Environmental Science and Pollution Research

, Volume 22, Issue 22, pp 17267–17279 | Cite as

Changes in protein expression of pacific oyster Crassostrea gigas exposed in situ to urban sewage

  • Fabrício Flores-Nunes
  • Tânia Gomes
  • Rui Company
  • Roberta R. M. Moraes
  • Silvio T. Sasaki
  • Satie Taniguchi
  • Márcia C. Bicego
  • Cláudio M. R. Melo
  • Afonso C. D. Bainy
  • Maria J. Bebianno
Molecular and cellular effects of contamination in aquatic ecosystems
First Online:
Received:
Accepted:

Abstract

The composition and concentration of substances in urban effluents are complex and difficult to measure. These contaminants elicit biological responses in the exposed organisms. Proteomic analysis is a powerful tool in environmental toxicology by evidencing alterations in protein expression due to exposure to contaminants and by providing a useful framework for the development of new potential biomarkers. The aim of this study was to determine changes in protein expression signatures (PES) in the digestive gland of oysters Crassostrea gigas transplanted to two farming areas (LIS and RIB) and to one area contaminated by sanitary sewage (BUC) after 14 days of exposure. This species is one of the most cultivated molluscs in the world. The identified proteins are related to the cytoskeleton (CKAP5 and ACT2), ubiquitination pathway conjugation (UBE3C), G protein-coupled receptor and signal transduction (SVEP1), and cell cycle/division (CCNB3). CKAP5 showed higher expression in oysters kept at BUC in comparison with those kept at the farming areas, while ACT2, UBE3C, SVEP1, and CCNB3 were suppressed. The results suggest that these changes might lead to DNA damage, apoptosis, and interference with the immune system in oyster C. gigas exposed to sewage and give initial information on PES of C. gigas exposed to sanitary sewage, which can subsequently be useful in the development of more sensitive tools for biomonitoring coastal areas, particularly those devoted mainly to oyster farming activities.

Keywords

Urban sewage Crassostrea gigas Proteomic analysis Two-dimensional gel electrophoresis 
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Notes

Acknowledgments

FFN was a fellow doctoral PDEE (CAPES, Process 1452/10-0). The research leading to these results has received funding from the EU project GENERA within the framework of the Marie Curie IRSES Actions (FP7-PEOPLE-2009-IRSES, Proposal no. 247559) and CNPq grant number 483028/2012-6. Analysis of organic contaminants was conducted in partnership with the Laboratory of Organic Chemistry of the Oceanographic Institute of the University of São Paulo (LQO-IO-USP). ACDB, MCB, and CMRM are recipients of CNPq productivity fellowship. MJB is recipient of PVE-CNPq fellowship.

Supplementary material

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Fabrício Flores-Nunes
    • 1
  • Tânia Gomes
    • 2
  • Rui Company
    • 2
  • Roberta R. M. Moraes
    • 1
  • Silvio T. Sasaki
    • 3
  • Satie Taniguchi
    • 3
  • Márcia C. Bicego
    • 3
  • Cláudio M. R. Melo
    • 4
  • Afonso C. D. Bainy
    • 1
  • Maria J. Bebianno
    • 2
  1. 1.Laboratory for Biomarkers of Aquatic Contamination and ImmunochemistryFederal University Santa CatarinaFlorianópolisBrazil
  2. 2.CIMA, Faculty of Science and TechnologyUniversity of AlgarveFaroPortugal
  3. 3.Laboratory of Marine Organic Chemistry, Oceanographic InstituteUniversity of São PauloSão PauloBrazil
  4. 4.Laboratory of Marine MolluscsFederal University of Santa CatarinaFlorianópolisBrazil

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