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The effect of selenium on the subcellular distribution of antimony to regulate the toxicity of antimony in paddy rice

Abstract

Selenium (Se) can alleviate the toxicity of antimony (Sb) in plants; however, the associated mechanisms have not been fully clarified. In this study, we hypothesize that Se can affect the subcellular distribution of Sb to regulate Sb toxicity. To test our hypothesis, two nested hydroponic experiments were performed by using paddy rice (Fengmeizhan). The results showed that Sb exerted toxic effects on the growth of paddy rice, and Se caused beneficial effects that were limited to the shoot growth. In general, Se and Sb mutually showed antagonistic effects on their uptake and concentrations in different subcellular fractions. However, in some cases, the stimulation effects of Sb on the Se concentration in chlorophyll (Chl) and cytosol (Cy) fractions or of Se on the Sb concentration in the cell wall fraction (Cw) were also observed in the shoots, which might suggest that Sb detoxification by Se is also related to the migration of both Se and Sb in cells. Selenium and Sb were primarily concentrated in the Cw and Cy, suggesting the important roles of these two fractions in detoxifying Se and Sb. When paddy rice was subjected to increasing Sb concentrations and a fixed Se concentration, most of the Se in the shoots was sequestered in the Cy (59.81–79.51 % of total Se) and more Se was transferred into the inner cell from Cw; however, in the roots, Se was primarily concentrated in the Cw (53.28–72.10 %). When paddy rice was exposed to increasing Se concentrations with a fixed Sb concentration, the Cw in both the shoots and roots might play an important role in binding Se, especially in the roots where up to 78.92 % of the total Se was sequestered in the Cw.

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Acknowledgments

This research was supported by the Central Public Research Institute Basic Fund for Research and Development (2014-szjj-frw-08), the National Science Foundation of China (41103075, 41101306), and the Special Fund for Agro-scientific Research in the Public Interest (201203045).

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Correspondence to Renwei Feng.

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Responsible editor: Elena Maestri

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Ding, Y., Wang, R., Guo, J. et al. The effect of selenium on the subcellular distribution of antimony to regulate the toxicity of antimony in paddy rice. Environ Sci Pollut Res 22, 5111–5123 (2015). https://doi.org/10.1007/s11356-014-3865-9

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Keywords

  • Selenium
  • Antimony
  • Subcellular distribution
  • Chlorophyll
  • Uptake inhibition