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Environmental Science and Pollution Research

, Volume 22, Issue 9, pp 6932–6940 | Cite as

Occurrence of sulfonamide-, tetracycline-, plasmid-mediated quinolone- and macrolide-resistance genes in livestock feedlots in Northern China

  • Quanhua Mu
  • Jin Li
  • Yingxue Sun
  • Daqing Mao
  • Qing Wang
  • Yi Luo
Research Article

Abstract

Antibiotic resistance genes (ARGs) in livestock feedlots deserve attention because they are prone to transfer to human pathogens and thus pose threats to human health. In this study, the occurrence of 21 ARGs, including tetracycline (tet)-, sulfonamide (sul)-, plasmid-mediated quinolone (PMQR)- and macrolide-resistance (erm) genes were investigated in feces and adjacent soils from chicken, swine, and cattle feedlots in Northern China. PMQR and sul ARGs were the most prevalent and account for over 90.0 % of the total ARGs in fecal samples. Specifically, PMQR genes were the most prevalent, accounting for 59.6 % of the total ARGs, followed by sul ARGs (34.2 %). The percentage of tet ARGs was 3.4 %, and erm ARGs accounted for only 1.9 %. Prevalence of PMQR and sul ARGs was also found in swine and cattle feces. The overall trend of ARG concentrations in feces of different feeding animals was chicken > swine > beef cattle in the studied area. In soils, sul ARGs had the highest concentration and account for 71.1 to 80.2 % of the total ARGs, which is possibly due to the widely distributed molecular carriers (i.e., class one integrons), facilitating sul ARG propagation. Overall, this study provides integrated profiles of various types of ARGs in livestock feedlots and thus provides a reference for the management of antibiotic use in livestock farming.

Keywords

Antibiotic resistance genes Livestock farming Antibiotics 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Nos. 31270542, 21037002, and 21277075), the State Environmental Protection Commonweal Project (201309031), and the Ministry of Education Program for New Century Excellent Talents (NCET-11-0254). The authors owe sincere appreciation to Dr. Fengxia Yang, Dr. Shuai Yu, and Dr. Kangxin He for their kind instructions during the experiments. Special thanks to Dr. Su Chen for providing some of the manure and soil samples.

Conflict of interest

The authors declare no competing financial interests.

Supplementary material

11356_2014_3905_MOESM1_ESM.doc (230 kb)
ESM 1 (DOC 230 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), College of Environmental Science and EngineeringNankai UniversityTianjinChina
  2. 2.Collage of Environmental Science and EngineeringTianjin UniversityTianjinChina

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