent with that of growth inhibition tests. The new medium test with the PI staining test suggested that FLL may act as an algicidal agent which can inhibit the growth of M. aeruginosa in the acute time. Consequently, FLL could be an excellent choice in the treatment of eutrophic water.

Keywords

Fructus ligustri lucidi Microcystis aeruginosa Growth inhibition Chl-a fluorescence Cell integrity Metabolic activity 

Abbreviations

FLL

Fructus ligustri lucidi

PI

Propidium iodide

FDA

Fluorescein diacetate

TEM

Transmission electron microscopy

FCM

Flow cytometry

IR

Inhibition ratio

PPU

The percentage of PI-unstained cells

MFI

The mean fluorescence intensity

PSC

The proportion of single cell

PCC

The proportion of two connected cells during cell division

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.School of Resources and Environmental EngineeringAnhui UniversityHefeiChina
  2. 2.Institute of Healthy ScienceAnhui UniversityHefeiChina

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